Hypothetical types of biochemistry

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False-color Cassini radar mosaic of Titan's north polar region; the blue areas are lakes of liquid hydrocarbons.
"The existence of lakes of liquid hydrocarbons on Titan opens up the possibility for solvents and energy sources that are alternatives to those in our biosphere and that might support novel life forms altogether different from those on Earth."—NASA Astrobiology Roadmap 2008

Hypothetical types of biochemistry are forms of biochemistry speculated to be scientifically viable but not proven to exist at this time. The kinds of living organisms currently known on Earth all use carbon compounds for basic structural and metabolic functions, water as a solvent, and DNA or RNA to define and control their form. If life exists on other planets or moons it may be chemically similar though it is also possible that there are organisms with quite different chemistries – for instance, involving other classes of carbon compounds, compounds of another element, or another solvent in place of water.

The possibility of life-forms being based on "alternative" biochemistries is the topic of an ongoing scientific discussion, informed by what is known about extraterrestrial environments and about the chemical behaviour of various elements and compounds. It is of interest in synthetic biology and is also a common subject in science fiction.

The element silicon has been much discussed as a hypothetical alternative to carbon. Silicon is in the same group as carbon on the periodic table and, like carbon, it is tetravalent. Hypothetical alternatives to water include ammonia, which, like water, is a polar molecule, and cosmically abundant; and non-polar hydrocarbon solvents such as methane and ethane, which are known to exist in liquid form on the surface of Titan.

Overview

Overview of hypothetical types of biochemistry

Type	Basis	Synopsis	Remarks
Alternative-chirality biomolecules	Alternative biochemistry	Different basis of biofunction	Perhaps the least unusual alternative biochemistry would be one with differing chirality of its biomolecules. In known Earth-based life, amino acids are almost universally of the L form and sugars are of the D form. Molecules using D amino acids or L sugars may be possible; molecules of such a chirality, however, would be incompatible with organisms using the opposing chirality molecules.
Ammonia biochemistry	Non-water solvents	Ammonia-based life	The possible role of liquid ammonia as an alternative solvent for life is an idea that goes back at least to 1954, when J. B. S. Haldane raised the topic at a symposium about life's origin.
Arsenic biochemistry	Alternative biochemistry	Arsenic-based life	Arsenic, which is chemically similar to phosphorus, while poisonous for most life forms on Earth, is incorporated into the biochemistry of some organisms.
Borane biochemistry (Organoboron chemistry)	Alternative biochemistry	Boranes-based life	Boranes are dangerously explosive in Earth's atmosphere, but would be more stable in a reducing environment. Boron, however, is exceedingly rare in the universe in comparison to its neighbours carbon, nitrogen, and oxygen.
Dust and plasma-based biochemistry	Nonplanetary life	Exotic matrix life	In 2007, Vadim N. Tsytovich and colleagues proposed that lifelike behaviors could be exhibited by dust particles suspended in a plasma, under conditions that might exist in space.
Extremophiles	Alternative environment	Life in variable environments	It would be biochemically possible to sustain life in environments that are only periodically consistent with life as we know it.
Heteropoly acid biochemistry	Alternative biochemistry	Heteropoly acid-based life	Various metals, together with oxygen, can form very complex and thermally stable structures rivaling those of organic compounds; the heteropoly acids are one such family.
Hydrogen fluoride biochemistry	Non-water solvents	Hydrogen fluoride-based life	It has been considered as a possible solvent for life by scientists such as Peter Sneath.
Hydrogen sulfide biochemistry	Non-water solvents	Hydrogen sulfide-based life	Hydrogen sulfide is the closest chemical analog to water, but is less polar and a weaker inorganic solvent.
Methane biochemistry (Azotosome)	Non-water solvents	Methane-based life	Methane (CH4) is a simple hydrocarbon: that is, a compound of two of the most common elements in the cosmos: hydrogen and carbon. Methane life is hypothetically possible.
Non-green photosynthesizers	Other speculations	Alternate plant life	Physicists have noted that, although photosynthesis on Earth generally involves green plants, a variety of other-colored plants could also support photosynthesis, essential for most life on Earth, and that other colors might be preferred in places that receive a different mix of stellar radiation than Earth. In particular, retinal is capable of, and has been observed to, perform photosynthesis. Bacteria capable of photosynthesis are known as microbial rhodopsins. A plant or creature that uses retinal photosynthesis is always purple.
Shadow biosphere	Alternative environment	A hidden life biosphere on Earth	A shadow biosphere is a hypothetical microbial biosphere of Earth that uses radically different biochemical and molecular processes than currently known life.
Silicon biochemistry (Organosilicon)	Alternative biochemistry	Silicon-based life	Like carbon, silicon can create molecules that are sufficiently large to carry biological information; however, the scope of possible silicon chemistry is far more limited than that of carbon.
Silicon dioxide biochemistry	Non-water solvents	Silicon dioxide-based life	Gerald Feinberg and Robert Shapiro have suggested that molten silicate rock could serve as a liquid medium for organisms with a chemistry based on silicon, oxygen, and other elements such as aluminium.
Sulfur biochemistry	Alternative biochemistry	Sulfur-based life	The biological use of sulfur as an alternative to carbon is purely hypothetical, especially because sulfur usually forms only linear chains rather than branched ones.

Shadow biosphere

The Arecibo message (1974) sent information into space about basic chemistry of Earth life.

A shadow biosphere is a hypothetical microbial biosphere of Earth that uses radically different biochemical and molecular processes than currently known life. Although life on Earth is relatively well-studied, the shadow biosphere may still remain unnoticed because the exploration of the microbial world targets primarily the biochemistry of the macro-organisms.

Alternative-chirality biomolecules

Perhaps the least unusual alternative biochemistry would be one with differing chirality of its biomolecules. In known Earth-based life, amino acids are almost universally of the L form and sugars are of the D form. Molecules using D amino acids or L sugars may be possible; molecules of such a chirality, however, would be incompatible with organisms using the opposing chirality molecules. Amino acids whose chirality is opposite to the norm are found on Earth, and these substances are generally thought to result from decay of organisms of normal chirality. However, physicist Paul Davies speculates that some of them might be products of "anti-chiral" life.

It is questionable, however, whether such a biochemistry would be truly alien. Although it would certainly be an alternative stereochemistry, molecules that are overwhelmingly found in one enantiomer throughout the vast majority of organisms can nonetheless often be found in another enantiomer in different (often basal) organisms such as in comparisons between members of Archaea and other domains, making it an open topic whether an alternative stereochemistry is truly novel.

Non-carbon-based biochemistries

On Earth, all known living things have a carbon-based structure and system. Scientists have speculated about the pros and cons of using atoms other than carbon to form the molecular structures necessary for life, but no one has proposed a theory employing such atoms to form all the necessary structures. However, as Carl Sagan argued, it is very difficult to be certain whether a statement that applies to all life on Earth will turn out to apply to all life throughout the universe. Sagan used the term "carbon chauvinism" for such an assumption. He regarded silicon and germanium as conceivable alternatives to carbon (other plausible elements include but are not limited to palladium and titanium); but, on the other hand, he noted that carbon does seem more chemically versatile and is more abundant in the cosmos. Norman Horowitz devised the experiments to determine whether life might exist on Mars that were carried out by the Viking Lander of 1976, the first U.S. mission to successfully land an unmanned probe on the surface of Mars. Horowitz argued that the great versatility of the carbon atom makes it the element most likely to provide solutions, even exotic solutions, to the problems of survival on other planets. He considered that there was only a remote possibility that non-carbon life forms could exist with genetic information systems capable of self-replication and the ability to evolve and adapt.

Silicon biochemistry

Structure of silane, analog of methane

 

Structure of the silicone polydimethylsiloxane (PDMS)

 

Marine diatoms – carbon-based organisms that extract silicon from sea water, in the form of its oxide (silica) and incorporate it into their cell walls

The silicon atom has been much discussed as the basis for an alternative biochemical system, because silicon has many chemical properties similar to those of carbon and is in the same group of the periodic table, the carbon group. Like carbon, silicon can create molecules that are sufficiently large to carry biological information.

However, silicon has several drawbacks as an alternative to carbon. Silicon, unlike carbon, lacks the ability to form chemical bonds with diverse types of atoms as is necessary for the chemical versatility required for metabolism, and yet this precise inability is what makes silicon less susceptible to bond with all sorts of impurities from which carbon, in comparison, is not shielded. Elements creating organic functional groups with carbon include hydrogen, oxygen, nitrogen, phosphorus, sulfur, and metals such as iron, magnesium, and zinc. Silicon, on the other hand, interacts with very few other types of atoms. Moreover, where it does interact with other atoms, silicon creates molecules that have been described as "monotonous compared with the combinatorial universe of organic macromolecules". This is because silicon atoms are much bigger, having a larger mass and atomic radius, and so have difficulty forming double bonds (the double-bonded carbon is part of the carbonyl group, a fundamental motif of carbon-based bio-organic chemistry).

Silanes, which are chemical compounds of hydrogen and silicon that are analogous to the alkane hydrocarbons, are highly reactive with water, and long-chain silanes spontaneously decompose. Molecules incorporating polymers of alternating silicon and oxygen atoms instead of direct bonds between silicon, known collectively as silicones, are much more stable. It has been suggested that silicone-based chemicals would be more stable than equivalent hydrocarbons in a sulfuric-acid-rich environment, as is found in some extraterrestrial locations.

Of the varieties of molecules identified in the interstellar medium as of 1998, 84 are based on carbon, while only 8 are based on silicon. Moreover, of those 8 compounds, 4 also include carbon within them. The cosmic abundance of carbon to silicon is roughly 10 to 1. This may suggest a greater variety of complex carbon compounds throughout the cosmos, providing less of a foundation on which to build silicon-based biologies, at least under the conditions prevalent on the surface of planets. Also, even though Earth and other terrestrial planets are exceptionally silicon-rich and carbon-poor (the relative abundance of silicon to carbon in Earth's crust is roughly 925:1), terrestrial life is carbon-based. The fact that carbon is used instead of silicon may be evidence that silicon is poorly suited for biochemistry on Earth-like planets. Reasons for which this may be that silicon is less versatile than carbon in forming compounds, that the compounds formed by silicon are unstable, and that it blocks the flow of heat.

Even so, biogenic silica is used by some Earth life, such as the silicate skeletal structure of diatoms. According to the clay hypothesis of A. G. Cairns-Smith, silicate minerals in water played a crucial role in abiogenesis: they replicated their crystal structures, interacted with carbon compounds, and were the precursors of carbon-based life.

Although not observed in nature, carbon–silicon bonds have been added to biochemistry by using directed evolution (artificial selection). A heme containing cytochrome c protein from Rhodothermus marinus has been engineered using directed evolution to catalyze the formation of new carbon–silicon bonds between hydrosilanes and diazo compounds.

Silicon compounds may possibly be biologically useful under temperatures or pressures different from the surface of a terrestrial planet, either in conjunction with or in a role less directly analogous to carbon. Polysilanols, the silicon compounds corresponding to sugars, are soluble in liquid nitrogen, suggesting that they could play a role in very-low-temperature biochemistry.

In cinematic and literary science fiction, at a moment when man-made machines cross from nonliving to living, it is often posited, this new form would be the first example of non-carbon-based life. Since the advent of the microprocessor in the late 1960s, these machines are often classed as computers (or computer-guided robots) and filed under "silicon-based life", even though the silicon backing matrix of these processors is not nearly as fundamental to their operation as carbon is for "wet life".

Other exotic element-based biochemistries

• Boranes are dangerously explosive in Earth's atmosphere, but would be more stable in a reducing atmosphere. However, boron's low cosmic abundance makes it less likely as a base for life than carbon.
• Various metals, together with oxygen, can form very complex and thermally stable structures rivaling those of organic compounds; the heteropoly acids are one such family. Some metal oxides are also similar to carbon in their ability to form both nanotube structures and diamond-like crystals (such as cubic zirconia). Titanium, aluminium, magnesium, and iron are all more abundant in the Earth's crust than carbon. Metal-oxide-based life could therefore be a possibility under certain conditions, including those (such as high temperatures) at which carbon-based life would be unlikely. The Cronin group at Glasgow University reported self-assembly of tungsten polyoxometalates into cell-like spheres. By modifying their metal oxide content, the spheres can acquire holes that act as porous membrane, selectively allowing chemicals in and out of the sphere according to size.
• Sulfur is also able to form long-chain molecules, but suffers from the same high-reactivity problems as phosphorus and silanes. The biological use of sulfur as an alternative to carbon is purely hypothetical, especially because sulfur usually forms only linear chains rather than branched ones. (The biological use of sulfur as an electron acceptor is widespread and can be traced back 3.5 billion years on Earth, thus predating the use of molecular oxygen. Sulfur-reducing bacteria can utilize elemental sulfur instead of oxygen, reducing sulfur to hydrogen sulfide.)

Arsenic as an alternative to phosphorus

Arsenic, which is chemically similar to phosphorus, while poisonous for most life forms on Earth, is incorporated into the biochemistry of some organisms. Some marine algae incorporate arsenic into complex organic molecules such as arsenosugars and arsenobetaines. Fungi and bacteria can produce volatile methylated arsenic compounds. Arsenate reduction and arsenite oxidation have been observed in microbes (Chrysiogenes arsenatis). Additionally, some prokaryotes can use arsenate as a terminal electron acceptor during anaerobic growth and some can utilize arsenite as an electron donor to generate energy.

It has been speculated that the earliest life forms on Earth may have used arsenic biochemistry in place of phosphorus in the structure of their DNA. A common objection to this scenario is that arsenate esters are so much less stable to hydrolysis than corresponding phosphate esters that arsenic is poorly suited for this function.

The authors of a 2010 geomicrobiology study, supported in part by NASA, have postulated that a bacterium, named GFAJ-1, collected in the sediments of Mono Lake in eastern California, can employ such 'arsenic DNA' when cultured without phosphorus. They proposed that the bacterium may employ high levels of poly-β-hydroxybutyrate or other means to reduce the effective concentration of water and stabilize its arsenate esters. This claim was heavily criticized almost immediately after publication for the perceived lack of appropriate controls. Science writer Carl Zimmer contacted several scientists for an assessment: "I reached out to a dozen experts ... Almost unanimously, they think the NASA scientists have failed to make their case". Other authors were unable to reproduce their results and showed that the study had issues with phosphate contamination, suggesting that the low amounts present could sustain extremophile lifeforms. Alternatively, it was suggested that GFAJ-1 cells grow by recycling phosphate from degraded ribosomes, rather than by replacing it with arsenate.

Non-water solvents

In addition to carbon compounds, all currently known terrestrial life also requires water as a solvent. This has led to discussions about whether water is the only liquid capable of filling that role. The idea that an extraterrestrial life-form might be based on a solvent other than water has been taken seriously in recent scientific literature by the biochemist Steven Benner, and by the astrobiological committee chaired by John A. Baross. Solvents discussed by the Baross committee include ammonia, sulfuric acid, formamide, hydrocarbons, and (at temperatures much lower than Earth's) liquid nitrogen, or hydrogen in the form of a supercritical fluid.

Carl Sagan once described himself as both a carbon chauvinist and a water chauvinist; however, on another occasion he said that he was a carbon chauvinist but "not that much of a water chauvinist". He speculated on hydrocarbons, hydrofluoric acid, and ammonia as possible alternatives to water.

Some of the properties of water that are important for life processes include:

• A complexity which leads to a large number of permutations of possible reaction paths including acid–base chemistry, H⁺ cations, OH⁻ anions, hydrogen bonding, van der Waals bonding, dipole–dipole and other polar interactions, aqueous solvent cages, and hydrolysis. This complexity offers a large number of pathways for evolution to produce life, many other solvents have dramatically fewer possible reactions, which severely limits evolution.
• Thermodynamic stability: the free energy of formation of liquid water is low enough (−237.24 kJ/mol) that water undergoes few reactions. Other solvents are highly reactive, particularly with oxygen.
• Water does not combust in oxygen because it is already the combustion product of hydrogen with oxygen. Most alternative solvents are not stable in an oxygen-rich atmosphere, so it is highly unlikely that those liquids could support aerobic life.
• A large temperature range over which it is liquid.
• High solubility of oxygen and carbon dioxide at room temperature supporting the evolution of aerobic aquatic plant and animal life.
• A high heat capacity (leading to higher environmental temperature stability).
• Water is a room-temperature liquid leading to a large population of quantum transition states required to overcome reaction barriers. Cryogenic liquids (such as liquid methane) have exponentially lower transition state populations which are needed for life based on chemical reactions. This leads to chemical reaction rates which may be so slow as to preclude the development of any life based on chemical reactions.
• Spectroscopic transparency allowing solar radiation to penetrate several meters into the liquid (or solid), greatly aiding the evolution of aquatic life.
• A large heat of vaporization leading to stable lakes and oceans.
• The ability to dissolve a wide variety of compounds.
• The solid (ice) has lower density than the liquid, so ice floats on the liquid. This is why bodies of water freeze over but do not freeze solid (from the bottom up). If ice were denser than liquid water (as is true for nearly all other compounds), then large bodies of liquid would slowly freeze solid, which would not be conducive to the formation of life.

Water as a compound is cosmically abundant, although much of it is in the form of vapour or ice. Subsurface liquid water is considered likely or possible on several of the outer moons: Enceladus (where geysers have been observed), Europa, Titan, and Ganymede. Earth and Titan are the only worlds currently known to have stable bodies of liquid on their surfaces.

Not all properties of water are necessarily advantageous for life, however. For instance, water ice has a high albedo, meaning that it reflects a significant quantity of light and heat from the Sun. During ice ages, as reflective ice builds up over the surface of the water, the effects of global cooling are increased.

There are some properties that make certain compounds and elements much more favorable than others as solvents in a successful biosphere. The solvent must be able to exist in liquid equilibrium over a range of temperatures the planetary object would normally encounter. Because boiling points vary with the pressure, the question tends not to be does the prospective solvent remain liquid, but at what pressure. For example, hydrogen cyanide has a narrow liquid-phase temperature range at 1 atmosphere, but in an atmosphere with the pressure of Venus, with 92 bars (91 atm) of pressure, it can indeed exist in liquid form over a wide temperature range.

Ammonia

Artist's conception of how a planet with ammonia-based life might look

The ammonia molecule (NH₃), like the water molecule, is abundant in the universe, being a compound of hydrogen (the simplest and most common element) with another very common element, nitrogen. The possible role of liquid ammonia as an alternative solvent for life is an idea that goes back at least to 1954, when J. B. S. Haldane raised the topic at a symposium about life's origin.

Numerous chemical reactions are possible in an ammonia solution, and liquid ammonia has chemical similarities with water. Ammonia can dissolve most organic molecules at least as well as water does and, in addition, it is capable of dissolving many elemental metals. Haldane made the point that various common water-related organic compounds have ammonia-related analogs; for instance the ammonia-related amine group (−NH₂) is analogous to the water-related hydroxyl group (−OH).

Ammonia, like water, can either accept or donate an H⁺ ion. When ammonia accepts an H⁺, it forms the ammonium cation (NH₄⁺), analogous to hydronium (H₃O⁺). When it donates an H⁺ ion, it forms the amide anion (NH₂⁻), analogous to the hydroxide anion (OH⁻). Compared to water, however, ammonia is more inclined to accept an H⁺ ion, and less inclined to donate one; it is a stronger nucleophile. Ammonia added to water functions as Arrhenius base: it increases the concentration of the anion hydroxide. Conversely, using a solvent system definition of acidity and basicity, water added to liquid ammonia functions as an acid, because it increases the concentration of the cation ammonium. The carbonyl group (C=O), which is much used in terrestrial biochemistry, would not be stable in ammonia solution, but the analogous imine group (C=NH) could be used instead.

However, ammonia has some problems as a basis for life. The hydrogen bonds between ammonia molecules are weaker than those in water, causing ammonia's heat of vaporization to be half that of water, its surface tension to be a third, and reducing its ability to concentrate non-polar molecules through a hydrophobic effect. Gerald Feinberg and Robert Shapiro have questioned whether ammonia could hold prebiotic molecules together well enough to allow the emergence of a self-reproducing system. Ammonia is also flammable in oxygen and could not exist sustainably in an environment suitable for aerobic metabolism.

Titan's theorized internal structure, subsurface ocean shown in blue

A biosphere based on ammonia would likely exist at temperatures or air pressures that are extremely unusual in relation to life on Earth. Life on Earth usually exists within the melting point and boiling point of water at normal pressure, between 0 °C (273 K) and 100 °C (373 K); at normal pressure ammonia's melting and boiling points are between −78 °C (195 K) and −33 °C (240 K). Chemical reactions generally proceed more slowly at a lower temperature. Therefore, ammonia-based life, if it exists, might metabolize more slowly and evolve more slowly than life on Earth. On the other hand, lower temperatures could also enable living systems to use chemical species that would be too unstable at Earth temperatures to be useful.

Ammonia could be a liquid at Earth-like temperatures, but at much higher pressures; for example, at 60 atm, ammonia melts at −77 °C (196 K) and boils at 98 °C (371 K).

Ammonia and ammonia–water mixtures remain liquid at temperatures far below the freezing point of pure water, so such biochemistries might be well suited to planets and moons orbiting outside the water-based habitability zone. Such conditions could exist, for example, under the surface of Saturn's largest moon Titan.

Methane and other hydrocarbons

Methane (CH₄) is a simple hydrocarbon: that is, a compound of two of the most common elements in the cosmos: hydrogen and carbon. It has a cosmic abundance comparable with ammonia. Hydrocarbons could act as a solvent over a wide range of temperatures, but would lack polarity. Isaac Asimov, the biochemist and science fiction writer, suggested in 1981 that poly-lipids could form a substitute for proteins in a non-polar solvent such as methane. Lakes composed of a mixture of hydrocarbons, including methane and ethane, have been detected on the surface of Titan by the Cassini spacecraft.

There is debate about the effectiveness of methane and other hydrocarbons as a solvent for life compared to water or ammonia. Water is a stronger solvent than the hydrocarbons, enabling easier transport of substances in a cell. However, water is also more chemically reactive and can break down large organic molecules through hydrolysis. A life-form whose solvent was a hydrocarbon would not face the threat of its biomolecules being destroyed in this way. Also, the water molecule's tendency to form strong hydrogen bonds can interfere with internal hydrogen bonding in complex organic molecules. Life with a hydrocarbon solvent could make more use of hydrogen bonds within its biomolecules. Moreover, the strength of hydrogen bonds within biomolecules would be appropriate to a low-temperature biochemistry.

Astrobiologist Chris McKay has argued, on thermodynamic grounds, that if life does exist on Titan's surface, using hydrocarbons as a solvent, it is likely also to use the more complex hydrocarbons as an energy source by reacting them with hydrogen, reducing ethane and acetylene to methane. Possible evidence for this form of life on Titan was identified in 2010 by Darrell Strobel of Johns Hopkins University; a greater abundance of molecular hydrogen in the upper atmospheric layers of Titan compared to the lower layers, arguing for a downward diffusion at a rate of roughly 10²⁵ molecules per second and disappearance of hydrogen near Titan's surface. As Strobel noted, his findings were in line with the effects Chris McKay had predicted if methanogenic life-forms were present. The same year, another study showed low levels of acetylene on Titan's surface, which were interpreted by Chris McKay as consistent with the hypothesis of organisms reducing acetylene to methane. While restating the biological hypothesis, McKay cautioned that other explanations for the hydrogen and acetylene findings are to be considered more likely: the possibilities of yet unidentified physical or chemical processes (e.g. a non-living surface catalyst enabling acetylene to react with hydrogen), or flaws in the current models of material flow. He noted that even a non-biological catalyst effective at 95 K would in itself be a startling discovery.

Azotosome

A hypothetical cell membrane termed an azotosome capable of functioning in liquid methane in Titan conditions was computer-modeled in an article published in February 2015. Composed of acrylonitrile, a small molecule containing carbon, hydrogen, and nitrogen, it is predicted to have stability and flexibility in liquid methane comparable to that of a phospholipid bilayer (the type of cell membrane possessed by all life on Earth) in liquid water. An analysis of data obtained using the Atacama Large Millimeter / submillimeter Array (ALMA), completed in 2017, confirmed substantial amounts of acrylonitrile in Titan's atmosphere.

Hydrogen fluoride

Hydrogen fluoride (HF), like water, is a polar molecule, and due to its polarity it can dissolve many ionic compounds. Its melting point is −84 °C, and its boiling point is 19.54 °C (at atmospheric pressure); the difference between the two is a little more than 100 K. HF also makes hydrogen bonds with its neighbor molecules, as do water and ammonia. It has been considered as a possible solvent for life by scientists such as Peter Sneath and Carl Sagan.

HF is dangerous to the systems of molecules that Earth-life is made of, but certain other organic compounds, such as paraffin waxes, are stable with it. Like water and ammonia, liquid hydrogen fluoride supports an acid–base chemistry. Using a solvent system definition of acidity and basicity, nitric acid functions as a base when it is added to liquid HF.

However, hydrogen fluoride is cosmically rare, unlike water, ammonia, and methane.

Hydrogen sulfide

Hydrogen sulfide is the closest chemical analog to water, but is less polar and a weaker inorganic solvent. Hydrogen sulfide is quite plentiful on Jupiter's moon Io and may be in liquid form a short distance below the surface; astrobiologist Dirk Schulze-Makuch has suggested it as a possible solvent for life there. On a planet with hydrogen-sulfide oceans the source of the hydrogen sulfide could come from volcanos, in which case it could be mixed in with a bit of hydrogen fluoride, which could help dissolve minerals. Hydrogen-sulfide life might use a mixture of carbon monoxide and carbon dioxide as their carbon source. They might produce and live on sulfur monoxide, which is analogous to oxygen (O₂). Hydrogen sulfide, like hydrogen cyanide and ammonia, suffers from the small temperature range where it is liquid, though that, like that of hydrogen cyanide and ammonia, increases with increasing pressure.

Silicon dioxide and silicates

Silicon dioxide, also known as silica and quartz, is very abundant in the universe and has a large temperature range where it is liquid. However, its melting point is 1,600 to 1,725 °C (2,912 to 3,137 °F), so it would be impossible to make organic compounds in that temperature, because all of them would decompose. Silicates are similar to silicon dioxide and some have lower melting points than silica. Gerald Feinberg and Robert Shapiro have suggested that molten silicate rock could serve as a liquid medium for organisms with a chemistry based on silicon, oxygen, and other elements such as aluminium.

Other solvents or cosolvents

Sulfuric acid (H₂SO₄)

Other solvents sometimes proposed:

• Supercritical fluids: supercritical carbon dioxide and supercritical hydrogen.
• Simple hydrogen compounds: hydrogen chloride.
• More complex compounds: sulfuric acid, formamide, methanol.
• Very-low-temperature fluids: liquid nitrogen and hydrogen.
• High-temperature liquids: sodium chloride.

Sulfuric acid in liquid form is strongly polar. It remains liquid at higher temperatures than water, its liquid range being 10 °C to 337 °C at a pressure of 1 atm, although above 300 °C it slowly decomposes. Sulfuric acid is known to be abundant in the clouds of Venus, in the form of aerosol droplets. In a biochemistry that used sulfuric acid as a solvent, the alkene group (C=C), with two carbon atoms joined by a double bond, could function analogously to the carbonyl group (C=O) in water-based biochemistry.

A proposal has been made that life on Mars may exist and be using a mixture of water and hydrogen peroxide as its solvent. A 61.2% (by mass) mix of water and hydrogen peroxide has a freezing point of −56.5 °C and tends to super-cool rather than crystallize. It is also hygroscopic, an advantage in a water-scarce environment.

Supercritical carbon dioxide has been proposed as a candidate for alternative biochemistry due to its ability to selectively dissolve organic compounds and assist the functioning of enzymes and because "super-Earth"- or "super-Venus"-type planets with dense high-pressure atmospheres may be common.

Other speculations

Non-green photosynthesizers

Physicists have noted that, although photosynthesis on Earth generally involves green plants, a variety of other-colored plants could also support photosynthesis, essential for most life on Earth, and that other colors might be preferred in places that receive a different mix of stellar radiation than Earth. These studies indicate that blue plants would be unlikely; however yellow or red plants may be relatively common.

Variable environments

Many Earth plants and animals undergo major biochemical changes during their life cycles as a response to changing environmental conditions, for example, by having a spore or hibernation state that can be sustained for years or even millennia between more active life stages. Thus, it would be biochemically possible to sustain life in environments that are only periodically consistent with life as we know it.

For example, frogs in cold climates can survive for extended periods of time with most of their body water in a frozen state, whereas desert frogs in Australia can become inactive and dehydrate in dry periods, losing up to 75% of their fluids, yet return to life by rapidly rehydrating in wet periods. Either type of frog would appear biochemically inactive (i.e. not living) during dormant periods to anyone lacking a sensitive means of detecting low levels of metabolism.

Alanine world and hypothetical alternatives

Early stage of the genetic code (GC-Code) with "alanine world" and its possible alternatives.

The genetic code evolved during the transition from the RNA world to a protein world. The Alanine World Hypothesis postulates that the evolution of the genetic code (the so-called GC phase) started with only four basic amino acids: alanine, glycine, proline and ornithine (now arginine). The evolution of the genetic code ended with 20 proteinogenic amino acids. From a chemical point of view, most of them are Alanine-derivatives particularly suitable for the construction of α-helices and β-sheets – basic secondary structural elements of modern proteins. Direct evidence of this is an experimental procedure in molecular biology known as alanine scanning. The hypothetical "Proline World" would create a possible alternative life with the genetic code based on the proline chemical scaffold as the protein backbone. Similarly, "Glycine" and "Ornithine" worlds are also conceivable, but nature has chosen none of them. Evolution of life with Glycine, Proline or Ornithine as the basic structure for protein-like polymers (foldamers) would lead to parallel biological worlds. They would have morphologically radically different body plans and genetics from the living organisms of the known biosphere.

Nonplanetary life

Dust and plasma-based

In 2007, Vadim N. Tsytovich and colleagues proposed that lifelike behaviors could be exhibited by dust particles suspended in a plasma, under conditions that might exist in space. Computer models showed that, when the dust became charged, the particles could self-organize into microscopic helical structures, and the authors offer "a rough sketch of a possible model of...helical grain structure reproduction".

Life on a Neutron Star

Frank Drake suggested in 1973 that intelligent life could inhabit neutron stars. Physical models in 1973 implied that Drake's creatures would be microscopic. In 1980, Robert L Forward wrote the science fiction novel Dragon's Egg using Drake's suggestion as a thesis.

Astroecology

From Wikipedia, the free encyclopedia

https://en.wikipedia.org/wiki/Astroecology 

Astroecology concerns the interactions of biota with space environments. It studies resources for life on planets, asteroids and comets, around various stars, in galaxies, and in the universe. The results allow estimating the future prospects for life, from planetary to galactic and cosmological scales.

Available energy, and microgravity, radiation, pressure and temperature are physical factors that affect astroecology. The ways by which life can reach space environments, including natural panspermia and directed panspermia are also considered. Further, for human expansion in space and directed panspermia, motivation by life-centered biotic ethics, panbiotic ethics and planetary bioethics are also relevant.

Overview

The term "astroecology" was first applied in the context of performing studies in actual meteorites to evaluate their potential resources favorable to sustaining life. Early results showed that meteorite/asteroid materials can support microorganisms, algae and plant cultures under Earth's atmosphere and supplemented with water.

Several observations suggest that diverse planetary materials, similar to meteorites collected on Earth, could be used as agricultural soils, as they provide nutrients to support microscopic life when supplemented with water and an atmosphere. Experimental astroecology has been proposed to rate planetary materials as targets for astrobiology exploration and as potential biological in-situ resources. The biological fertilities of planetary materials can be assessed by measuring water-extractable electrolyte nutrients. The results suggest that carbonaceous asteroids and Martian basalts can serve as potential future resources for substantial biological populations in the Solar System.

Analysis of the essential nutrients (C, N, P, K) in meteorites yielded information for calculating the amount of biomass that can be constructed from asteroid resources. For example, carbonaceous asteroids are estimated to contain about 10²² kg potential resource materials, and laboratory results suggest that they could yield a biomass on the order of 6·10²⁰ kg, about 100,000 times more than biological matter presently on Earth.

Cultures on simulated asteroid/meteorite materials

To quantify the potential amounts of life in biospheres, theoretical astroecology attempts to estimate the amount of biomass over the duration of a biosphere. The resources, and the potential time-integrated biomass were estimated for planetary systems, for habitable zones around stars, and for the galaxy and the universe. Such astroecology calculations suggest that the limiting elements nitrogen and phosphorus in the estimated 10²² kg carbonaceous asteroids could support 6·10²⁰ kg biomass for the expected five billion future years of the Sun, yielding a future time-integrated BIOTA (BIOTA, Biomass Integrated Over Times Available, measured in kilogram-years) of 3·10³⁰ kg-years in the Solar System a hundred thousand times more than life on Earth to date. Considering biological requirements of 100 W kg⁻¹ biomass, radiated energy about red giant stars and white and red dwarf stars could support a time-integrated BIOTA up to 10⁴⁶ kg-years in the galaxy and 10⁵⁷ kg-years in the universe.

Such astroecology considerations quantify the immense potentials of future life in space, with commensurate biodiversity and possibly, intelligence. Chemical analysis of carbonaceous chondrite meteorites show that they contain extractable bioavailable water, organic carbon, and essential phosphate, nitrate and potassium nutrients. The results allow assessing the soil fertilities of the parent asteroids and planets, and the amounts of biomass that they can sustain.

Laboratory experiments showed that material from the Murchison meteorite, when ground into a fine powder and combined with Earth's water and air, can provide the nutrients to support a variety of organisms including bacteria (Nocardia asteroides), algae, and plant cultures such as potato and asparagus. The microorganisms used organics in the carbonaceous meteorites as the carbon source. Algae and plant cultures grew well also on Mars meteorites because of their high bio-available phosphate contents. The Martian materials achieved soil fertility ratings comparable to productive agricultural soils. This offers some data relating to terraforming of Mars.

Terrestrial analogues of planetary materials are also used in such experiments for comparison, and to test the effects of space conditions on microorganisms.

The biomass that can be constructed from resources can be calculated by comparing the concentration of elements in the resource materials and in biomass (Equation 1). A given mass of resource materials (m_resource) can support m_{biomass, X} of biomass containing element X (considering X as the limiting nutrient), where c_{resource, X} is the concentration (mass per unit mass) of element X in the resource material and c_{biomass, X} is its concentration in the biomass.

${\displaystyle m_{biomass,\,X}=m_{resource,\,X}{\frac {c_{resource,\,X}}{c_{biomass,\,X}}}}$ (1)

Assuming that 100,000 kg biomass supports one human, the asteroids may then sustain about 6e15 (six million billion) people, equal to a million Earths (a million times the present population). Similar materials in the comets could support biomass and populations about one hundred times larger. Solar energy can sustain these populations for the predicted further five billion years of the Sun. These considerations yield a maximum time-integrated BIOTA of 3e30 kg-years in the Solar System. After the Sun becomes a white dwarf star, and other white dwarf stars, can provide energy for life much longer, for trillions of eons. (Table 2)

Effects of wastage

Astroecology also concerns wastage, such as the leakage of biological matter into space. This will cause an exponential decay of space-based biomass as given by Equation (2), where M (biomass 0) is the mass of the original biomass, k is its rate of decay (the fraction lost in a unit time) and biomass t is the remaining biomass after time t.

Equation 2: ${\displaystyle M_{biomass}(t)=M_{biomass}(0)e^{-kt}\,}$

Integration from time zero to infinity yields Equation (3) for the total time-integrated biomass (BIOTA) contributed by this biomass:

Equation 3: ${\displaystyle BIOTA={\frac {M_{biomass}(0)}{k}}}$

For example, if 0.01% of the biomass is lost per year, then the time-integrated BIOTA will be 10,000${\displaystyle M_{biomass}(0)}$. For the 6·10²⁰ kg biomass constructed from asteroid resources, this yields 6·10²⁴ kg-years of BIOTA in the Solar System. Even with this small rate of loss, life in the Solar System would disappear in a few hundred thousand years, and the potential total time-integrated BIOTA of 3·10³⁰ kg-years under the main-sequence Sun would decrease by a factor of 5·10⁵, although a still substantial population of 1.2·10¹² biomass-supported humans could exist through the habitable lifespan of the Sun. The integrated biomass can be maximized by minimizing its rate of dissipation. If this rate can be reduced sufficiently, all the constructed biomass can last for the duration of the habitat and it pays to construct the biomass as fast as possible. However, if the rate of dissipation is significant, the construction rate of the biomass and its steady-state amounts may be reduced allowing a steady-state biomass and population that lasts throughout the lifetime of the habitat.

An issue that arises is whether we should build immense amounts of life that decays fast, or smaller, but still large, populations that last longer. Life-centered biotic ethics suggests that life should last as long as possible.

Galactic ecology

If life reaches galactic proportions, technology should be able to access all of the materials resources, and sustainable life will be defined by the available energy. The maximum amount of biomass about any star is then determined by the energy requirements of the biomass and by the luminosity of the star. For example, if 1 kg biomass needs 100 Watts, we can calculate the steady-state amounts of biomass that can be sustained by stars with various energy outputs. These amounts are multiplied by the life-time of the star to calculate the time-integrated BIOTA over the life-time of the star. Using similar projections, the potential amounts of future life can then be quantified.

For the Solar System from its origins to the present, the current 10¹⁵ kg biomass over the past four billion years gives a time-integrated biomass (BIOTA) of 4·10²⁴ kg-years. In comparison, carbon, nitrogen, phosphorus and water in the 10²² kg asteroids allows 6·10²⁰ kg biomass that can be sustained with energy for the 5 billion future years of the Sun, giving a BIOTA of 3·10³⁰ kg-years in the Solar System and 3·10³⁹ kg-years about 10¹¹ stars in the galaxy. Materials in comets could give biomass and time-integrated BIOTA a hundred times larger.

The Sun will then become a white dwarf star, radiating 10¹⁵ Watts that sustains 1e13 kg biomass for an immense hundred million trillion (10²⁰) years, contributing a time-integrated BIOTA of 10³³ years. The 10¹² white dwarfs that may exist in the galaxy during this time can then contribute a time-integrated BIOTA of 10⁴⁵ kg-years. Red dwarf stars with luminosities of 10²³ Watts and life-times of 10¹³ years can contribute 10³⁴ kg-years each, and 10¹² red dwarfs can contribute 10⁴⁶ kg-years, while brown dwarfs can contribute 10³⁹ kg-years of time-integrated biomass (BIOTA) in the galaxy. In total, the energy output of stars during 10²⁰ years can sustain a time-integrated biomass of about 10⁴⁵ kg-years in the galaxy. This is one billion trillion (10²⁰) times more life than has existed on the Earth to date. In the universe, stars in 10¹¹ galaxies could then sustain 10⁵⁷ kg-years of life.

Directed panspermia

The astroecology results above suggest that humans can expand life in the galaxy through space travel or directed panspermia. The amounts of possible life that can be established in the galaxy, as projected by astroecology, are immense. These projections are based on information about 15 billion past years since the Big Bang, but the habitable future is much longer, spanning trillions of eons. Therefore, physics, astroeclogy resources, and some cosmological scenarios may allow organized life to last, albeit at an ever slowing rate, indefinitely. These prospects may be addressed by the long-term extension of astroecology as cosmoecology.

Astrobiology

From Wikipedia, the free encyclopedia

https://en.wikipedia.org/wiki/Astrobiology 

Nucleic acids may not be the only biomolecules in the Universe capable of coding for life processes.

Astrobiology, formerly known as exobiology, is an interdisciplinary scientific field concerned with the origins, early evolution, distribution, and future of life in the universe. Astrobiology considers the question of whether extraterrestrial life exists, and if it does, how humans can detect it.

Astrobiology makes use of molecular biology, biophysics, biochemistry, chemistry, astronomy, physical cosmology, exoplanetology and geology to investigate the possibility of life on other worlds and help recognize biospheres that might be different from that on Earth. The origin and early evolution of life is an inseparable part of the discipline of astrobiology. Astrobiology concerns itself with interpretation of existing scientific data, and although speculation is entertained to give context, astrobiology concerns itself primarily with hypotheses that fit firmly into existing scientific theories.

This interdisciplinary field encompasses research on the origin of planetary systems, origins of organic compounds in space, rock-water-carbon interactions, abiogenesis on Earth, planetary habitability, research on biosignatures for life detection, and studies on the potential for life to adapt to challenges on Earth and in outer space.

Biochemistry may have begun shortly after the Big Bang, 13.8 billion years ago, during a habitable epoch when the Universe was only 10–17 million years old. According to the panspermia hypothesis, microscopic life—distributed by meteoroids, asteroids and other small Solar System bodies—may exist throughout the universe. According to research published in August 2015, very large galaxies may be more favorable to the creation and development of habitable planets than such smaller galaxies as the Milky Way. Nonetheless, Earth is the only place in the universe humans know to harbor life. Estimates of habitable zones around other stars, sometimes referred to as "Goldilocks zones," along with the discovery of hundreds of extrasolar planets and new insights into extreme habitats here on Earth, suggest that there may be many more habitable places in the universe than considered possible until very recently.

Current studies on the planet Mars by the Curiosity and Opportunity Perseverance rovers are searching for evidence of ancient life as well as plains related to ancient rivers or lakes that may have been habitable. The search for evidence of habitability, taphonomy (related to fossils), and organic molecules on the planet Mars is now a primary NASA and ESA objective.

Even if extraterrestrial life is never discovered, the interdisciplinary nature of astrobiology, and the cosmic and evolutionary perspectives engendered by it, may still result in a range of benefits here on Earth.

Overview

The term was first proposed by the Russian (Soviet) astronomer Gavriil Tikhov in 1953. Astrobiology is etymologically derived from the Greek ἄστρον, astron, "constellation, star"; βίος, bios, "life"; and -λογία, -logia, study. The synonyms of astrobiology are diverse; however, the synonyms were structured in relation to the most important sciences implied in its development: astronomy and biology. A close synonym is exobiology from the Greek Έξω, "external"; Βίος, bios, "life"; and λογία, -logia, study. The term exobiology was coined by molecular biologist and Nobel Prize winner Joshua Lederberg. Exobiology is considered to have a narrow scope limited to search of life external to Earth, whereas subject area of astrobiology is wider and investigates the link between life and the universe, which includes the search for extraterrestrial life, but also includes the study of life on Earth, its origin, evolution and limits.

It is not known whether life elsewhere in the universe would utilize cell structures like those found on Earth. (Chloroplasts within plant cells shown here.)

Another term used in the past is xenobiology, ("biology of the foreigners") a word used in 1954 by science fiction writer Robert Heinlein in his work The Star Beast. The term xenobiology is now used in a more specialized sense, to mean "biology based on foreign chemistry", whether of extraterrestrial or terrestrial (possibly synthetic) origin. Since alternate chemistry analogs to some life-processes have been created in the laboratory, xenobiology is now considered as an extant subject.

While it is an emerging and developing field, the question of whether life exists elsewhere in the universe is a verifiable hypothesis and thus a valid line of scientific inquiry. Though once considered outside the mainstream of scientific inquiry, astrobiology has become a formalized field of study. Planetary scientist David Grinspoon calls astrobiology a field of natural philosophy, grounding speculation on the unknown, in known scientific theory. NASA's interest in exobiology first began with the development of the U.S. Space Program. In 1959, NASA funded its first exobiology project, and in 1960, NASA founded an Exobiology Program, which is now one of four main elements of NASA's current Astrobiology Program. In 1971, NASA funded the search for extraterrestrial intelligence (SETI) to search radio frequencies of the electromagnetic spectrum for interstellar communications transmitted by extraterrestrial life outside the Solar System. NASA's Viking missions to Mars, launched in 1976, included three biology experiments designed to look for metabolism of present life on Mars.

In June 2014, the John W. Kluge Center of the Library of Congress held a seminar focusing on astrobiology. Panel members (L to R) Robin Lovin, Derek Malone-France, and Steven J. Dick

Advancements in the fields of astrobiology, observational astronomy and discovery of large varieties of extremophiles with extraordinary capability to thrive in the harshest environments on Earth, have led to speculation that life may possibly be thriving on many of the extraterrestrial bodies in the universe. A particular focus of current astrobiology research is the search for life on Mars due to this planet's proximity to Earth and geological history. There is a growing body of evidence to suggest that Mars has previously had a considerable amount of water on its surface, water being considered an essential precursor to the development of carbon-based life.

Missions specifically designed to search for current life on Mars were the Viking program and Beagle 2 probes. The Viking results were inconclusive, and Beagle 2 failed minutes after landing. A future mission with a strong astrobiology role would have been the Jupiter Icy Moons Orbiter, designed to study the frozen moons of Jupiter—some of which may have liquid water—had it not been cancelled. In late 2008, the Phoenix lander probed the environment for past and present planetary habitability of microbial life on Mars, and researched the history of water there.

The European Space Agency's astrobiology roadmap from 2016, identified five main research topics, and specifies several key scientific objectives for each topic. The five research topics are: 1) Origin and evolution of planetary systems; 2) Origins of organic compounds in space; 3) Rock-water-carbon interactions, organic synthesis on Earth, and steps to life; 4) Life and habitability; 5) Biosignatures as facilitating life detection.

In November 2011, NASA launched the Mars Science Laboratory mission carrying the Curiosity rover, which landed on Mars at Gale Crater in August 2012. The Curiosity rover is currently probing the environment for past and present planetary habitability of microbial life on Mars. On 9 December 2013, NASA reported that, based on evidence from Curiosity studying Aeolis Palus, Gale Crater contained an ancient freshwater lake which could have been a hospitable environment for microbial life.

The European Space Agency is currently collaborating with the Russian Federal Space Agency (Roscosmos) and developing the ExoMars astrobiology rover, which was scheduled to be launched in July 2020, but was postponed to 2022. Meanwhile, NASA launched the Mars 2020 astrobiology rover and sample cacher for a later return to Earth.

Methodology

Planetary habitability

When looking for life on other planets like Earth, some simplifying assumptions are useful to reduce the size of the task of the astrobiologist. One is the informed assumption that the vast majority of life forms in our galaxy are based on carbon chemistries, as are all life forms on Earth. Carbon is well known for the unusually wide variety of molecules that can be formed around it. Carbon is the fourth most abundant element in the universe and the energy required to make or break a bond is at just the appropriate level for building molecules which are not only stable, but also reactive. The fact that carbon atoms bond readily to other carbon atoms allows for the building of extremely long and complex molecules.

The presence of liquid water is an assumed requirement, as it is a common molecule and provides an excellent environment for the formation of complicated carbon-based molecules that could eventually lead to the emergence of life. Some researchers posit environments of water-ammonia mixtures as possible solvents for hypothetical types of biochemistry.

A third assumption is to focus on planets orbiting Sun-like stars for increased probabilities of planetary habitability. Very large stars have relatively short lifetimes, meaning that life might not have time to emerge on planets orbiting them. Very small stars provide so little heat and warmth that only planets in very close orbits around them would not be frozen solid, and in such close orbits these planets would be tidally "locked" to the star. The long lifetimes of red dwarfs could allow the development of habitable environments on planets with thick atmospheres. This is significant, as red dwarfs are extremely common.

Since Earth is the only planet known to harbor life, there is no evident way to know if any of these simplifying assumptions are correct.

Communication attempts

The illustration on the Pioneer plaque

Research on communication with extraterrestrial intelligence (CETI) focuses on composing and deciphering messages that could theoretically be understood by another technological civilization. Communication attempts by humans have included broadcasting mathematical languages, pictorial systems such as the Arecibo message and computational approaches to detecting and deciphering 'natural' language communication. The SETI program, for example, uses both radio telescopes and optical telescopes to search for deliberate signals from an extraterrestrial intelligence.

While some high-profile scientists, such as Carl Sagan, have advocated the transmission of messages, scientist Stephen Hawking warned against it, suggesting that aliens might simply raid Earth for its resources and then move on.

Elements of astrobiology

Astronomy

Artist's impression of the extrasolar planet OGLE-2005-BLG-390Lb orbiting its star 20,000 light-years from Earth; this planet was discovered with gravitational microlensing.

 

The NASA Kepler mission, launched in March 2009, searches for extrasolar planets.

Most astronomy-related astrobiology research falls into the category of extrasolar planet (exoplanet) detection, the hypothesis being that if life arose on Earth, then it could also arise on other planets with similar characteristics. To that end, a number of instruments designed to detect Earth-sized exoplanets have been considered, most notably NASA's Terrestrial Planet Finder (TPF) and ESA's Darwin programs, both of which have been cancelled. NASA launched the Kepler mission in March 2009, and the French Space Agency launched the COROT space mission in 2006. There are also several less ambitious ground-based efforts underway.

The goal of these missions is not only to detect Earth-sized planets but also to directly detect light from the planet so that it may be studied spectroscopically. By examining planetary spectra, it would be possible to determine the basic composition of an extrasolar planet's atmosphere and/or surface. Given this knowledge, it may be possible to assess the likelihood of life being found on that planet. A NASA research group, the Virtual Planet Laboratory, is using computer modeling to generate a wide variety of virtual planets to see what they would look like if viewed by TPF or Darwin. It is hoped that once these missions come online, their spectra can be cross-checked with these virtual planetary spectra for features that might indicate the presence of life.

An estimate for the number of planets with intelligent communicative extraterrestrial life can be gleaned from the Drake equation, essentially an equation expressing the probability of intelligent life as the product of factors such as the fraction of planets that might be habitable and the fraction of planets on which life might arise:

${\displaystyle N=R^{*}~\times ~f_{p}~\times ~n_{e}~\times ~f_{l}~\times ~f_{i}~\times ~f_{c}~\times ~L}$

where:

• N = The number of communicative civilizations
• R* = The rate of formation of suitable stars (stars such as our Sun)
• f_p = The fraction of those stars with planets (current evidence indicates that planetary systems may be common for stars like the Sun)
• n_e = The number of Earth-sized worlds per planetary system
• f_l = The fraction of those Earth-sized planets where life actually develops
• f_i = The fraction of life sites where intelligence develops
• f_c = The fraction of communicative planets (those on which electromagnetic communications technology develops)
• L = The "lifetime" of communicating civilizations

However, whilst the rationale behind the equation is sound, it is unlikely that the equation will be constrained to reasonable limits of error any time soon. The problem with the formula is that it is not used to generate or support hypotheses because it contains factors that can never be verified. The first term, R*, number of stars, is generally constrained within a few orders of magnitude. The second and third terms, f_p, stars with planets and f_e, planets with habitable conditions, are being evaluated for the star's neighborhood. Drake originally formulated the equation merely as an agenda for discussion at the Green Bank conference, but some applications of the formula had been taken literally and related to simplistic or pseudoscientific arguments. Another associated topic is the Fermi paradox, which suggests that if intelligent life is common in the universe, then there should be obvious signs of it.

Another active research area in astrobiology is planetary system formation. It has been suggested that the peculiarities of the Solar System (for example, the presence of Jupiter as a protective shield) may have greatly increased the probability of intelligent life arising on our planet.

Biology

Hydrothermal vents are able to support extremophile bacteria on Earth and may also support life in other parts of the cosmos.

Biology cannot state that a process or phenomenon, by being mathematically possible, has to exist forcibly in an extraterrestrial body. Biologists specify what is speculative and what is not. The discovery of extremophiles, organisms able to survive in extreme environments, became a core research element for astrobiologists, as they are important to understand four areas in the limits of life in planetary context: the potential for panspermia, forward contamination due to human exploration ventures, planetary colonization by humans, and the exploration of extinct and extant extraterrestrial life.

Until the 1970s, life was thought to be entirely dependent on energy from the Sun. Plants on Earth's surface capture energy from sunlight to photosynthesize sugars from carbon dioxide and water, releasing oxygen in the process that is then consumed by oxygen-respiring organisms, passing their energy up the food chain. Even life in the ocean depths, where sunlight cannot reach, was thought to obtain its nourishment either from consuming organic detritus rained down from the surface waters or from eating animals that did. The world's ability to support life was thought to depend on its access to sunlight. However, in 1977, during an exploratory dive to the Galapagos Rift in the deep-sea exploration submersible Alvin, scientists discovered colonies of giant tube worms, clams, crustaceans, mussels, and other assorted creatures clustered around undersea volcanic features known as black smokers. These creatures thrive despite having no access to sunlight, and it was soon discovered that they comprise an entirely independent ecosystem. Although most of these multicellular lifeforms need dissolved oxygen (produced by oxygenic photosynthesis) for their aerobic cellular respiration and thus are not completely independent from sunlight by themselves, the basis for their food chain is a form of bacterium that derives its energy from oxidization of reactive chemicals, such as hydrogen or hydrogen sulfide, that bubble up from the Earth's interior. Other lifeforms entirely decoupled from the energy from sunlight are green sulfur bacteria which are capturing geothermal light for anoxygenic photosynthesis or bacteria running chemolithoautotrophy based on the radioactive decay of uranium. This chemosynthesis revolutionized the study of biology and astrobiology by revealing that life need not be sun-dependent; it only requires water and an energy gradient in order to exist.

Biologists have found extremophiles that thrive in ice, boiling water, acid, alkali, the water core of nuclear reactors, salt crystals, toxic waste and in a range of other extreme habitats that were previously thought to be inhospitable for life. This opened up a new avenue in astrobiology by massively expanding the number of possible extraterrestrial habitats. Characterization of these organisms, their environments and their evolutionary pathways, is considered a crucial component to understanding how life might evolve elsewhere in the universe. For example, some organisms able to withstand exposure to the vacuum and radiation of outer space include the lichen fungi Rhizocarpon geographicum and Xanthoria elegans, the bacterium Bacillus safensis, Deinococcus radiodurans, Bacillus subtilis, yeast Saccharomyces cerevisiae, seeds from Arabidopsis thaliana ('mouse-ear cress'), as well as the invertebrate animal Tardigrade. While tardigrades are not considered true extremophiles, they are considered extremotolerant microorganisms that have contributed to the field of astrobiology. Their extreme radiation tolerance and presence of DNA protection proteins may provide answers as to whether life can survive away from the protection of the Earth's atmosphere.

Jupiter's moon, Europa, and Saturn's moon, Enceladus, are now considered the most likely locations for extant extraterrestrial life in the Solar System due to their subsurface water oceans where radiogenic and tidal heating enables liquid water to exist.

The origin of life, known as abiogenesis, distinct from the evolution of life, is another ongoing field of research. Oparin and Haldane postulated that the conditions on the early Earth were conducive to the formation of organic compounds from inorganic elements and thus to the formation of many of the chemicals common to all forms of life we see today. The study of this process, known as prebiotic chemistry, has made some progress, but it is still unclear whether or not life could have formed in such a manner on Earth. The alternative hypothesis of panspermia is that the first elements of life may have formed on another planet with even more favorable conditions (or even in interstellar space, asteroids, etc.) and then have been carried over to Earth—the panspermia hypothesis.

The cosmic dust permeating the universe contains complex organic compounds ("amorphous organic solids with a mixed aromatic-aliphatic structure") that could be created naturally, and rapidly, by stars. Further, a scientist suggested that these compounds may have been related to the development of life on Earth and said that, "If this is the case, life on Earth may have had an easier time getting started as these organics can serve as basic ingredients for life."

More than 20% of the carbon in the universe may be associated with polycyclic aromatic hydrocarbons (PAHs), possible starting materials for the formation of life. PAHs seem to have been formed shortly after the Big Bang, are widespread throughout the universe, and are associated with new stars and exoplanets. PAHs are subjected to interstellar medium conditions and are transformed through hydrogenation, oxygenation and hydroxylation, to more complex organics—"a step along the path toward amino acids and nucleotides, the raw materials of proteins and DNA, respectively".

In October 2020, astronomers proposed the idea of detecting life on distant planets by studying the shadows of trees at certain times of the day.

Astroecology

Astroecology concerns the interactions of life with space environments and resources, in planets, asteroids and comets. On a larger scale, astroecology concerns resources for life about stars in the galaxy through the cosmological future. Astroecology attempts to quantify future life in space, addressing this area of astrobiology.

Experimental astroecology investigates resources in planetary soils, using actual space materials in meteorites. The results suggest that Martian and carbonaceous chondrite materials can support bacteria, algae and plant (asparagus, potato) cultures, with high soil fertilities. The results support that life could have survived in early aqueous asteroids and on similar materials imported to Earth by dust, comets and meteorites, and that such asteroid materials can be used as soil for future space colonies.

On the largest scale, cosmoecology concerns life in the universe over cosmological times. The main sources of energy may be red giant stars and white and red dwarf stars, sustaining life for 10²⁰ years. Astroecologists suggest that their mathematical models may quantify the potential amounts of future life in space, allowing a comparable expansion in biodiversity, potentially leading to diverse intelligent life forms.

Astrogeology

Astrogeology is a planetary science discipline concerned with the geology of celestial bodies such as the planets and their moons, asteroids, comets, and meteorites. The information gathered by this discipline allows the measure of a planet's or a natural satellite's potential to develop and sustain life, or planetary habitability.

An additional discipline of astrogeology is geochemistry, which involves study of the chemical composition of the Earth and other planets, chemical processes and reactions that govern the composition of rocks and soils, the cycles of matter and energy and their interaction with the hydrosphere and the atmosphere of the planet. Specializations include cosmochemistry, biochemistry and organic geochemistry.

The fossil record provides the oldest known evidence for life on Earth. By examining the fossil evidence, paleontologists are able to better understand the types of organisms that arose on the early Earth. Some regions on Earth, such as the Pilbara in Western Australia and the McMurdo Dry Valleys of Antarctica, are also considered to be geological analogs to regions of Mars, and as such, might be able to provide clues on how to search for past life on Mars.

The various organic functional groups, composed of hydrogen, oxygen, nitrogen, phosphorus, sulfur, and a host of metals, such as iron, magnesium, and zinc, provide the enormous diversity of chemical reactions necessarily catalyzed by a living organism. Silicon, in contrast, interacts with only a few other atoms, and the large silicon molecules are monotonous compared with the combinatorial universe of organic macromolecules. Indeed, it seems likely that the basic building blocks of life anywhere will be similar to those on Earth, in the generality if not in the detail. Although terrestrial life and life that might arise independently of Earth are expected to use many similar, if not identical, building blocks, they also are expected to have some biochemical qualities that are unique. If life has had a comparable impact elsewhere in the Solar System, the relative abundances of chemicals key for its survival—whatever they maybe—could betray its presence. Whatever extraterrestrial life may be, its tendency to chemically alter its environment might just give it away.

Life in the Solar System

Europa, due to the ocean that exists under its icy surface, might host some form of microbial life.

People have long speculated about the possibility of life in settings other than Earth, however, speculation on the nature of life elsewhere often has paid little heed to constraints imposed by the nature of biochemistry. The likelihood that life throughout the universe is probably carbon-based is suggested by the fact that carbon is one of the most abundant of the higher elements. Only two of the natural atoms, carbon and silicon, are known to serve as the backbones of molecules sufficiently large to carry biological information. As the structural basis for life, one of carbon's important features is that unlike silicon, it can readily engage in the formation of chemical bonds with many other atoms, thereby allowing for the chemical versatility required to conduct the reactions of biological metabolism and propagation.

Discussion on where in the Solar System life might occur was limited historically by the understanding that life relies ultimately on light and warmth from the Sun and, therefore, is restricted to the surfaces of planets. The four most likely candidates for life in the Solar System are the planet Mars, the Jovian moon Europa, and Saturn's moons Titan, and Enceladus.

Mars, Enceladus and Europa are considered likely candidates in the search for life primarily because they may have underground liquid water, a molecule essential for life as we know it for its use as a solvent in cells. Water on Mars is found frozen in its polar ice caps, and newly carved gullies recently observed on Mars suggest that liquid water may exist, at least transiently, on the planet's surface. At the Martian low temperatures and low pressure, liquid water is likely to be highly saline. As for Europa and Enceladus, large global oceans of liquid water exist beneath these moons' icy outer crusts. This water may be warmed to a liquid state by volcanic vents on the ocean floor, but the primary source of heat is probably tidal heating. On 11 December 2013, NASA reported the detection of "clay-like minerals" (specifically, phyllosilicates), often associated with organic materials, on the icy crust of Europa. The presence of the minerals may have been the result of a collision with an asteroid or comet according to the scientists. Additionally, on 27 June 2018, astronomers reported the detection of complex macromolecular organics on Enceladus and, according to NASA scientists in May 2011, "is emerging as the most habitable spot beyond Earth in the Solar System for life as we know it".

Another planetary body that could potentially sustain extraterrestrial life is Saturn's largest moon, Titan. Titan has been described as having conditions similar to those of early Earth. On its surface, scientists have discovered the first liquid lakes outside Earth, but these lakes seem to be composed of ethane and/or methane, not water. Some scientists think it possible that these liquid hydrocarbons might take the place of water in living cells different from those on Earth. After Cassini data were studied, it was reported in March 2008 that Titan may also have an underground ocean composed of liquid water and ammonia.

Phosphine has been detected in the atmosphere of the planet Venus. There are no known abiotic processes on the planet that could cause its presence. Given that Venus has the hottest surface temperature of any planet in the solar system, Venusian life, if it exists, is most likely limited to extremophile microorganisms that float in the planet's upper atmosphere, where conditions are almost Earth-like.

Measuring the ratio of hydrogen and methane levels on Mars may help determine the likelihood of life on Mars. According to the scientists, "...low H₂/CH₄ ratios (less than approximately 40) indicate that life is likely present and active." Other scientists have recently reported methods of detecting hydrogen and methane in extraterrestrial atmospheres.

Complex organic compounds of life, including uracil, cytosine and thymine, have been formed in a laboratory under outer space conditions, using starting chemicals such as pyrimidine, found in meteorites. Pyrimidine, like polycyclic aromatic hydrocarbons (PAHs), is the most carbon-rich chemical found in the universe.

Rare Earth hypothesis

The Rare Earth hypothesis postulates that multicellular life forms found on Earth may actually be more of a rarity than scientists assume. It provides a possible answer to the Fermi paradox which suggests, "If extraterrestrial aliens are common, why aren't they obvious?" It is apparently in opposition to the principle of mediocrity, assumed by famed astronomers Frank Drake, Carl Sagan, and others. The Principle of Mediocrity suggests that life on Earth is not exceptional, and it is more than likely to be found on innumerable other worlds.

Research

The systematic search for possible life outside Earth is a valid multidisciplinary scientific endeavor. However, hypotheses and predictions as to its existence and origin vary widely, and at the present, the development of hypotheses firmly grounded on science may be considered astrobiology's most concrete practical application. It has been proposed that viruses are likely to be encountered on other life-bearing planets, and may be present even if there are no biological cells.

Research outcomes

What biosignatures does life produce?

As of 2019, no evidence of extraterrestrial life has been identified. Examination of the Allan Hills 84001 meteorite, which was recovered in Antarctica in 1984 and originated from Mars, is thought by David McKay, as well as few other scientists, to contain microfossils of extraterrestrial origin; this interpretation is controversial.

Asteroid(s) may have transported life to Earth.

Yamato 000593, the second largest meteorite from Mars, was found on Earth in 2000. At a microscopic level, spheres are found in the meteorite that are rich in carbon compared to surrounding areas that lack such spheres. The carbon-rich spheres may have been formed by biotic activity according to some NASA scientists.

On 5 March 2011, Richard B. Hoover, a scientist with the Marshall Space Flight Center, speculated on the finding of alleged microfossils similar to cyanobacteria in CI1 carbonaceous meteorites in the fringe Journal of Cosmology, a story widely reported on by mainstream media. However, NASA formally distanced itself from Hoover's claim. According to American astrophysicist Neil deGrasse Tyson: "At the moment, life on Earth is the only known life in the universe, but there are compelling arguments to suggest we are not alone."

Extreme environments on Earth

On 17 March 2013, researchers reported that microbial life forms thrive in the Mariana Trench, the deepest spot on the Earth. Other researchers reported that microbes thrive inside rocks up to 1,900 feet (580 m) below the sea floor under 8,500 feet (2,600 m) of ocean off the coast of the northwestern United States. According to one of the researchers, "You can find microbes everywhere—they're extremely adaptable to conditions, and survive wherever they are." Evidence of perchlorates have been found throughout the solar system, and specifically on Mars. Dr. Kennda Lynch discovered the first known instance of perchlorates and perchlorates-reducing microbes in a paleolake in Pilot Valley, Utah. These finds expand the potential habitability of certain niches of other planets.

Methane

In 2004, the spectral signature of methane (CH
₄) was detected in the Martian atmosphere by both Earth-based telescopes as well as by the Mars Express orbiter. Because of solar radiation and cosmic radiation, methane is predicted to disappear from the Martian atmosphere within several years, so the gas must be actively replenished in order to maintain the present concentration. On 7 June 2018, NASA announced a cyclical seasonal variation in atmospheric methane, which may be produced by geological or biological sources. The European ExoMars Trace Gas Orbiter is currently measuring and mapping the atmospheric methane.

Planetary systems

It is possible that some exoplanets may have moons with solid surfaces or liquid oceans that are hospitable. Most of the planets so far discovered outside the Solar System are hot gas giants thought to be inhospitable to life, so it is not yet known whether the Solar System, with a warm, rocky, metal-rich inner planet such as Earth, is of an aberrant composition. Improved detection methods and increased observation time will undoubtedly discover more planetary systems, and possibly some more like ours. For example, NASA's Kepler Mission seeks to discover Earth-sized planets around other stars by measuring minute changes in the star's light curve as the planet passes between the star and the spacecraft. Progress in infrared astronomy and submillimeter astronomy has revealed the constituents of other star systems.

Planetary habitability

Efforts to answer questions such as the abundance of potentially habitable planets in habitable zones and chemical precursors have had much success. Numerous extrasolar planets have been detected using the wobble method and transit method, showing that planets around other stars are more numerous than previously postulated. The first Earth-sized extrasolar planet to be discovered within its star's habitable zone is Gliese 581 c.

Extremophiles

Studying extremophiles is useful for understanding the possible origin of life on Earth as well as for finding the most likely candidates for future colonization of other planets. The aim is to detect those organisms that are able to survive space travel conditions and to maintain the proliferating capacity. The best candidates are extremophiles, since they have adapted to survive in different kind of extreme conditions on earth. During the course of evolution, extremophiles have developed various strategies to survive the different stress conditions of different extreme environments. These stress responses could also allow them to survive in harsh space conditions.

Thermophilic species G. thermantarcticus is a good example of a microorganism that could survive space travel. It is a bacterium of the spore-forming genus Bacillus. The formation of spores allows for it to survive extreme environments while still being able to restart cellular growth. It is capable of effectively protecting its DNA, membrane and proteins integrity in different extreme conditions (desiccation, temperatures up to -196 °C, UVC and C-ray radiation...). It is also able to repair the damage produced by space environment.

By understanding how extremophilic organisms can survive the Earth's extreme environments, we can also understand how microorganisms could have survived space travel and how the panspermia hypothesis could be possible.

Missions

Research into the environmental limits of life and the workings of extreme ecosystems is ongoing, enabling researchers to better predict what planetary environments might be most likely to harbor life. Missions such as the Phoenix lander, Mars Science Laboratory, ExoMars, Mars 2020 rover to Mars, and the Cassini probe to Saturn's moons aim to further explore the possibilities of life on other planets in the Solar System.

Viking program

The two Viking landers each carried four types of biological experiments to the surface of Mars in the late 1970s. These were the only Mars landers to carry out experiments looking specifically for metabolism by current microbial life on Mars. The landers used a robotic arm to collect soil samples into sealed test containers on the craft. The two landers were identical, so the same tests were carried out at two places on Mars' surface; Viking 1 near the equator and Viking 2 further north. The result was inconclusive, and is still disputed by some scientists.

Norman Horowitz was the chief of the Jet Propulsion Laboratory bioscience section for the Mariner and Viking missions from 1965 to 1976. Horowitz considered that the great versatility of the carbon atom makes it the element most likely to provide solutions, even exotic solutions, to the problems of survival of life on other planets. However, he also considered that the conditions found on Mars were incompatible with carbon based life.

Beagle 2

Replica of the 33.2 kg Beagle-2 lander

 

Mars Science Laboratory rover concept artwork

Beagle 2 was an unsuccessful British Mars lander that formed part of the European Space Agency's 2003 Mars Express mission. Its primary purpose was to search for signs of life on Mars, past or present. Although it landed safely, it was unable to correctly deploy its solar panels and telecom antenna.

EXPOSE

EXPOSE is a multi-user facility mounted in 2008 outside the International Space Station dedicated to astrobiology. EXPOSE was developed by the European Space Agency (ESA) for long-term spaceflights that allow exposure of organic chemicals and biological samples to outer space in low Earth orbit.

Mars Science Laboratory

The Mars Science Laboratory (MSL) mission landed the Curiosity rover that is currently in operation on Mars. It was launched 26 November 2011, and landed at Gale Crater on 6 August 2012. Mission objectives are to help assess Mars' habitability and in doing so, determine whether Mars is or has ever been able to support life, collect data for a future human mission, study Martian geology, its climate, and further assess the role that water, an essential ingredient for life as we know it, played in forming minerals on Mars.

Tanpopo

The Tanpopo mission is an orbital astrobiology experiment investigating the potential interplanetary transfer of life, organic compounds, and possible terrestrial particles in the low Earth orbit. The purpose is to assess the panspermia hypothesis and the possibility of natural interplanetary transport of microbial life as well as prebiotic organic compounds. Early mission results show evidence that some clumps of microorganism can survive for at least one year in space. This may support the idea that clumps greater than 0.5 millimeters of microorganisms could be one way for life to spread from planet to planet.

ExoMars rover

ExoMars rover model

ExoMars is a robotic mission to Mars to search for possible biosignatures of Martian life, past or present. This astrobiological mission is currently under development by the European Space Agency (ESA) in partnership with the Russian Federal Space Agency (Roscosmos); it is planned for a 2022 launch.

Mars 2020

Artist's rendition of the Perseverance rover on Mars, with the mini-helicopter Ingenuity in front

Mars 2020 successfully landed its rover Perseverance in Jezero Crater on 18 February 2021. It will investigate environments on Mars relevant to astrobiology, investigate its surface geological processes and history, including the assessment of its past habitability and potential for preservation of biosignatures and biomolecules within accessible geological materials. The Science Definition Team is proposing the rover collect and package at least 31 samples of rock cores and soil for a later mission to bring back for more definitive analysis in laboratories on Earth. The rover could make measurements and technology demonstrations to help designers of a human expedition understand any hazards posed by Martian dust and demonstrate how to collect carbon dioxide (CO₂), which could be a resource for making molecular oxygen (O₂) and rocket fuel.

Europa Clipper

Europa Clipper is a mission planned by NASA for a 2025 launch that will conduct detailed reconnaissance of Jupiter's moon Europa and will investigate whether its internal ocean could harbor conditions suitable for life. It will also aid in the selection of future landing sites.

Proposed concepts

Icebreaker Life

Icebreaker Life is a lander mission that proposed for NASA's Discovery Program for the 2021 launch opportunity, but it was not selected for development. It would have had a stationary lander that would be a near copy of the successful 2008 Phoenix and it would have carried an upgraded astrobiology scientific payload, including a 1-meter-long core drill to sample ice-cemented ground in the northern plains to conduct a search for organic molecules and evidence of current or past life on Mars. One of the key goals of the Icebreaker Life mission is to test the hypothesis that the ice-rich ground in the polar regions has significant concentrations of organics due to protection by the ice from oxidants and radiation.

Journey to Enceladus and Titan

Journey to Enceladus and Titan (JET) is an astrobiology mission concept to assess the habitability potential of Saturn's moons Enceladus and Titan by means of an orbiter.

Enceladus Life Finder

Enceladus Life Finder (ELF) is a proposed astrobiology mission concept for a space probe intended to assess the habitability of the internal aquatic ocean of Enceladus, Saturn's sixth-largest moon.

Life Investigation For Enceladus

Life Investigation For Enceladus (LIFE) is a proposed astrobiology sample-return mission concept. The spacecraft would enter into Saturn orbit and enable multiple flybys through Enceladus' icy plumes to collect icy plume particles and volatiles and return them to Earth on a capsule. The spacecraft may sample Enceladus' plumes, the E ring of Saturn, and the upper atmosphere of Titan.

Oceanus is an orbiter proposed in 2017 for the New Frontiers mission No. 4. It would travel to the moon of Saturn, Titan, to assess its habitability. Oceanus' objectives are to reveal Titan's organic chemistry, geology, gravity, topography, collect 3D reconnaissance data, catalog the organics and determine where they may interact with liquid water.

Explorer of Enceladus and Titan

Explorer of Enceladus and Titan (E²T) is an orbiter mission concept that would investigate the evolution and habitability of the Saturnian satellites Enceladus and Titan. The mission concept was proposed in 2017 by the European Space Agency.