Genetically modified plants have been engineered for
scientific research, to create new colours in plants, deliver vaccines,
and to create enhanced crops. Many plant cells are pluripotent,
meaning that a single cell from a mature plant can be harvested and
then under the right conditions form a new plant. This ability can be
taken advantage of by genetic engineers; by selecting for cells that
have been successfully transformed in an adult plant a new plant can
then be grown that contains the transgene in every cell through a
process known as tissue culture.
Research
Much of the advances in the field genetic engineering has come from experimentation with tobacco. Major advances in tissue culture and plant cellular mechanisms for a wide range of plants has originated from systems developed in tobacco.
It was the first plant to be genetically engineered and is considered a
model organism for not only genetic engineering, but a range of other
fields. As such the transgenic tools and procedures are well established making it one of the easiest plants to transform. Another major model organism relevant to genetic engineering is Arabidopsis thaliana. Its small genome and short life cycle makes it easy to manipulate and it contains many homolgs to important crop species. It was the first plant sequenced, has abundant bioinformatic resources and can be transformed by simply dipping a flower in a transformed Agrobacterium solution.
In research, plants are engineered to help discover the functions
of certain genes. The simplest way to do this is to remove the gene and
see what phenotype develops compared to the wild type form. Any differences are possibly the result of the missing gene. Unlike mutagenisis, genetic engineering allows targeted removal without disrupting other genes in the organism. Some genes are only expressed in certain tissue, so reporter genes, like GUS, can be attached to the gene of interest allowing visualisation of the location.
Other ways to test a gene is to alter it slightly and then return it to
the plant and see if it still has the same effect on phenotype. Other
strategies include attaching the gene to a strong promoter and see what happens when it is over expressed, forcing a gene to be expressed in a different location or at different developmental stages.
Ornamental
Suntory "blue" rose
Kenyans examining insect-resistant transgenic Bt corn
Some genetically modified plants are purely ornamental. They are modified for lower color, fragrance, flower shape and plant architecture. The first genetically modified ornamentals commercialised altered colour. Carnations were released in 1997, with the most popular genetically modified organism, a blue rose (actually lavender or mauve) created in 2004. The roses are sold in Japan, the United States, and Canada. Other genetically modified ornamentals include Chrysanthemum and Petunia.
As well as increasing aesthetic value there are plans to develop
ornamentals that use less water or are resistant to the cold, which
would allow them to be grown outside their natural environments.
Conservation
It
has been proposed to genetically modify some plant species threatened
by extinction to be resistant invasive plants and diseases, such as the emerald ash borer in North American and the fungal disease, Ceratocystis platani, in European plane trees. The papaya ringspot virus (PRSV) devastated papaya trees in Hawaii in the twentieth century until transgenic papaya plants were given pathogen-derived resistance.
However, genetic modification for conservation in plants remains mainly
speculative. A unique concern is that a transgenic species may no
longer bear enough resemblance to the original species to truly claim
that the original species is being conserved. Instead, the transgenic
species may be genetically different enough to be considered a new
species, thus diminishing the conservation worth of genetic
modification.
Crops
Genetically modified crops are genetically modified plants that are used in agriculture.
The first crops provided are used for animal or human food and provide
resistance to certain pests, diseases, environmental conditions,
spoilage or chemical treatments (e.g. resistance to a herbicide). The second generation of crops aimed to improve the quality, often by altering the nutrient profile. Third generation genetically modified crops can be used for non-food purposes, including the production of pharmaceutical agents, biofuels, and other industrially useful goods, as well as for bioremediation.
There are three main aims to agricultural advancement; increased production, improved conditions for agricultural workers and sustainability.
GM crops contribute by improving harvests through reducing insect
pressure, increasing nutrient value and tolerating different abiotic stresses. Despite this potential, as of 2018, the commercialised crops are limited mostly to cash crops
like cotton, soybean, maize and canola and the vast majority of the
introduced traits provide either herbicide tolerance or insect
resistance. Soybeans accounted for half of all genetically modified crops planted in 2014.
Adoption by farmers has been rapid, between 1996 and 2013, the total
surface area of land cultivated with GM crops increased by a factor of
100, from 17,000 square kilometers (4,200,000 acres) to 1,750,000 km2 (432 million acres). Geographically though the spread has been very uneven, with strong growth in the Americas and parts of Asia and little in Europe and Africa. Its socioeconomic spread has been more even, with approximately 54% of worldwide GM crops grown in developing countries in 2013.
Food
The majority of GM crops have been modified to be resistant to selected herbicides, usually a glyphosate or glufosinate
based one. Genetically modified crops engineered to resist herbicides
are now more available than conventionally bred resistant varieties; in the USA 93% of soybeans and most of the GM maize grown is glyphosate tolerant. Most currently available genes used to engineer insect resistance come from the Bacillus thuringiensis bacterium. Most are in the form of delta endotoxin genes known as cry proteins, while a few use the genes that encode for vegetative insecticidal proteins. The only gene commercially used to provide insect protection that does not originate from B. thuringiensis is the Cowpea trypsin inhibitor (CpTI). CpTI was first approved for use cotton in 1999 and is currently undergoing trials in rice.
Less than one percent of GM crops contained other traits, which include
providing virus resistance, delaying senescence, modifying flower
colour and altering the plants composition.
Golden rice is the most well known GM crop that is aimed at increasing nutrient value. It has been engineered with three genes that biosynthesise beta-carotene, a precursor of vitamin A, in the edible parts of rice. It is intended to produce a fortified food to be grown and consumed in areas with a shortage of dietary vitamin A. a deficiency which each year is estimated to kill 670,000 children under the age of 5 and cause an additional 500,000 cases of irreversible childhood blindness. The original golden rice produced 1.6μg/g of the carotenoids, with further development increasing this 23 times. In 2018 it gained its first approvals for use as food.
Biopharmaceuticals
Plants and plant cells have been genetically engineered for production of biopharmaceuticals in bioreactors, a process known as Pharming. Work has been done with duckweed Lemna minor, the algae Chlamydomonas reinhardtii and the moss Physcomitrella patens. Biopharmaceuticals produced include cytokines, hormones, antibodies, enzymes
and vaccines, most of which are accumulated in the plant seeds. Many
drugs also contain natural plant ingredients and the pathways that lead
to their production have been genetically altered or transferred to
other plant species to produce greater volume and better products. Other options for bioreactors are biopolymers and biofuels. Unlike bacteria, plants can modify the proteins post-translationally, allowing them to make more complex molecules.They also pose less risk of being contaminated. Therapeutics have been cultured in transgenic carrot and tobacco cells, including a drug treatment for Gaucher's disease.
Vaccines
Vaccine
production and storage has great potential in transgenic plants.
Vaccines are expensive to produce, transport and administer, so having a
system that could produce them locally would allow greater access to
poorer and developing areas.
As well as purifying vaccines expressed in plants it is also possible
to produce edible vaccines in plants. Edible vaccines stimulate the immune system
when ingested to protect against certain diseases. Being stored in
plants reduces the long-term cost as they can be disseminated without
the need for cold storage, don't need to be purified and have long term
stability. Also being housed within plant cells provides some protection
from the gut acids upon digestion. However the cost of developing,
regulating and containing transgenic plants is high, leading to most
current plant-based vaccine development being applied to veterinary medicine, where the controls are not as strict.
