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Wednesday, January 22, 2025

Ocean world

From Wikipedia, the free encyclopedia
https://en.wikipedia.org/wiki/Ocean_world
Earth's surface is dominated by the ocean, which forms 75% of Earth's surface.

An ocean world, ocean planet or water world is a type of planet or natural satellite that contains a substantial amount of water in the form of oceans, as part of its hydrosphere, either beneath the surface, as subsurface oceans, or on the surface, potentially submerging all dry land. The term ocean world is also used sometimes for astronomical bodies with an ocean composed of a different fluid or thalassogen, such as lava (the case of Io), ammonia (in a eutectic mixture with water, as is likely the case of Titan's inner ocean) or hydrocarbons (like on Titan's surface, which could be the most abundant kind of exosea).[] The study of extraterrestrial oceans is referred to as planetary oceanography.

Earth is the only astronomical object known to presently have bodies of liquid water on its surface, although subsurface oceans are suspected to exist on Jupiter's moons Europa and Ganymede and Saturn's moons Enceladus and Titan.  Several exoplanets have been found with the right conditions to support liquid water. There are also considerable amounts of subsurface water found on Earth, mostly in the form of aquifers.[9] For exoplanets, current technology cannot directly observe liquid surface water, so atmospheric water vapor may be used as a proxy. The characteristics of ocean worlds provide clues to their history and the formation and evolution of the Solar System as a whole. Of additional interest is their potential to originate and host life.

In June 2020, NASA scientists reported that it is likely that exoplanets with oceans are common in the Milky Way galaxy, based on mathematical modeling studies.

Overview

Solar System planetary bodies

Diagram of the interior of Enceladus

Ocean worlds are of interest to astrobiologists for their potential to develop life and sustain biological activity over geological timescales. Major moons and dwarf planets in the Solar System thought to harbor subsurface oceans are of interest because they can be reached and studied by space probes, in contrast to exoplanets, which are light-years away, beyond the reach of current technology. The best-established water worlds in the Solar System, other than the Earth, are Callisto, Enceladus, Europa, Ganymede, and Titan. Europa and Enceladus are considered compelling targets for exploration due to their thin outer crusts and cryovolcanic features.

Other bodies in the Solar System are considered candidates to host subsurface oceans based upon a single type of observation or by theoretical modeling, including Ariel, Titania, Umbriel, Ceres, Dione, Mimas, Miranda, Oberon, Pluto, Triton, Eris, and Makemake.

Exoplanets

A set of exoplanets of varying size containing water, compared with the Earth (artist concept; 17 August 2018)
Exoplanet population with purely oceanic worlds as transition group with ice giants between gas giants and lava or rocky planets

Outside the Solar System, exoplanets that have been described as candidate ocean worlds include GJ 1214 b, Kepler-22b, Kepler-62e, Kepler-62f, and the planets of Kepler-11 and TRAPPIST-1.

More recently, the exoplanets TOI-1452 b, Kepler-138c, and Kepler-138d have been found to have densities consistent with large fractions of their mass being composed of water. Additionally, models of the massive rocky planet LHS 1140 b suggest its surface may be covered in a deep ocean.

Although 70.8% of all Earth's surface is covered in water, water accounts for only 0.05% of Earth's mass. An extraterrestrial ocean could be so deep and dense that even at high temperatures the pressure would turn the water into ice. The immense pressures of many thousands of bar in the lower regions of such oceans, could lead to the formation of a mantle of exotic forms of ice such as ice V. This ice would not necessarily be as cold as conventional ice. If the planet is close enough to its star that the water reaches its boiling point, the water will become supercritical and lack a well-defined surface. Even on cooler water-dominated planets, the atmosphere can be much thicker than that of Earth, and composed largely of water vapor, producing a very strong greenhouse effect. Such planets would have to be small enough not to be able to retain a thick envelope of hydrogen and helium, or be close enough to their primary star to be stripped of these light elements. Otherwise, they would form a warmer version of an ice giant instead, like Uranus and Neptune.

History

Important preliminary theoretical work was carried out prior to the planetary missions of the 1970s. In particular, Lewis showed in 1971 that radioactive decay alone was likely sufficient to produce subsurface oceans in large moons, especially if ammonia (NH
3
) were present. Peale and Cassen figured out in 1979 the important role of tidal heating (aka: tidal flexing) on satellite evolution and structure. The first confirmed detection of an exoplanet was in 1992. Marc Kuchner in 2003 and Alain Léger et al figured in 2004 that a small number of icy planets that form in the region beyond the snow line can migrate inward to ~1 AU, where the outer layers subsequently melt.

The cumulative evidence collected by the Hubble Space Telescope, as well as Pioneer, Galileo, Voyager, Cassini–Huygens, and New Horizons missions, strongly indicate that several outer Solar System bodies harbour internal liquid water oceans under an insulating ice shell. Meanwhile, the Kepler space observatory, launched on March 7, 2009, has discovered thousands of exoplanets, about 50 of them of Earth-size in or near habitable zones.

Planets of many masses, sizes, and orbits have been detected, illustrating not only the variable nature of planet formation but also a subsequent migration through the circumstellar disc from the planet's place of origin. As of 24 July 2024, there are 7,026 confirmed exoplanets in 4,949 planetary systems, with 1007 systems having more than one planet.

In June 2020, NASA scientists reported that it is likely that exoplanets with oceans may be common in the Milky Way galaxy, based on mathematical modeling studies.

In August 2022, TOI-1452 b, a super-Earth exoplanet with potential deep oceans that is 99 light-years from Earth, was discovered by the Transiting Exoplanet Survey Satellite.

Formation

Atacama Large Millimeter Array image of HL Tauri, a protoplanetary disk

Planetary objects that form in the outer Solar System begin as a comet-like mixture of roughly half water and half rock by mass, displaying a density lower than that of rocky planets. Icy planets and moons that form near the frost line should contain mostly H
2
O
and silicates. Those that form farther out can acquire ammonia (NH
3
) and methane (CH
4
) as hydrates, together with CO, N
2
, and CO
2
.

Planets that form prior to the dissipation of the gaseous circumstellar disk experience strong torques that can induce rapid inward migration into the habitable zone, especially for planets in the terrestrial mass range. Since water is highly soluble in magma, a large fraction of the planet's water content will initially be trapped in the mantle. As the planet cools and the mantle begins to solidify from the bottom up, large amounts of water (between 60% and 99% of the total amount in the mantle) are exsolved to form a steam atmosphere, which may eventually condense to form an ocean. Ocean formation requires differentiation, and a heat source, either radioactive decay, tidal heating, or the early luminosity of the parent body. Unfortunately, the initial conditions following accretion are theoretically incomplete.

Planets that formed in the outer, water-rich regions of a disk and migrated inward are more likely to have abundant water. Conversely, planets that formed close to their host stars are less likely to have water because the primordial disks of gas and dust are thought to have hot and dry inner regions. So if a water world is found close to a star, it would be strong evidence for migration and ex situ formation, because insufficient volatiles exist near the star for in situ formation. Simulations of Solar System formation and of extra-solar system formation have shown that planets are likely to migrate inward (i.e., toward the star) as they form. Outward migration may also occur under particular conditions. Inward migration presents the possibility that icy planets could move to orbits where their ice melts into liquid form, turning them into ocean planets. This possibility was first discussed in the astronomical literature by Marc Kuchner in 2003.

Structure

The internal structure of an icy astronomical body is generally deduced from measurements of its bulk density, gravity moments, and shape. Determining the moment of inertia of a body can help assess whether it has undergone differentiation (separation into rock-ice layers) or not. Shape or gravity measurements can in some cases be used to infer the moment of inertia – if the body is in hydrostatic equilibrium (i.e. behaving like a fluid on long timescales). Proving that a body is in hydrostatic equilibrium is extremely difficult, but by using a combination of shape and gravity data, the hydrostatic contributions can be deduced.ecific techniques to detect inner oceans include magnetic induction, geodesy, librations, axial tilt, tidal response, radar sounding, compositional evidence, and surface features.

Artist's cut-away representation of the internal structure of Ganymede, with a liquid water ocean "sandwiched" between two ice layers. Layers drawn to scale.

A generic icy moon will consist of a water layer sitting atop a silicate core. For a small satellite like Enceladus, an ocean will sit directly above the silicates and below a solid icy shell, but for a larger ice-rich body like Ganymede, pressures are sufficiently high that the ice at depth will transform to higher pressure phases, effectively forming a "water sandwich" with an ocean located between ice shells. An important difference between these two cases is that for the small satellite the ocean is in direct contact with the silicates, which may provide hydrothermal and chemical energy and nutrients to simple life forms. Because of the varying pressure at depth, models of a water world may include "steam, liquid, superfluid, high-pressure ices, and plasma phases" of water. Some of the solid-phase water could be in the form of ice VII.

Maintaining a subsurface ocean depends on the rate of internal heating compared with the rate at which heat is removed, and the freezing point of the liquid. Ocean survival and tidal heating are thus intimately linked.

Smaller ocean planets would have less dense atmospheres and lower gravity; thus, liquid could evaporate much more easily than on more massive ocean planets. Simulations suggest that planets and satellites of less than one Earth mass could have liquid oceans driven by hydrothermal activity, radiogenic heating, or tidal flexing. Where fluid-rock interactions propagate slowly into a deep brittle layer, thermal energy from serpentinization may be the primary cause of hydrothermal activity in small ocean planets. The dynamics of global oceans beneath tidally flexing ice shells represents a significant set of challenges which have barely begun to be explored. The extent to which cryovolcanism occurs is a subject of some debate, as water, being denser than ice by about 8%, has difficulty erupting under normal circumstances. Nevertheless, imaging data from the Voyager 2, Cassini-Huygens, Galileo and New Horizons spacecraft revealed cryovolcanic surface features on several of the icy bodies in our own solar system. Recent studies suggest that cryovolcanism may occur on ocean planets that harbor internal oceans beneath layers of surface ice as it does on the icy moons Enceladus and Europa in our own solar system.

Liquid water oceans on extrasolar planets could be significantly deeper than the Earth’s ocean, which has an average depth of 3.7 km. Depending on the planet’s gravity and surface conditions, exoplanet oceans could be up to hundreds of times deeper. For example, a planet with a 300 K surface can possess liquid water oceans with depths from 30–500 km, depending on its mass and composition.

Atmospheric models

Artist depiction of a hycean planet, a large ocean world with a hydrogen atmosphere

To allow surface water to be liquid for long periods of time, a planet—or moon—must orbit within the habitable zone (HZ), possess a protective magnetic field, and have the gravitational pull needed to retain an ample amount of atmospheric pressure. If the planet's gravity cannot sustain that, then all the water will eventually evaporate into outer space. A strong planetary magnetosphere, maintained by internal dynamo action in an electrically conducting fluid layer, is helpful for shielding the upper atmosphere from stellar wind mass loss and retaining water over long geological time scales.

A planet's atmosphere forms from outgassing during planet formation or is gravitationally captured from the surrounding protoplanetary nebula. The surface temperature on an exoplanet is governed by the atmosphere's greenhouse gases (or lack thereof), so an atmosphere can be detectable in the form of upwelling infrared radiation because the greenhouse gases absorb and re-radiate energy from the host star. Ice-rich planets that have migrated inward into orbit too close to their host stars may develop thick steamy atmospheres but still retain their volatiles for billions of years, even if their atmospheres undergo slow hydrodynamic escape. Ultraviolet photons are not only biologically harmful but can drive fast atmospheric escape that leads to the erosion of planetary atmospheres; photolysis of water vapor, and hydrogen/oxygen escape to space can lead to the loss of several Earth oceans of water from planets throughout the habitable zone, regardless of whether the escape is energy-limited or diffusion-limited. The amount of water lost seems proportional with the planet mass, since the diffusion-limited hydrogen escape flux is proportional to the planet surface gravity.

During a runaway greenhouse effect, water vapor reaches the stratosphere, where it is easily broken down (photolyzed) by ultraviolet radiation (UV). Heating of the upper atmosphere by UV radiation can then drive a hydrodynamic wind that carries the hydrogen (and potentially some of the oxygen) to space, leading to the irreversible loss of a planet's surface water, oxidation of the surface, and possible accumulation of oxygen in the atmosphere. The fate of a given planet's atmosphere strongly depends on the extreme ultraviolet flux, the duration of the runaway regime, the initial water content, and the rate at which oxygen is absorbed by the surface. Volatile-rich planets should be more common in the habitable zones of young stars and M-type stars.

Scientists have proposed Hycean planets, ocean planets with a thick atmosphere made mainly of hydrogen. Those planets would have a wide range area around their star where they could orbit and have liquid water. However, those models worked on rather simplistic approaches to the planetary atmosphere. More complex studies showed that hydrogen reacts differently to starlight's wavelengths than heavier elements like nitrogen and oxygen. If such a planet, with an atmospheric pressure 10 to 20 heavier than Earth's, was located at 1 astronomical unit (AU) from their star their water bodies would boil. Those studies now place the habitable zone of such worlds at 3.85 AU, and 1.6 AU if it had a similar atmospheric pressure to Earth.

Composition models

There are challenges in examining an exoplanetary surface and its atmosphere, as cloud coverage influences the atmospheric temperature, structure as well as the observability of spectral features. However, planets composed of large quantities of water that reside in the habitable zone (HZ) are expected to have distinct geophysics and geochemistry of their surface and atmosphere. For example, in the case of exoplanets Kepler-62e and -62f, they could possess a liquid ocean outer surface, a steam atmosphere, or a full cover of surface Ice I, depending on their orbit within the HZ and the magnitude of their greenhouse effect. Several other surface and interior processes affect the atmospheric composition, including but not limited to the ocean fraction for dissolution of CO
2
and for atmospheric relative humidity, redox state of the planetary surface and interior, acidity levels of the oceans, planetary albedo, and surface gravity.

The atmospheric structure, as well as the resulting HZ limits, depend on the density of a planet's atmosphere, shifting the HZ outward for lower mass and inward for higher mass planets. Theory, as well as computer models suggest that atmospheric composition for water planets in the habitable zone (HZ) should not differ substantially from those of land-ocean planets. For modeling purposes, it is assumed that the initial composition of icy planetesimals that assemble into water planets is similar to that of comets: mostly water (H
2
O
), and some ammonia (NH
3
), and carbon dioxide (CO
2
). An initial composition of ice similar to that of comets leads to an atmospheric model composition of 90% H
2
O
, 5% NH
3
, and 5% CO
2
.

Atmospheric models for Kepler-62f show that an atmospheric pressure of between 1.6 bar and 5 bar of CO
2
are needed to warm the surface temperature above freezing, leading to a scaled surface pressure of 0.56–1.32 times Earth's.

Oceanography

It is suggested that strong ocean currents exist in Enceladus, Titan, Ganymede, and Europa. In Enceladus, oceanic heat flux inferred from ice shell thickness suggests the upwelling of warm water at the poles and downwelling of colder water at low latitudes.  Europa is predicted to have an equatorial upwelling of warm water with greater heat transfer at low latitudes.  Global scale currents are organized into three zonal and two equatorial circulation cells, convecting internal heat toward the surface, especially in equatorial regions.  Titan and Ganymede are hypothesized to behave as a non-rotating system and have no coherent heat transfer patterns.

Definitions

According to Lunine, "oceans" have been defined as "stable, globe-girdling bodies of liquid water." In addition, "Ocean worlds is the label given to objects in the solar system that host stable, globe-girdling bodies of liquid water," in contrast to the terms "'ocean planet' and 'water world', both of which refer to exoplanets (planets orbiting other stars) with substantial mass fractions of water in their bulk compositions."

Astrobiology

The characteristics of ocean worlds or ocean planets provide clues to their history, and the formation and evolution of the Solar System as a whole. Of additional interest is their potential to form and host life. Life as we know it requires liquid water, a source of energy, and nutrients, and all three key requirements can potentially be satisfied within some of these bodies, that may offer the possibility for sustaining simple biological activity over geological timescales. In August 2018, researchers reported that water worlds could support life.

An ocean world's habitation by Earth-like life is limited if the planet is completely covered by liquid water at the surface, even more restricted if a pressurized, solid ice layer is located between the global ocean and the lower rocky mantle. Simulations of a hypothetical ocean world covered by five Earth oceans' worth of water indicate the water would not contain enough phosphorus and other nutrients for Earth-like oxygen-producing ocean organisms such as plankton to evolve. On Earth, phosphorus is washed into the oceans by rainwater hitting rocks on exposed land, so the mechanism would not work on an ocean world. Simulations of ocean planets with 50 Earth oceans' worth of water indicate the pressure on the sea floor would be so immense that the planet's interior would not sustain plate tectonics to cause volcanism to provide the right chemical environment for terrestrial life.

On the other hand, small bodies such as Europa and Enceladus are regarded as particularly habitable environments because the theorized locations of their oceans would almost certainly leave them in direct contact with the underlying silicate core, a potential source of both heat and biologically important chemical elements. The surface geological activity of these bodies may also lead to the transport to the oceans of biologically-important building blocks implanted at the surface, such as organic molecules from comets or tholins, formed by solar ultraviolet irradiation of simple organic compounds such as methane or ethane, often in combination with nitrogen.

Oxygen

Molecular oxygen (O
2
) can be produced by geophysical processes, as well as a byproduct of photosynthesis by life forms, so although encouraging, O
2
is not a reliable biosignature. In fact, planets with high concentration of O
2
in their atmosphere may be uninhabitable. Abiogenesis in the presence of massive amounts of atmospheric oxygen could be difficult because early organisms relied on the free energy available in redox reactions involving a variety of hydrogen compounds; on an O
2
-rich planet, organisms would have to compete with the oxygen for this free energy.

Supercritical fluid

From Wikipedia, the free encyclopedia

A supercritical fluid (SCF) is a substance at a temperature and pressure above its critical point, where distinct liquid and gas phases do not exist, but below the pressure required to compress it into a solid. It can effuse through porous solids like a gas, overcoming the mass transfer limitations that slow liquid transport through such materials. SCFs are superior to gases in their ability to dissolve materials like liquids or solids. Near the critical point, small changes in pressure or temperature result in large changes in density, allowing many properties of a supercritical fluid to be "fine-tuned".

Supercritical fluids occur in the atmospheres of the gas giants Jupiter and Saturn, the terrestrial planet Venus, and probably in those of the ice giants Uranus and Neptune. Supercritical water is found on Earth, such as the water issuing from black smokers, a type of hydrothermal vent. SCFs are used as a substitute for organic solvents in a range of industrial and laboratory processes, most commonly carbon dioxide for decaffeination and water for steam boilers for power generation. Some substances are soluble in the supercritical state of a solvent (e.g. carbon dioxide) but insoluble in the gaseous or liquid state—or vice versa. This can be used to extract a substance and transport it elsewhere in solution before depositing it in the desired place by allowing or inducing a phase transition in the solvent.

Properties

Supercritical fluids generally have properties between those of a gas and a liquid. In Table 1, the critical properties are shown for some substances that are commonly used as supercritical fluids.

Table 1. Critical properties of various solvents 
Solvent Molecular mass Critical temperature Critical pressure Critical density
g/mol K MPa (atm) g/cm3
Carbon dioxide (CO2) 44.01 304.1 7.38 (72.8) 0.469
Water (H2O) 18.015 647.096 22.064 (217.755) 0.322
Methane (CH4) 16.04 190.4 4.60 (45.4) 0.162
Ethane (C2H6) 30.07 305.3 4.87 (48.1) 0.203
Propane (C3H8) 44.09 369.8 4.25 (41.9) 0.217
Ethylene (C2H4) 28.05 282.4 5.04 (49.7) 0.215
Propylene (C3H6) 42.08 364.9 4.60 (45.4) 0.232
Methanol (CH3OH) 32.04 512.6 8.09 (79.8) 0.272
Ethanol (C2H5OH) 46.07 513.9 6.14 (60.6) 0.276
Acetone (C3H6O) 58.08 508.1 4.70 (46.4) 0.278
Nitrous oxide (N2O) 44.013 306.57 7.35 (72.5) 0.452

†Source: International Association for Properties of Water and Steam (IAPWS)

Table 2 shows density, diffusivity and viscosity for typical liquids, gases and supercritical fluids.

Table 2. Comparison of gases, supercritical fluids and liquids

Density (kg/m3) Viscosity (μPa·s) Diffusivity (mm2/s)
Gases 1 10 1–10
Supercritical fluids 100–1000 50–100 0.01–0.1
Liquids 1000 500–1000 0.001

Also, there is no surface tension in a supercritical fluid, as there is no liquid/gas phase boundary. By changing the pressure and temperature of the fluid, the properties can be "tuned" to be more liquid-like or more gas-like. One of the most important properties is the solubility of material in the fluid. Solubility in a supercritical fluid tends to increase with density of the fluid (at constant temperature). Since density increases with pressure, solubility tends to increase with pressure. The relationship with temperature is a little more complicated. At constant density, solubility will increase with temperature. However, close to the critical point, the density can drop sharply with a slight increase in temperature. Therefore, close to the critical temperature, solubility often drops with increasing temperature, then rises again.

Mixtures

Typically, supercritical fluids are completely miscible with each other, so that a binary mixture forms a single gaseous phase if the critical point of the mixture is exceeded. However, exceptions are known in systems where one component is much more volatile than the other, which in some cases form two immiscible gas phases at high pressure and temperatures above the component critical points. This behavior has been found for example in the systems N2-NH3, NH3-CH4, SO2-N2 and n-butane-H2O.

The critical point of a binary mixture can be estimated as the arithmetic mean of the critical temperatures and pressures of the two components,

Tc(mix) = χA × Tc(A) + χB × Tc(B)

where χi denotes the mole fraction of component i.

For greater accuracy, the critical point can be calculated using equations of state, such as the Peng–Robinson, or group-contribution methods. Other properties, such as density, can also be calculated using equations of state.

Phase diagram

Figure 1. Carbon dioxide pressure-temperature phase diagram
Figure 2. Carbon dioxide density-pressure phase diagram

Figures 1 and 2 show two-dimensional projections of a phase diagram. In the pressure-temperature phase diagram (Fig. 1) the boiling curve separates the gas and liquid region and ends in the critical point, where the liquid and gas phases disappear to become a single supercritical phase.

The appearance of a single phase can also be observed in the density-pressure phase diagram for carbon dioxide (Fig. 2). At well below the critical temperature, e.g., 280 K, as the pressure increases, the gas compresses and eventually (at just over 40 bar) condenses into a much denser liquid, resulting in the discontinuity in the line (vertical dotted line). The system consists of 2 phases in equilibrium, a dense liquid and a low density gas. As the critical temperature is approached (300 K), the density of the gas at equilibrium becomes higher, and that of the liquid lower. At the critical point, (304.1 K and 7.38 MPa (73.8 bar)), there is no difference in density, and the 2 phases become one fluid phase. Thus, above the critical temperature a gas cannot be liquefied by pressure. At slightly above the critical temperature (310 K), in the vicinity of the critical pressure, the line is almost vertical. A small increase in pressure causes a large increase in the density of the supercritical phase. Many other physical properties also show large gradients with pressure near the critical point, e.g. viscosity, the relative permittivity and the solvent strength, which are all closely related to the density. At higher temperatures, the fluid starts to behave more like an ideal gas, with a more linear density/pressure relationship, as can be seen in Figure 2. For carbon dioxide at 400 K, the density increases almost linearly with pressure.

Many pressurized gases are actually supercritical fluids. For example, nitrogen has a critical point of 126.2 K (−147 °C) and 3.4 MPa (34 bar). Therefore, nitrogen (or compressed air) in a gas cylinder above this pressure is actually a supercritical fluid. These are more often known as permanent gases. At room temperature, they are well above their critical temperature, and therefore behave as a nearly ideal gas, similar to CO2 at 400 K above. However, they cannot be liquified by mechanical pressure unless cooled below their critical temperature, requiring gravitational pressure such as within gas giants to produce a liquid or solid at high temperatures. Above the critical temperature, elevated pressures can increase the density enough that the SCF exhibits liquid-like density and behaviour. At very high pressures, an SCF can be compressed into a solid because the melting curve extends to the right of the critical point in the P/T phase diagram. While the pressure required to compress supercritical CO2 into a solid can be, depending on the temperature, as low as 570 MPa, that required to solidify supercritical water is 14,000 MPa.

The Fisher–Widom line, the Widom line, or the Frenkel line are thermodynamic concepts that allow to distinguish liquid-like and gas-like states within the supercritical fluid.

History

In 1822, Baron Charles Cagniard de la Tour discovered the critical point of a substance in his famous cannon barrel experiments. Listening to discontinuities in the sound of a rolling flint ball in a sealed cannon filled with fluids at various temperatures, he observed the critical temperature. Above this temperature, the densities of the liquid and gas phases become equal and the distinction between them disappears, resulting in a single supercritical fluid phase.

In recent years, a significant effort has been devoted to investigation of various properties of supercritical fluids. Supercritical fluids have found application in a variety of fields, ranging from the extraction of floral fragrance from flowers to applications in food science such as creating decaffeinated coffee, functional food ingredients, pharmaceuticals, cosmetics, polymers, powders, bio- and functional materials, nano-systems, natural products, biotechnology, fossil and bio-fuels, microelectronics, energy and environment. Much of the excitement and interest of the past decade is due to the enormous progress made in increasing the power of relevant experimental tools. The development of new experimental methods and improvement of existing ones continues to play an important role in this field, with recent research focusing on dynamic properties of fluids.

Natural occurrence

Hydrothermal circulation

A black smoker, a type of hydrothermal vent

Hydrothermal circulation occurs within the Earth's crust wherever fluid becomes heated and begins to convect. These fluids are thought to reach supercritical conditions under a number of different settings, such as in the formation of porphyry copper deposits or high temperature circulation of seawater in the sea floor. At mid-ocean ridges, this circulation is most evident by the appearance of hydrothermal vents known as "black smokers". These are large (metres high) chimneys of sulfide and sulfate minerals which vent fluids up to 400 °C. The fluids appear like great black billowing clouds of smoke due to the precipitation of dissolved metals in the fluid. It is likely that at that depth many of these vent sites reach supercritical conditions, but most cool sufficiently by the time they reach the sea floor to be subcritical. One particular vent site, Turtle Pits, has displayed a brief period of supercriticality at the vent site. A further site, Beebe, in the Cayman Trough, is thought to display sustained supercriticality at the vent orifice.[12]

Planetary atmospheres

The atmosphere of Venus is 96.5% carbon dioxide and 3.5% nitrogen. The surface pressure is 9.3 megapascals (1,350 psi) and the surface temperature is 735 K (462 °C; 863 °F), above the critical points of both major constituents and making the surface atmosphere a supercritical fluid.[13]

The interior atmospheres of the Solar System's four giant planets are composed mainly of hydrogen and helium at temperatures well above their critical points. The gaseous outer atmospheres of the gas giants Jupiter and Saturn transition smoothly into the dense liquid interior, while the nature of the transition zones of the ice giants Neptune and Uranus is unknown.[citation needed] Theoretical models of extrasolar planet Gliese 876 d have posited an ocean of pressurized, supercritical fluid water with a sheet of solid high pressure water ice at the bottom.[citation needed]

Applications

Supercritical fluid extraction

The advantages of supercritical fluid extraction (compared with liquid extraction) are that it is relatively rapid because of the low viscosities and high diffusivities associated with supercritical fluids. Alternative solvents to supercritical fluids may be poisonous, flammable or an environmental hazard to a much larger extent than water or carbon dioxide are. The extraction can be selective to some extent by controlling the density of the medium, and the extracted material is easily recovered by simply depressurizing, allowing the supercritical fluid to return to gas phase and evaporate leaving little or no solvent residues. Carbon dioxide is the most common supercritical solvent. It is used on a large scale for the decaffeination of green coffee beans, the extraction of hops for beer production,[14] and the production of essential oils and pharmaceutical products from plants.[15] A few laboratory test methods include the use of supercritical fluid extraction as an extraction method instead of using traditional solvents.[16][17][18]

Supercritical fluid decomposition

Supercritical water can be used to decompose biomass via Supercritical Water Gasification of biomass.[19] This type of biomass gasification can be used to produce hydrocarbon fuels for use in an efficient combustion device or to produce hydrogen for use in a fuel cell. In the latter case, hydrogen yield can be much higher than the hydrogen content of the biomass due to steam reforming where water is a hydrogen-providing participant in the overall reaction.

Dry-cleaning

Supercritical carbon dioxide (SCD) can be used instead of PERC (perchloroethylene) or other undesirable solvents for dry-cleaning. Supercritical carbon dioxide sometimes intercalates into buttons, and, when the SCD is depressurized, the buttons pop, or break apart. Detergents that are soluble in carbon dioxide improve the solvating power of the solvent.[20] CO2-based dry cleaning equipment uses liquid CO2, not supercritical CO2, to avoid damage to the buttons.

Supercritical fluid chromatography

Supercritical fluid chromatography (SFC) can be used on an analytical scale, where it combines many of the advantages of high performance liquid chromatography (HPLC) and gas chromatography (GC). It can be used with non-volatile and thermally labile analytes (unlike GC) and can be used with the universal flame ionization detector (unlike HPLC), as well as producing narrower peaks due to rapid diffusion. In practice, the advantages offered by SFC have not been sufficient to displace the widely used HPLC and GC, except in a few cases such as chiral separations and analysis of high-molecular-weight hydrocarbons.[21] For manufacturing, efficient preparative simulated moving bed units are available.[22] The purity of the final products is very high, but the cost makes it suitable only for very high-value materials such as pharmaceuticals.

Chemical reactions

Changing the conditions of the reaction solvent can allow separation of phases for product removal, or single phase for reaction. Rapid diffusion accelerates diffusion controlled reactions. Temperature and pressure can tune the reaction down preferred pathways, e.g., to improve yield of a particular chiral isomer.[23] There are also significant environmental benefits over conventional organic solvents. Industrial syntheses that are performed at supercritical conditions include those of polyethylene from supercritical ethene, isopropyl alcohol from supercritical propene, 2-butanol from supercritical butene, and ammonia from a supercritical mix of nitrogen and hydrogen.[24] Other reactions were, in the past, performed industrially in supercritical conditions, including the synthesis of methanol and thermal (non-catalytic) oil cracking. Because of the development of effective catalysts, the required temperatures of those two processes have been reduced and are no longer supercritical.[24]

Impregnation and dyeing

Impregnation is, in essence, the converse of extraction. A substance is dissolved in the supercritical fluid, the solution flowed past a solid substrate, and is deposited on or dissolves in the substrate. Dyeing, which is readily carried out on polymer fibres such as polyester using disperse (non-ionic) dyes, is a special case of this. Carbon dioxide also dissolves in many polymers, considerably swelling and plasticising them and further accelerating the diffusion process.

Nano and micro particle formation

The formation of small particles of a substance with a narrow size distribution is an important process in the pharmaceutical and other industries. Supercritical fluids provide a number of ways of achieving this by rapidly exceeding the saturation point of a solute by dilution, depressurization or a combination of these. These processes occur faster in supercritical fluids than in liquids, promoting nucleation or spinodal decomposition over crystal growth and yielding very small and regularly sized particles. Recent supercritical fluids have shown the capability to reduce particles up to a range of 5-2000 nm.

Generation of pharmaceutical cocrystals

Supercritical fluids act as a new medium for the generation of novel crystalline forms of APIs (Active Pharmaceutical Ingredients) named as pharmaceutical cocrystals. Supercritical fluid technology offers a new platform that allows a single-step generation of particles that are difficult or even impossible to obtain by traditional techniques. The generation of pure and dried new cocrystals (crystalline molecular complexes comprising the API and one or more conformers in the crystal lattice) can be achieved due to unique properties of SCFs by using different supercritical fluid properties: supercritical CO2 solvent power, anti-solvent effect and its atomization enhancement.

Supercritical drying

Supercritical drying is a method of removing solvent without surface tension effects. As a liquid dries, the surface tension drags on small structures within a solid, causing distortion and shrinkage. Under supercritical conditions there is no surface tension, and the supercritical fluid can be removed without distortion. Supercritical drying is used in the manufacturing process of aerogels and drying of delicate materials such as archaeological samples and biological samples for electron microscopy.

Supercritical water electrolysis

Electrolysis of water in a supercritical state, reduces the overpotentials found in other electrolysers, thereby improving the electrical efficiency of the production of oxygen and hydrogen.

Increased temperature reduces thermodynamic barriers and increases kinetics. No bubbles of oxygen or hydrogen are formed on the electrodes, therefore no insulating layer is formed between catalyst and water, reducing the ohmic losses. The gas-like properties provide rapid mass transfer.

Supercritical water oxidation

Supercritical water oxidation uses supercritical water as a medium in which to oxidize hazardous waste, eliminating production of toxic combustion products that burning can produce.

The waste product to be oxidised is dissolved in the supercritical water along with molecular oxygen (or an oxidising agent that gives up oxygen upon decomposition, e.g. hydrogen peroxide) at which point the oxidation reaction occurs.

Supercritical water hydrolysis

Supercritical hydrolysis is a method of converting all biomass polysaccharides as well the associated lignin into low molecular compounds by contacting with water alone under supercritical conditions. The supercritical water, acts as a solvent, a supplier of bond-breaking thermal energy, a heat transfer agent and as a source of hydrogen atoms. All polysaccharides are converted into simple sugars in near-quantitative yield in a second or less. The aliphatic inter-ring linkages of lignin are also readily cleaved into free radicals that are stabilized by hydrogen originating from the water. The aromatic rings of the lignin are unaffected under short reaction times so that the lignin-derived products are low molecular weight mixed phenols. To take advantage of the very short reaction times needed for cleavage a continuous reaction system must be devised. The amount of water heated to a supercritical state is thereby minimized.

Supercritical water gasification

Supercritical water gasification is a process of exploiting the beneficial effect of supercritical water to convert aqueous biomass streams into clean water and gases like H2, CH4, CO2, CO etc.

Supercritical fluid in power generation

The efficiency of a heat engine is ultimately dependent on the temperature difference between heat source and sink (Carnot cycle). To improve efficiency of power stations the operating temperature must be raised. Using water as the working fluid, this takes it into supercritical conditions. Efficiencies can be raised from about 39% for subcritical operation to about 45% using current technology. Many coal-fired supercritical steam generators are operational all over the world. Supercritical carbon dioxide is also proposed as a working fluid, which would have the advantage of lower critical pressure than water, but issues with corrosion are not yet fully solved. One proposed application is the Allam cycle.

Supercritical water reactors (SCWRs) are proposed advanced nuclear systems that offer similar thermal efficiency gains.

Biodiesel production

Conversion of vegetable oil to biodiesel is via a transesterification reaction, where a triglyceride is converted to the methyl esters (of the fatty acids) plus glycerol. This is usually done using methanol and caustic or acid catalysts, but can be achieved using supercritical methanol without a catalyst. The method of using supercritical methanol for biodiesel production was first studied by Saka and his coworkers. This has the advantage of allowing a greater range and water content of feedstocks (in particular, used cooking oil), the product does not need to be washed to remove catalyst, and is easier to design as a continuous process.

Enhanced oil recovery and carbon capture and storage

Supercritical carbon dioxide is used to enhance oil recovery in mature oil fields. At the same time, there is the possibility of using "clean coal technology" to combine enhanced recovery methods with carbon sequestration. The CO2 is separated from other flue gases, compressed to the supercritical state, and injected into geological storage, possibly into existing oil fields to improve yields.

At present, only schemes isolating fossil CO2 from natural gas actually use carbon storage, (e.g., Sleipner gas field), but there are many plans for future CCS schemes involving pre- or post- combustion CO2. There is also the possibility to reduce the amount of CO2 in the atmosphere by using biomass to generate power and sequestering the CO2 produced.

Enhanced geothermal system

The use of supercritical carbon dioxide, instead of water, has been examined as a geothermal working fluid.

Refrigeration

Supercritical carbon dioxide is also emerging as a useful high-temperature refrigerant, being used in new, CFC/HFC-free domestic heat pumps making use of the transcritical cycle. These systems are undergoing continuous development with supercritical carbon dioxide heat pumps already being successfully marketed in Asia. The EcoCute systems from Japan are some of the first commercially successful high-temperature domestic water heat pumps.

Supercritical fluid deposition

Supercritical fluids can be used to deposit functional nanostructured films and nanometer-size particles of metals onto surfaces. The high diffusivities and concentrations of precursor in the fluid as compared to the vacuum systems used in chemical vapour deposition allow deposition to occur in a surface reaction rate limited regime, providing stable and uniform interfacial growth. This is crucial in developing more powerful electronic components, and metal particles deposited in this way are also powerful catalysts for chemical synthesis and electrochemical reactions. Additionally, due to the high rates of precursor transport in solution, it is possible to coat high surface area particles which under chemical vapour deposition would exhibit depletion near the outlet of the system and also be likely to result in unstable interfacial growth features such as dendrites. The result is very thin and uniform films deposited at rates much faster than atomic layer deposition, the best other tool for particle coating at this size scale.

Antimicrobial properties

CO2 at high pressures has antimicrobial properties. While its effectiveness has been shown for various applications, the mechanisms of inactivation have not been fully understood although they have been investigated for more than 60 years.

Ice giant

From Wikipedia, the free encyclopedia
Uranus photographed by Voyager 2 in January 1986
 
Neptune photographed by Voyager 2 in August 1989

An ice giant is a giant planet composed mainly of elements heavier than hydrogen and helium, such as oxygen, carbon, nitrogen, and sulfur. There are two ice giants in the Solar System: Uranus and Neptune.

In astrophysics and planetary science the term "ice" refers to volatile chemical compounds with freezing points above about 100 K, such as water, ammonia, or methane, with freezing points of 273 K (0 °C), 195 K (−78 °C), and 91 K (−182 °C), respectively (see Volatiles). In the 1990s, it was determined that Uranus and Neptune were a distinct class of giant planet, separate from the other giant planets, Jupiter and Saturn, which are gas giants predominantly composed of hydrogen and helium.

Neptune and Uranus are now referred to as ice giants. Lacking well-defined solid surfaces, they are primarily composed of gases and liquids. Their constituent compounds were solids when they were primarily incorporated into the planets during their formation, either directly in the form of ice or trapped in water ice. Today, very little of the water in Uranus and Neptune remains in the form of ice. Instead, water primarily exists as supercritical fluid at the temperatures and pressures within them. Uranus and Neptune consist of only about 20% hydrogen and helium by mass, compared to the Solar System's gas giants, Jupiter and Saturn, which are more than 90% hydrogen and helium by mass.

Terminology

In 1952, science fiction writer James Blish coined the term gas giant and it was used to refer to the large non-terrestrial planets of the Solar System. However, since the late 1940s the compositions of Uranus and Neptune have been understood to be significantly different from those of Jupiter and Saturn. They are primarily composed of elements heavier than hydrogen and helium, forming a separate type of giant planet altogether. Because during their formation Uranus and Neptune incorporated their material as either ice or gas trapped in water ice, the term ice giant came into use. In the early 1970s, the terminology became popular in the science fiction community, e.g., Bova (1971), but the earliest scientific usage of the terminology was likely by Dunne & Burgess (1978) in a NASA report.

Formation

Modelling the formation of terrestrial and gas giants is relatively straightforward and uncontroversial. The terrestrial planets of the Solar System are widely understood to have formed through collisional accumulation of planetesimals within the protoplanetary disk. The gas giantsJupiter, Saturn, and their extrasolar counterpart planets—are thought to have formed solid cores of around 10 Earth masses (ME) through the same process, while accreting gaseous envelopes from the surrounding solar nebula over the course of a few to several million years (Ma), although alternative models of core formation based on pebble accretion have recently been proposed. Some extrasolar giant planets may instead have formed via gravitational disk instabilities.

The formation of Uranus and Neptune through a similar process of core accretion is far more problematic. The escape velocity for the small protoplanets about 20 astronomical units (AU) from the center of the Solar System would have been comparable to their relative velocities. Such bodies crossing the orbits of Saturn or Jupiter would have been liable to be sent on hyperbolic trajectories ejecting them from the system. Such bodies, being swept up by the gas giants, would also have been likely to just be accreted into larger planets or thrown into cometary orbits.

Despite the trouble modelling their formation, many ice giant candidates have been observed orbiting other stars since 2004. This indicates that they may be common in the Milky Way.

Migration

Considering the orbital challenges of protoplanets 20 AU or more from the centre of the Solar System would experience, a simple solution is that the ice giants formed between the orbits of Jupiter and Saturn before being gravitationally scattered outward to their now more distant orbits.

Disk instability

Gravitational instability of the protoplanetary disk could also produce several gas giant protoplanets out to distances of up to 30 AU. Regions of slightly higher density in the disk could lead to the formation of clumps that eventually collapse to planetary densities. A disk with even marginal gravitational instability could yield protoplanets between 10 and 30 AU in over one thousand years (ka). This is much shorter than the 100,000 to 1,000,000 years required to produce protoplanets through core accretion of the cloud and could make it viable in even the shortest-lived disks, which exist for only a few million years.

A problem with this model is determining what kept the disk stable before the instability. There are several possible mechanisms allowing gravitational instability to occur during disk evolution. A close encounter with another protostar could provide a gravitational kick to an otherwise stable disk. A disk evolving magnetically is likely to have magnetic dead zones, due to varying degrees of ionization, where mass moved by magnetic forces could pile up, eventually becoming marginally gravitationally unstable. A protoplanetary disk may simply accrete matter slowly, causing relatively short periods of marginal gravitational instability and bursts of mass collection, followed by periods where the surface density drops below what is required to sustain the instability.

Photoevaporation

Observations of photoevaporation of protoplanetary disks in the Orion Trapezium Cluster by extreme ultraviolet (EUV) radiation emitted by θ1 Orionis C suggests another possible mechanism for the formation of ice giants. Multiple-Jupiter-mass gas-giant protoplanets could have rapidly formed due to disk instability before having most of their hydrogen envelopes stripped off by intense EUV radiation from a nearby massive star.

In the Carina Nebula, EUV fluxes are approximately 100 times higher than in Trapezium's Orion Nebula. Protoplanetary disks are present in both nebulae. Higher EUV fluxes make this an even more likely possibility for ice-giant formation. The stronger EUV would increase the removal of the gas envelopes from protoplanets before they could collapse sufficiently to resist further loss.

Characteristics

These cut-aways illustrate interior models of the giant planets. The planetary cores of gas giants Jupiter and Saturn are overlaid by a deep layer of metallic hydrogen, whereas the mantles of the ice giants Uranus and Neptune are composed of heavier elements.

The ice giants represent one of two fundamentally different categories of giant planets present in the Solar System, the other group being the more-familiar gas giants, which are composed of more than 90% hydrogen and helium (by mass). Their hydrogen is thought to extend all the way down to their small rocky cores, where hydrogen molecular ion transitions to metallic hydrogen under the extreme pressures of hundreds of gigapascals (GPa).

The ice giants are primarily composed of heavier elements. Based on the abundance of elements in the universe, oxygen, carbon, nitrogen, and sulfur are most likely. Although the ice giants also have hydrogen envelopes, these are much smaller. They account for less than 20% of their mass. Their hydrogen also never reaches the depths necessary for the pressure to create metallic hydrogen. These envelopes nevertheless limit observation of the ice giants' interiors, and thereby the information on their composition and evolution.

Although Uranus and Neptune are referred to as ice giant planets, it is thought that there is a supercritical water-ammonia ocean beneath their clouds, which accounts for about two-thirds of their total mass.

Atmosphere and weather

The gaseous outer layers of the ice giants have several similarities to those of the gas giants. These include long-lived, high-speed equatorial winds, polar vortices, large-scale circulation patterns, and complex chemical processes driven by ultraviolet radiation from above and mixing with the lower atmosphere.

Studying the ice giants' atmospheric patterns also gives insights into atmospheric physics. Their compositions promote different chemical processes and they receive far less sunlight in their distant orbits than any other planets in the Solar System (increasing the relevance of internal heating on weather patterns).

The largest visible feature on Neptune is the recurring Great Dark Spot. It forms and dissipates every few years, as opposed to the similarly sized Great Red Spot of Jupiter, which has persisted for centuries. Of all known giant planets in the Solar System, Neptune emits the most internal heat per unit of absorbed sunlight, a ratio of approximately 2.6. Saturn, the next-highest emitter, only has a ratio of about 1.8. Uranus emits the least heat, one-tenth as much as Neptune. It is suspected that this may be related to its extreme 98˚ axial tilt. This causes its seasonal patterns to be very different from those of any other planet in the Solar System.

There are still no complete models explaining the atmospheric features observed in the ice giants. Understanding these features will help elucidate how the atmospheres of giant planets in general function. Consequently, such insights could help scientists better predict the atmospheric structure and behaviour of giant exoplanets discovered to be very close to their host stars (pegasean planets) and exoplanets with masses and radii between that of the giant and terrestrial planets found in the Solar System.

Interior

Because of their large sizes and low thermal conductivities, the planetary interior pressures range up to several hundred GPa and temperatures of several thousand kelvins (K).

In March 2012, it was found that the compressibility of water used in ice-giant models could be off by one-third. This value is important for modeling ice giants, and has a ripple effect on understanding them.

Magnetic fields

The magnetic fields of Uranus and Neptune are both unusually displaced and tilted. Their field strengths are intermediate between those of the gas giants and those of the terrestrial planets, being 50 and 25 times that of Earth's, respectively. The equatorial magnetic field strengths of Uranus and Neptune are respectively 75 percent and 45 percent of Earth's 0.305 gauss. Their magnetic fields are believed to originate in an ionized convecting fluid-ice mantle.

Spacecraft visitation

Past

Proposals

Human interactome

From Wikipedia, the free encyclopedia
https://en.wikipedia.org/wiki/Human_interactome

The human interactome is the set of protein–protein interactions (the interactome) that occur in human cells.The sequencing of reference genomes, in particular the Human Genome Project, has revolutionized human genetics, molecular biology, and clinical medicine. Genome-wide association study results have led to the association of genes with most Mendelian disorders, and over 140 000 germline mutations have been associated with at least one genetic disease. However, it became apparent that inherent to these studies is an emphasis on clinical outcome rather than a comprehensive understanding of human disease; indeed to date the most significant contributions of GWAS have been restricted to the “low-hanging fruit” of direct single mutation disorders, prompting a systems biology approach to genomic analysis. The connection between genotype and phenotype (how variation in genotype affects the disease or normal functioning of the cell and the human body) remain elusive, especially in the context of multigenic complex traits and cancer. To assign functional context to genotypic changes, much of recent research efforts have been devoted to the mapping of the networks formed by interactions of cellular and genetic components in humans, as well as how these networks are altered by genetic and somatic disease.

Background

With the sequencing of the genomes of a diverse array or model organisms, it became clear that the number of genes does not correlate with the human perception of relative organism complexity – the human proteome contains some 20 000 genes, which is smaller than some species such as corn. A statistical approach to calculating the number of interactions in humans gives an estimate of around 650 000, one order of magnitude bigger than Drosophila and 3 times larger than C. Elegans. As of 2008, only about <0.3% of all estimated interactions among human proteins has been identified, although in recent years there has been exponential growth in discovery – as of 2015, over 210 000 unique human positive protein–protein interactions are currently catalogued, and bioGRID database contains almost 750 000 literature-curated PPI's for 30 model organisms, 300 000 of which are verified or predicted human physical or genetic protein–protein interactions, a 50% increase from 2013. The currently available information on the human interactome network originates from either literature-curated interactions, high-throughput experiments, or from potential interactions predicted from interactome data, whether through phylogenetic profiling (evolutionary similarity), statistical network inference, or text/literature mining methods.

Protein–protein interactions are only the raw material for networks. To form useful interactome databases and create integrated networks, other types of data that can be combined with protein–protein interactions include information on gene expression and co-expression, cellular co-localization of proteins (based on microscopy), genetic information, metabolic and signalling pathways, and more. The end goal of unravelling human protein interactomes is ultimately to understand mechanisms of disease and uncover previously unknown disease genes. It has been found that proteins with a high number of interactions (outward edges) are significantly more likely to be hubs in modules that correlate with disease, probably because proteins with more interactions are involved in more biological functions. By mapping disease alterations to the human interactome, we can gain a much better understanding of the pathways and biological processes of disease.

Studying the human interactome

Analysis of metabolic networks of proteins hearkens back to the 1940s, but it was not until the late 1990s and early 2000s that computational data-driven genomic analyses to predict functional context and networks of genetic associations appeared in earnest. Since then, the interactomes of many model organisms are considered to have been well characterized, notably the Saccharomyces cerevisiae Interactome and the Drosophila interactome.

High throughput experimental approaches for discovering protein–protein interactions typically perform a version of the two-hybrid screening approach or tandem affinity purification followed by mass spectrometry. Information from experiments and literature curation are compiled into databases of protein interactions, such as DIP, and BioGRID. A more recent effort, HINT-KB, attempts to amalgamate most of the current PPI databases, but filtering systematically erroneous interactions as well as trying to correct for inherent sociological sampling biases in literature curated datasets.

Smaller human interactome networks have been described in the specific context of important drivers of many different disorders, including neurodegenerative disorders, autism and other psychiatric disorders, and cancer. Cancer gene networks have been particularly well studied, due in part to large genome initiatives such as The Cancer Genome Atlas (TCGA). A large portion of the mutational landscape including intra-tumoural heterogeneity has been mapped for most common types of cancers  (for example, breast cancer has been well studied), and many studies have also investigated the difference between active driver genes and passive passenger mutations in the context of cancer interaction networks.

The first attempts at large-scale integrative human interactome mapping occurred around 2005. Stetzl et al. used a protein matrix of 4500 baits and 5600 preys in a yeast two hybrid system to piece together the interactome, and Rual et al. performed a similar yeast-two hybrid study verified with co-affinity purification and correlation with other biological attributes, revealing more than 300 connections to 100 disease-associated proteins. Since those pioneering efforts, hundreds of similar studies have been conducted. Compiled databases such as UniHI provide platform for single entry. Futschik et al. performed a meta analysis of eight interactome maps and found that of 57 000 interacting proteins in total, there was a small (albeit statistically significant) overlap between the different databases, indicating considerable selection and detection biases.

In 2010, around 130 000 binary interactions in the interactome were described in the most popular databases, but many were verified with only one source. With the rapid development of high throughput methods, datasets still suffer from high rates of false positives and low coverage of the interactome. Tyagi et al. described a novel framework for incorporating structural complexes and binding interfaces for verification. This was part of much larger efforts for PPI verification; interaction networks are typically validated further by using a combination of coexpression profiles, protein structural information, Gene ontology terms, topological considerations, and colocalization before being considered “high-confidence”.

A recent resource paper (November 2014)  attempts to provide a more comprehensive proteome level map of the human interactome. It found vast uncharted territory in the human interactome, and used diverse methods to build a new interactome map correcting for curation bias, including probing all pairwise combinations of 13 000 protein products for interaction using Yeast two hybrid and co-affinity purification, in a massive coordinated effort across research labs in Canada and the United States. However, this still represents confirmation of but a fraction of expected interactions – around 30 000 of high confidence. Despite the coordinated efforts of many, the human interactome is still very much a work in progress.

Möbius strip

From Wikipedia, the free encyclopedia https://en.wikipedia.org/wiki/M%C3%B6bius_strip   A Möbius stri...