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Thursday, April 18, 2019

History of Libya under Muammar Gaddafi

From Wikipedia, the free encyclopedia

The green flag of the Libyan Arab Jamahiriya was the only national flag in the world with just one colour and no other features: the colour green, which represented Islam and Gaddafi's Third International Theory, outlined in The Green Book.
 
Gaddafi at the 12th African Union conference in 2009
 
Muammar Gaddafi became the de facto leader of Libya on 1 September 1969 after leading a group of young Libyan military officers against King Idris I in a bloodless coup d'état. After the king had fled the country, the Libyan Revolutionary Command Council (RCC) headed by Gaddafi abolished the monarchy and the old constitution and established the Libyan Arab Republic, with the motto "freedom, socialism and unity".

After coming to power, the RCC government initiated a process of directing funds toward providing education, health care and housing for all. Public education in the country became free and primary education compulsory for both sexes. Medical care became available to the public at no cost, but providing housing for all was a task the RCC government was not able to complete. Under Gaddafi, per capita income in the country rose to more than US $11,000, the fifth highest in Africa. The increase in prosperity was accompanied by a controversial foreign policy, and there was increased domestic political repression.

During the 1980s and 1990s, Gaddafi, in alliance with the Eastern Bloc and Fidel Castro's Cuba, openly supported rebel movements like Nelson Mandela's African National Congress, the Palestine Liberation Organization, the Irish Republican Army and the Polisario Front (Western Sahara). Gaddafi's government was either known to be or suspected of participating in or aiding terrorist acts by these and other proxy forces. Additionally, Gaddafi undertook several invasions of neighboring states in Africa, notably Chad in the 1970s and 1980s. All of his actions led to a deterioration of Libya's foreign relations with several countries, mostly Western states, and culminated in the 1986 United States bombing of Libya. Gaddafi defended his government's actions by citing the need to support anti-imperialist and anti-colonial movements around the world. Notably, Gaddafi supported anti-Zionist, pan-Africanist and black civil rights movements. Gaddafi's behavior, often erratic, led some outsiders to conclude that he was not mentally sound, a claim disputed by the Libyan authorities and other observers close to Gaddafi. Despite receiving extensive aid and technical assistance from the Soviet Union and its allies, Gaddafi retained close ties to pro-American governments in Western Europe, largely by incentivising Western oil companies with promises of access to lucrative Libyan energy sectors. After the 9/11 attacks, strained relations between Libya and the West were mostly normalised, and sanctions against the country relaxed, in exchange for Libyan efforts to shrink its nuclear program

In early 2011, a civil war broke out in the context of the wider "Arab Spring". The rebel anti-Gaddafi forces formed a committee named the National Transitional Council on 27 February 2011. It was meant to act as an interim authority in the rebel-controlled areas. After killings by government forces in addition to those by the rebel forces, a multinational coalition led by NATO forces intervened on 21 March 2011 in support of the rebels. The International Criminal Court issued an arrest warrant against Gaddafi and his entourage on 27 June 2011. Gaddafi's government was overthrown in the wake of the fall of Tripoli to the rebel forces on 20 August 2011, although pockets of resistance held by forces in support of Gaddafi's government held out for another two months, especially in Gaddafi's hometown of Sirte, which he declared the new capital of Libya on 1 September 2011. The fall of the last remaining cities under pro-Gaddafi control and Sirte's capture on 20 October 2011, followed by the subsequent killing of Gaddafi, marked the end of the Libyan Arab Jamahiriya.

The name of Libya was changed several times during Gaddafi's tenure as leader. At first, the name was the Libyan Arab Republic. In 1977, the name was changed to Socialist People's Libyan Arab Jamahiriya. Jamahiriya was a term coined by Gaddafi, usually translated as "state of the masses". The country was renamed again in 1986 as the Great Socialist People's Libyan Arab Jamahiriya, after the 1986 United States bombing of Libya.

Coup d'état of 1969

The discovery of significant oil reserves in 1959 and the subsequent income from petroleum sales enabled the Kingdom of Libya to transition from one of the world's poorest nations to a wealthy state. Although oil drastically improved the Libyan government's finances, resentment began to build over the increased concentration of the nation's wealth in the hands of the noble King Idris. This discontent mounted with the rise of Nasserism and Arab nationalism/socialism throughout North Africa and the Middle East.

On 1 September 1969, a group of about 70 young army officers known as the Free Officers Movement and enlisted men mostly assigned to the Signal Corps, seized control of the government and in a stroke abolished the Libyan monarchy. The coup was launched at Benghazi, and within two hours the takeover was completed. Army units quickly rallied in support of the coup, and within a few days firmly established military control in Tripoli and elsewhere throughout the country. Popular reception of the coup, especially by younger people in the urban areas, was enthusiastic. Fears of resistance in Cyrenaica and Fezzan proved unfounded. No deaths or violent incidents related to the coup were reported.

The Free Officers Movement, which claimed credit for carrying out the coup, was headed by a twelve-member directorate that designated itself the Revolutionary Command Council (RCC). This body constituted the Libyan government after the coup. In its initial proclamation on 1 September, the RCC declared the country to be a free and sovereign state called the Libyan Arab Republic, which would proceed "in the path of freedom, unity, and social justice, guaranteeing the right of equality to its citizens, and opening before them the doors of honorable work." The rule of the Turks and Italians and the "reactionary" government just overthrown were characterized as belonging to "dark ages", from which the Libyan people were called to move forward as "free brothers" to a new age of prosperity, equality, and honor. 

The RCC advised diplomatic representatives in Libya that the revolutionary changes had not been directed from outside the country, that existing treaties and agreements would remain in effect, and that foreign lives and property would be protected. Diplomatic recognition of the new government came quickly from countries throughout the world. United States recognition was officially extended on 6 September.

Post-coup

Gaddafi (left) with Egyptian President Gamal Abdel Nasser in 1969
 
In view of the lack of internal resistance, it appeared that the chief danger to the new government lay in the possibility of a reaction inspired by the absent King Idris or his designated heir, Hasan Al-Rida, who had been taken into custody at the time of the coup along with other senior civil and military officials of the royal government. Within days of the coup, however, Hasan publicly renounced all rights to the throne, stated his support for the new government, and called on the people to accept it without violence.

Idris, in an exchange of messages with the RCC through Egypt's President Nasser, dissociated himself from reported attempts to secure British intervention and disclaimed any intention of coming back to Libya. In return, he was assured by the RCC of the safety of his family still in the country. At his own request and with Nasser's approval, Idris took up residence once again in Egypt, where he had spent his first exile and where he remained until his death in 1983. 

On 7 September 1969, the RCC announced that it had appointed a cabinet to conduct the government of the new republic. An American-educated technician, Mahmud Sulayman al-Maghribi, who had been imprisoned since 1967 for his political activities, was designated prime minister. He presided over the eight-member Council of Ministers, of whom six, like Maghrabi, were civilians and two – Adam Said Hawwaz and Musa Ahmad – were military officers. Neither of the officers was a member of the RCC. 

The Council of Ministers was instructed to "implement the state's general policy as drawn up by the RCC", leaving no doubt where ultimate authority rested. The next day the RCC decided to promote Captain Gaddafi to colonel and to appoint him commander in chief of the Libyan Armed Forces. Although RCC spokesmen declined until January 1970 to reveal any other names of RCC members, it was apparent from that date onward that the head of the RCC and new de facto head of state was Gaddafi.

Analysts were quick to point out the striking similarities between the Libyan military coup of 1969 and that in Egypt under Nasser in 1952, and it became clear that the Egyptian experience and the charismatic figure of Nasser had formed the model for the Free Officers Movement. As the RCC in the last months of 1969 moved vigorously to institute domestic reforms, it proclaimed neutrality in the confrontation between the superpowers and opposition to all forms of colonialism and "imperialism". It also made clear Libya's dedication to Arab unity and to the support of the Palestinian cause against Israel.

The RCC reaffirmed the country's identity as part of the "Arab nation" and its state religion as Islam. It abolished parliamentary institutions, all legislative functions being assumed by the RCC, and continued the prohibition against political parties, in effect since 1952. The new government categorically rejected communism – in large part because it was atheist – and officially espoused an Arab interpretation of socialism that integrated Islamic principles with social, economic, and political reform. Libya had shifted, virtually overnight, from the camp of conservative Arab traditionalist states to that of the radical nationalist states.

Libyan Arab Republic (1969–1977)

Libyan Arab Republic

الجمهورية العربية الليبية
Al-Jumhūrīyah Al-ʿArabiyyah Al-Lībiyyah  (in Arabic)
Repubblica Araba Libica  (in Italian)
1969–1977
Anthem: الله أكبر
Allahu Akbar

God is the Greatest
Location of Libya
CapitalTripoli
Common languagesArabic
Italian
GovernmentOne-party state under a military dictatorship
Chairman of the Revolutionary Command Council 

• 1969–1977
Muammar Gaddafi
Historical eraCold War

1 September 1969
• Jamahiriya established
2 March 1977
Population

• 1977
2,681,900
CurrencyLibyan dinar
Calling code218
ISO 3166 codeLY
Preceded by Succeeded by
Kingdom of Libya
Great Socialist People's Libyan Arab Jamahiriya

Attempted counter-coups

Following the formation of the Libyan Arab Republic, Gaddafi and his associates insisted that their government would not rest on individual leadership, but rather on collegial decision making.

The first major cabinet change occurred soon after the first challenge to the government. In December 1969, Adam Said Hawwaz, the minister of defense, and Musa Ahmad, the minister of interior, were arrested and accused of planning a coup. In the new cabinet formed after the crisis, Gaddafi, retaining his post as chairman of the RCC, also became prime minister and defense minister.

Major Abdel Salam Jallud, generally regarded as second only to Gaddafi in the RCC, became deputy prime minister and minister of interior. This cabinet totaled thirteen members, of whom five were RCC officers. The government was challenged a second time in July 1970 when Abdullah Abid Sanusi and Ahmed al-Senussi, distant cousins of former King Idris, and members of the Sayf an Nasr clan of Fezzan were accused of plotting to seize power for themselves. After the plot was foiled, a substantial cabinet change occurred, RCC officers for the first time forming a majority among new ministers.

Assertion of Gaddafi's control

From the start, RCC spokesmen had indicated a serious intent to bring the "defunct regime" to account. In 1971 and 1972 more than 200 former government officials—including seven prime ministers and numerous cabinet ministers—as well as former King Idris and members of the royal family, were brought to trial on charges of treason and corruption in the Libyan People's Court

Many, who like Idris lived in exile, were tried in absentia. Although a large percentage of those charged were acquitted, sentences of up to fifteen years in prison and heavy fines were imposed on others. Five death sentences, all but one of them in absentia, were pronounced, among them, one against Idris. Fatima, the former queen, and Hasan ar Rida were sentenced to five and three years in prison, respectively. 

Meanwhile, Gaddafi and the RCC had disbanded the Sanusi order and officially downgraded its historical role in achieving Libya's independence. He also attacked regional and tribal differences as obstructions in the path of social advancement and Arab unity, dismissing traditional leaders and drawing administrative boundaries across tribal groupings

The Free Officers Movement was renamed "Arab Socialist Union" (ASU) in 1971, modeled after Egypt's Arab Socialist Union, and made the sole legal party in Gaddafi's Libya. It acted as a "vehicle of national expression", purporting to "raise the political consciousness of Libyans" and to "aid the RCC in formulating public policy through debate in open forums". Trade unions were incorporated into the ASU and strikes outlawed. The press, already subject to censorship, was officially conscripted in 1972 as an agent of the revolution. Italians and what remained of the Jewish community were expelled from the country and their property confiscated in October 1970. 

Flag of Libya (1969–1972)
 
Coat of arms of Libya (1969–1972)
 
In 1972, Libya joined the Federation of Arab Republics with Egypt and Syria but the intended union of pan-Arabic states never had the intended success, and was effectively dormant after 1973.

As months passed, Gaddafi, caught up in his apocalyptic visions of revolutionary Pan-Arabism and Islam locked in mortal struggle with what he termed the encircling, demonic forces of reaction, imperialism, and Zionism, increasingly devoted attention to international rather than internal affairs. As a result, routine administrative tasks fell to Major Jallud, who in 1972 became prime minister in place of Gaddafi. Two years later Jallud assumed Gaddafi's remaining administrative and protocol duties to allow Gaddafi to devote his time to revolutionary theorizing. Gaddafi remained commander in chief of the armed forces and effective head of state. The foreign press speculated about an eclipse of his authority and personality within the RCC, but Gaddafi soon dispelled such theories by his measures to restructure Libyan society.

Alignment with the Soviet bloc

After the September coup, U.S. forces proceeded deliberately with the planned withdrawal from Wheelus Air Base under the agreement made with the previous government. The last of the American contingent turned the facility over to the Libyans on 11 June 1970, a date thereafter celebrated in Libya as a national holiday.

As relations with the U.S. steadily deteriorated, Gaddafi forged close links with the Soviet Union and other Eastern Bloc countries, all the while maintaining Libya's stance as a nonaligned country and opposing the spread of communism in the Arab world. Libya's army—sharply increased from the 6,000-man prerevolutionary force that had been trained and equipped by the British—was armed with Soviet-built armor and missiles.

Petroleum politics

The economic base for Libya's revolution has been its oil revenues. However, Libya's petroleum reserves were small compared with those of other major Arab petroleum-producing states. As a consequence, Libya was more ready to ration output in order to conserve its natural wealth and less responsive to moderating its price-rise demands than the other countries. Petroleum was seen both as a means of financing the economic and social development of a woefully underdeveloped country and as a political weapon to brandish in the Arab struggle against Israel.

The increase in production that followed the 1969 revolution was accompanied by Libyan demands for higher petroleum prices, a greater share of revenues, and more control over the development of the country's petroleum industry. Foreign petroleum companies agreed to a price hike of more than three times the going rate (from US$0.90 to US$3.45 per barrel) early in 1971. In December, the Libyan government suddenly nationalized the holdings of British Petroleum in Libya and withdrew funds amounting to approximately US$550 million invested in British banks as a result of a foreign policy dispute. British Petroleum rejected as inadequate a Libyan offer of compensation, and the British treasury banned Libya from participation in the sterling area.

In 1973, the Libyan government announced the nationalization of a controlling interest in all other petroleum companies operating in the country. This step gave Libya control of about 60 percent of its domestic oil production by early 1974, a figure that subsequently rose to 70 percent. Total nationalization was out of the question, given the need for foreign expertise and funds in oil exploration, production, and distribution.

1973 oil crisis

Insisting on the continued use of petroleum as leverage against Israel and its supporters in the West, Libya strongly urged the Organization of Petroleum Exporting Countries (OPEC) to take action in 1973, and Libyan militancy was partially responsible for OPEC measures to raise oil prices, impose embargoes, and gain control of production. On 19 October 1973, Libya was the first Arab nation to issue an oil embargo against the United States after US President Richard Nixon announced the US would provide Israel with a $2.2 billion military aid program during the Yom Kippur War. Saudi Arabia and other Arab oil producing nations in OPEC would follow suit the next day.

While the other Arab nations lifted their oil embargoes on 18 March 1974, the Gaddafi regime refused to do so. As a consequence of such policies, Libya's oil production declined by half between 1970 and 1974, while revenues from oil exports more than quadrupled. Production continued to fall, bottoming out at an eleven-year low in 1975 at a time when the government was preparing to invest large amounts of petroleum revenues in other sectors of the economy. Thereafter, output stabilized at about two million barrels per day. Production and hence income declined yet again in the early 1980s because of the high price of Libyan crude and because recession in the industrialized world reduced demand for oil from all sources.

Libya's Five-Year Economic and Social Transformation Plan (1976–80), announced in 1975, was programmed to pump US$20 billion into the development of a broad range of economic activities that would continue to provide income after Libya's petroleum reserves had been exhausted. Agriculture was slated to receive the largest share of aid in an effort to make Libya self-sufficient in food and to help keep the rural population on the land. Industry, of which there was little before the revolution, also received a significant amount of funding in the first development plan as well as in the second, launched in 1981.

Transition to the Jamahiriya (1973–1977)

(Alfateh, 1 September 1969) Festivity Alfateh in Bayda, Libya, on 1 September 2010.

The "remaking of Libyan society" contained in Gaddafi's ideological visions began to be put into practice formally in 1973, with a so-called cultural or popular revolution. This revolution was designed to create bureaucratic efficiency, public interest and participation in the subnational governmental system, and national political coordination. In an attempt to instill revolutionary fervor into his compatriots and to involve large numbers of them in political affairs, Gaddafi urged them to challenge traditional authority and to take over and run government organs themselves. The instrument for doing this was the people's committee. Within a few months, such committees were found all across Libya. They were functionally and geographically based, and eventually became responsible for local and regional administration. 

People's committees were established in such widely divergent organizations as universities, private business firms, government bureaucracies, and the broadcast media. Geographically based committees were formed at the governorate, municipal, and zone (lowest) levels. Seats on the people's committees at the zone level were filled by direct popular election; members so elected could then be selected for service at higher levels. By mid-1973 estimates of the number of people's committees ranged above 2,000. In the scope of their administrative and regulatory tasks and the method of their members' selection, the people's committees purportedly embodied the concept of direct democracy that Gaddafi propounded in the first volume of The Green Book, which appeared in 1976. The same concept lay behind proposals to create a new political structure composed of "people's congresses." The centerpiece of the new system was the General People's Congress (GPC), a national representative body intended to replace the RCC.

Libyan–Egyptian War

On July 21, 1977, there were first gun battles between troops on the border, followed by land and air strikes. Relations between the Libyan and the Egyptian government had been deteriorating ever since the end of Yom Kippur War from October 1973, due to Libyan opposition to President Anwar Sadat's peace policy as well as the breakdown of unification talks between the two governments. There is some proof that the Egyptian government was considering a war against Libya as early as 1974. On February 28, 1974, during Henry Kissinger's visit to Egypt, President Sadat told him about such intentions and requested that pressure be put on the Israeli government not to launch an attack on Egypt in the event of its forces being occupied in war with Libya. In addition, the Egyptian government had broken its military ties with Moscow, while the Libyan government kept that cooperation going. The Egyptian government also gave assistance to former RCC members Major Abd al Munim al Huni and Omar Muhayshi, who unsuccessfully tried to overthrow Muammar Gaddafi in 1975, and allowed them to reside in Egypt. During 1976 relations were ebbing, as the Egyptian government claimed to have discovered a Libyan plot to overthrow the government in Cairo. On January 26, 1976, Egyptian Vice President Hosni Mubarak indicated in a talk with the US Ambassador Hermann Eilts that the Egyptian government intended to exploit internal problems in Libya to promote actions against Libya, but did not elaborate. On July 22, 1976, the Libyan government made a public threat to break diplomatic relations with Cairo if Egyptian subversive actions continued. On August 8, 1976, an explosion occurred in the bathroom of a government office in Tahrir Square in Cairo, injuring 14, and the Egyptian government and media claimed this was done by Libyan agents. The Egyptian government also claimed to have arrested two Egyptian citizens trained by Libyan intelligence to perform sabotage within Egypt. On August 23, an Egyptian passenger plane was hijacked by persons who reportedly worked with Libyan intelligence. They were captured by Egyptian authorities in an operation that ended without any casualties. In retaliation for accusations by the Egyptian government of Libyan complicity in the hijacking, the Libyan government ordered the closure of the Egyptian Consulate in Benghazi. On July 24, the combatants agreed to a ceasefire under the mediation of the President of Algeria Houari Boumediène and the Palestine Liberation Organization leader Yasser Arafat.

Great Socialist People's Libyan Arab Jamahiriya (1977–2011)

Socialist People's Libyan Arab Jamahiriya (1977–1986)
Great Socialist People's Libyan Arab Jamahiriya (1986–2011)

الجماهيرية العربية الليبية الشعبية الإشتراكية العظمى
al-Jamāhīrīyah al-'Arabīyah al-Lībīyah ash-Sha'bīyah al-Ishtirākīyah al-'Uẓmá
1977–2011
Anthem: الله أكبر
"Allahu Akbar"
(English: "God is Great")
Location of Libya
CapitalTripoli (1977–2011)
Sirte (2011)
32°52′N 13°11′E
Largest cityTripoli
Official languagesArabic
Spoken languages
Minority Languages
Ethnic groups
Religion Islam
GovernmentUnitary Islamic socialist Jamahiriya under a totalitarian dictatorship
Brotherly Leader and Guide of the Revolution 

• 1977–2011
Muammar Gaddafi
Historical eraCold War · War on Terror

• People's Authority
2 March 1977
28 August 2011
20 October 2011
Area
• Total
1,759,541 km2 (679,363 sq mi) (16th)
Population

• 2010
6,355,100
HDI (2009)Increase 0.847
very high
CurrencyLibyan dinar
Calling code218
ISO 3166 codeLY
Preceded by Succeeded by
Libyan Arab Republic
National Transitional Council

On 2 March 1977, the General People's Congress (GPC), at Gaddafi's behest, adopted the "Declaration of the Establishment of the People's Authority" and proclaimed the Socialist People's Libyan Arab Jamahiriya (Arabic: الجماهيرية العربية الليبية الشعبية الاشتراكيةal-Jamāhīrīyah al-'Arabīyah al-Lībīyah ash-Sha'bīyah al-Ishtirākīyah). In the official political philosophy of Gaddafi's state, the "Jamahiriya" system was unique to the country, although it was presented as the materialization of the Third International Theory, proposed by Gaddafi to be applied to the entire Third World. The GPC also created the General Secretariat of the GPC, comprising the remaining members of the defunct Revolutionary Command Council, with Gaddafi as general secretary, and also appointed the General People's Committee, which replaced the Council of Ministers, its members now called secretaries rather than ministers.

The Libyan government claimed that the Jamahiriya was a direct democracy without any political parties, governed by its populace through local popular councils and communes (named Basic People's Congresses). Official rhetoric disdained the idea of a nation state, tribal bonds remaining primary, even within the ranks of the Armed Forces of the Libyan Arab Jamahiriya.

Etymology

Jamahiriya (Arabic: جماهيريةjamāhīrīyah) is an Arabic term generally translated as "state of the masses"; Lisa Anderson  has suggested "peopledom" or "state of the masses" as a reasonable approximations of the meaning of the term as intended by Gaddafi. The term does not occur in this sense in Muammar Gaddafi's Green Book of 1975. The nisba-adjective jamāhīrīyah ("mass-, "of the masses") occurs only in the third part, published in 1981, in the phrase إن الحركات التاريخية هي الحركات الجماهيرية (Inna al-ḥarakāt at-tārīkhīyah hiya al-ḥarakāt al-jamāhīrīyah), translated in the English edition as "Historic movements are mass movements".

The word jamāhīrīyah was derived from jumhūrīyah, which is the usual Arabic translation of "republic". It was coined by changing the component jumhūr — "public" — to its plural form, jamāhīr — "the masses". Thus, it is similar to the term People's Republic. It is often left untranslated in English, with the long-form name thus rendered as Great Socialist People's Libyan Arab Jamahiriya. However, in Hebrew, for instance, jamāhīrīyah is translated as "קהילייה" (qehiliyáh), a word also used to translate the term "Commonwealth" when referring to the designation of a country.
After weathering the 1986 U.S. bombing by the Reagan administration, Gaddafi added the specifier "Great" (العظمى al-'Uẓmá) to the official name of the country.

Reforms (1977–1980)

The Economist Intelligence Unit's Democracy Index 2010
Full democracies:
  9–10
  8–8.9
Flawed democracies:
  7–7.9
  6–6.9
  No data
Hybrid regimes:
  5–5.9
  4–4.9
Authoritarian regimes:
  3–3.9
  2–2.9
  0–1.9

The UNDP's Human Development Index (based on 2010 data, published on 4 November 2010)
  0.900 and over
  0.850–0.899
  0.800–0.849
  0.750–0.799
  0.700–0.749
  0.650–0.699
  0.600–0.649
  0.550–0.599
  0.500–0.549
  0.450–0.499
  0.400–0.449
  0.350–0.399
  0.300–0.349
  under 0.300
  Data unavailable

Gaddafi as permanent "Leader of the Revolution"

The changes in Libyan leadership since 1976 culminated in March 1979, when the General People's Congress declared that the "vesting of power in the masses" and the "separation of the state from the revolution" were complete. The government was divided into two parts, the "Jamahiriya sector" and the "revolutionary sector". The "Jamahiriya sector" was composed of the General People's Congress, the General People's Committee, and the local Basic People's Congresses. Gaddafi relinquished his position as general secretary of the General People's Congress, as which he was succeeded by Abdul Ati al-Obeidi, who had been prime minister since 1977.

The "Jamahiriya sector" was overseen by the "revolutionary sector", headed by Gaddafi as "Leader of the Revolution" (Qā'id) and the surviving members of the Revolutionary Command Council, which held office owing to their role in the 1969 coup and were therefore not subject to election. They oversaw the "revolutionary committees", which were nominally grass-roots organizations that helped keep the people engaged. As a result, although Gaddafi held no formal government office after 1979, he retained control of the government and the country. Gaddafi also remained supreme commander of the armed forces.

Administrative reforms

All legislative and executive authority was vested in the GPC. This body, however, delegated most of its important authority to its general secretary and General Secretariat and to the General People's Committee. Gaddafi, as general secretary of the GPC, remained the primary decision maker, just as he had been when chairman of the RCC. In turn, all adults had the right and duty to participate in the deliberation of their local Basic People's Congress (BPC), whose decisions were passed up to the GPC for consideration and implementation as national policy. The BPCs were in theory the repository of ultimate political authority and decision making, embodying what Gaddafi termed direct "people's power". The 1977 declaration and its accompanying resolutions amounted to a fundamental revision of the 1969 constitutional proclamation, especially with respect to the structure and organization of the government at both national and subnational levels. 

Continuing to revamp Libya's political and administrative structure, Gaddafi introduced yet another element into the body politic. Beginning in 1977, "revolutionary committees" were organized and assigned the task of "absolute revolutionary supervision of people's power"; that is, they were to guide the people's committees, "raise the general level of political consciousness and devotion to revolutionary ideals". In reality, the revolutionary committees were used to survey the population and repress any political opposition to Gaddafi's autocratic rule. Reportedly 10% to 20% of Libyans worked in surveillance for these committees, a proportion of informants on par with Ba'athist Iraq or Juche Korea.

Filled with politically astute zealots, the ubiquitous revolutionary committees in 1979 assumed control of BPC elections. Although they were not official government organs, the revolutionary committees became another mainstay of the domestic political scene. As with the people's committees and other administrative innovations since the revolution, the revolutionary committees fit the pattern of imposing a new element on the existing subnational system of government rather than eliminating or consolidating already existing structures. By the late 1970s, the result was an unnecessarily complex system of overlapping jurisdictions in which cooperation and coordination among different elements were compromised by ill-defined authority and responsibility. The ambiguity may have helped serve Gaddafi's aim to remain the prime mover behind Libyan governance, while minimizing his visibility at a time when internal opposition to political repression was rising. 

The RCC was formally dissolved and the government was again reorganized into people's committees. A new General People's Committee (cabinet) was selected, each of its "secretaries" becoming head of a specialized people's committee; the exceptions were the "secretariats" of petroleum, foreign affairs, and heavy industry, where there were no people's committees. A proposal was also made to establish a "people's army" by substituting a national militia, being formed in the late 1970s, for the national army. Although the idea surfaced again in early 1982, it did not appear to be close to implementation. 

Gaddafi also wanted to combat the strict social restrictions that had been imposed on women by the previous regime, establishing the Revolutionary Women's Formation to encourage reform. In 1970, a law was introduced affirming equality of the sexes and insisting on wage parity. In 1971, Gaddafi sponsored the creation of a Libyan General Women's Federation. In 1972, a law was passed criminalizing the marriage of any females under the age of sixteen and ensuring that a woman's consent was a necessary prerequisite for a marriage.

Economic reforms

Libya under Gaddafi used to have a higher GDP (PPP) per capita than the EU, and in some periods higher than the US.
 
Remaking of the economy was parallel with the attempt to remold political and social institutions. Until the late 1970s, Libya's economy was mixed, with a large role for private enterprise except in the fields of oil production and distribution, banking, and insurance. But according to volume two of Gaddafi's Green Book, which appeared in 1978, private retail trade, rent, and wages were forms of exploitation that should be abolished. Instead, workers' self-management committees and profit participation partnerships were to function in public and private enterprises.

A property law was passed that forbade ownership of more than one private dwelling, and Libyan workers took control of a large number of companies, turning them into state-run enterprises. Retail and wholesale trading operations were replaced by state-owned "people's supermarkets", where Libyans in theory could purchase whatever they needed at low prices. By 1981 the state had also restricted access to individual bank accounts to draw upon privately held funds for government projects. The measures created resentment and opposition among the newly dispossessed. The latter joined those already alienated, some of whom had begun to leave the country. By 1982, perhaps 50,000 to 100,000 Libyans had gone abroad; because many of the emigrants were among the enterprising and better educated Libyans, they represented a significant loss of managerial and technical expertise.

The government also built a trans-Sahara water pipeline from major aquifers to both a network of reservoirs and the towns of Tripoli, Sirte and Benghazi in 2006–2007. It is part of the Great Manmade River project, started in 1984. It is pumping large resources of water from the Nubian Sandstone Aquifer System to both urban populations and new irrigation projects around the country.

Libya continued to be plagued with a shortage of skilled labor, which had to be imported along with a broad range of consumer goods, both paid for with petroleum income. The country consistently ranked as the African nation with the highest HDI, standing at 0.755 in 2010, which was 0.041 higher than the next highest African HDI that same year. Gender equality was a major achievement under Gaddafi's rule. According to Lisa Anderson, president of the American University in Cairo and an expert on Libya, said that under Gaddafi more women attended university and had "dramatically" more employment opportunities.

Military

Wars against Chad and Egypt

As early as 1969, Gaddafi waged a campaign against Chad. Scholar Gerard Prunier claims part of his hostility was apparently because Chadian President François Tombalbaye was Christian. Libya was also involved in a sometimes violent territorial dispute with neighbouring Chad over the Aouzou Strip, which Libya occupied in 1973. This dispute eventually led to the Libyan invasion of Chad. The prolonged foray of Libyan troops into the Aozou Strip in northern Chad, was finally repulsed in 1987, when extensive US and French help to Chadian rebel forces and the government headed by former Defence Minister Hissein Habré finally led to a Chadian victory in the so-called Toyota War. The conflict ended in a ceasefire in 1987. After a judgement of the International Court of Justice on 13 February 1994, Libya withdrew troops from Chad the same year and the dispute was settled.

In 1977, Gaddafi dispatched his military across the border to Egypt, but Egyptian forces fought back in the Libyan–Egyptian War. Both nations agreed to a ceasefire under the mediation of the President of Algeria Houari Boumediène.

Islamic Legion

In 1972, Gaddafi created the Islamic Legion as a tool to unify and Arabize the region. The priority of the Legion was first Chad, and then Sudan. In Darfur, a western province of Sudan, Gaddafi supported the creation of the Arab Gathering (Tajammu al-Arabi), which according to Gérard Prunier was "a militantly racist and pan-Arabist organization which stressed the 'Arab' character of the province." The two organizations shared members and a source of support, and the distinction between them is often ambiguous.

This Islamic Legion was mostly composed of immigrants from poorer Sahelian countries, but also, according to a source, thousands of Pakistanis who had been recruited in 1981 with the false promise of civilian jobs once in Libya. Generally speaking, the Legion's members were immigrants who had gone to Libya with no thought of fighting wars, and had been provided with inadequate military training and had sparse commitment. A French journalist, speaking of the Legion's forces in Chad, observed that they were "foreigners, Arabs or Africans, mercenaries in spite of themselves, wretches who had come to Libya hoping for a civilian job, but found themselves signed up more or less by force to go and fight in an unknown desert."

At the beginning of the 1987 Libyan offensive in Chad, it maintained a force of 2,000 in Darfur. The nearly continuous cross-border raids that resulted greatly contributed to a separate ethnic conflict within Darfur that killed about 9,000 people between 1985 and 1988.

Janjaweed, a group accused by the US of carrying out a genocide in Darfur in the 2000s, emerged in 1988 and some of its leaders are former legionnaires.

Attempts at nuclear and chemical weapons

In 1972, Gaddafi tried to buy a nuclear bomb from the People's Republic of China. He then tried to get a bomb from Pakistan, but Pakistan severed its ties before it succeeded in building a bomb. In 1978, Gaddafi turned to Pakistan's rival, India, for help building its own nuclear bomb. In July 1978, Libya and India signed a memorandum of understanding to cooperate in peaceful applications of nuclear energy as part of India's Atom of Peace policy. In 1991, then Prime Minister Navaz Sharif paid a state visit to Libya to hold talks on the promotion of a Free Trade Agreement between Pakistan and Libya. However, Gaddafi focused on demanding Pakistan's Prime Minister sell him a nuclear weapon, which surprised many of the Prime Minister's delegation members and journalists. When Prime minister Sharif refused Gaddafi's demand, Gaddafi disrespected him, calling him a "Corrupt politician", a term which insulted and surprised Sharif. The Prime minister cancelled the talks, returned to Pakistan and expelled the Libyan Ambassador from Pakistan.

Thailand reported its citizens had helped build storage facilities for nerve gas. Germany sentenced a businessman, Jurgen Hippenstiel-Imhausen, to five years in prison for involvement in Libyan chemical weapons. Inspectors from the Chemical Weapons Convention (CWC) verified in 2004 that Libya owned a stockpile of 23 metric tons of mustard gas and more than 1,300 metric tons of precursor chemicals.

Gulf of Sidra incidents and US air strikes

When Libya was under pressure from international disputes, on 19 August 1981, a naval dogfight occurred over the Gulf of Sirte in the Mediterranean Sea. US F-14 Tomcat jets fired anti-aircraft missiles against a formation of Libyan fighter jets in this dogfight and shot down two Libyan Su-22 Fitter attack aircraft. This naval action was a result of claiming the territory and losses from the previous incident. A second dogfight occurred on 4 January 1989; US carrier-based jets also shot down two Libyan MiG-23 Flogger-Es in the same place. 

A similar action occurred on 23 March 1986; while patrolling the Gulf, US naval forces attacked a sizable naval force and various SAM sites defending Libyan territory. US fighter jets and fighter-bombers destroyed SAM launching facilities and sank various naval vessels, killing 35 seamen. This was a reprisal for terrorist hijackings between June and December 1985.

On 5 April 1986, Libyan agents bombed "La Belle" nightclub in West Berlin, killing three and injuring 229. Gaddafi's plan was intercepted by several national intelligence agencies and more detailed information was retrieved four years later from Stasi archives. The Libyan agents who had carried out the operation, from the Libyan embassy in East Germany, were prosecuted by the reunited Germany in the 1990s.

In response to the discotheque bombing, joint US Air Force, Navy and Marine Corps air-strikes took place against Libya on 15 April 1986 and code-named Operation El Dorado Canyon and known as the 1986 bombing of Libya. Air defenses, three army bases, and two airfields in Tripoli and Benghazi were bombed. The surgical strikes failed to kill Gaddafi but he lost a few dozen military officers. Gaddafi spread propaganda how it had killed his "adopted daughter" and how victims had been all "civilians". Despite the variations of the stories, the campaign was successful, and a large proportion of the Western press reported the government's stories as facts.

Following the 1986 bombing of Libya, Gaddafi intensified his support for anti-American government organizations. He financed Jeff Fort's Al-Rukn faction of the Chicago Black P. Stones gang, in their emergence as an indigenous anti-American armed revolutionary movement. Al-Rukn members were arrested in 1986 for preparing strikes on behalf of Libya, including blowing up US government buildings and bringing down an airplane; the Al-Rukn defendants were convicted in 1987 of "offering to commit bombings and assassinations on US soil for Libyan payment." In 1986, Libyan state television announced that Libya was training suicide squads to attack American and European interests. He began financing the IRA again in 1986, to retaliate against the British for harboring American fighter planes.

Gaddafi announced that he had won a spectacular military victory over the US and the country was officially renamed the "Great Socialist People's Libyan Arab Jamahiriyah". However, his speech appeared devoid of passion and even the "victory" celebrations appeared unusual. Criticism of Gaddafi by ordinary Libyan citizens became more bold, such as defacing of Gaddafi posters. The raids against Libyan military had brought the government to its weakest point in 17 years.

International relations

Africa

Gaddafi was a close supporter of Ugandan President Idi Amin.

Gaddafi sent thousands of troops to fight against Tanzania on behalf of Idi Amin. About 600 Libyan soldiers lost their lives attempting to defend the collapsing presidency of Amin. Amin was eventually exiled from Uganda to Libya before settling in Saudi Arabia.

Gaddafi also aided Jean-Bédel Bokassa, the Emperor of the Central African Empire. He also intervened militarily in the renewed Central African Republic in 2001 to protect his ally Ange-Félix Patassé. Patassé signed a deal giving Libya a 99-year lease to exploit all of that country's natural resources, including uranium, copper, diamonds, and oil.

Gaddafi supported Soviet protégé Haile Mariam Mengistu.

Gaddafi was a strong opponent of apartheid in South Africa and forged a friendship with Nelson Mandela. One of Mandala's grandsons is named Gaddafi, an indication of the latter's support in South Africa. Gaddafi funded Mandela's 1994 election campaign, and after taking office as the country's first democratically-elected president in 1994, Mandela rejected entreaties from U.S. President Bill Clinton and others to cut ties with Gaddafi. Mandela later played a key role in helping Gaddafi gain mainstream acceptance in the Western world later in the 1990s. Over the years, Gaddafi came to be seen as a hero in much of Africa due to his revolutionary image.

Gaddafi was a strong supporter of Zimbabwean President Robert Mugabe.

Gaddafi's World Revolutionary Center (WRC) near Benghazi became a training center for groups backed by Gaddafi. Graduates in power as of 2011 include Blaise Compaoré of Burkina Faso and Idriss Déby of Chad.

Gaddafi trained and supported Liberian warlord-president Charles Taylor, who was indicted by the Special Court for Sierra Leone for war crimes and crimes against humanity committed during the conflict in Sierra Leone. Foday Sankoh, the founder of Revolutionary United Front, was also Gaddafi's graduate. According to Douglas Farah, "The amputation of the arms and legs of men, women, and children as part of a scorched-earth campaign was designed to take over the region's rich diamond fields and was backed by Gaddafi, who routinely reviewed their progress and supplied weapons".

Gaddafi's strong military support and finances gained him allies across the continent. He had himself crowned with the title "King of Kings of Africa" in 2008, in the presence of over 200 African traditional rulers and kings, although his views on African political and military unification received a lukewarm response from their governments. His 2009 forum for African kings was canceled by the Ugandan hosts, who believed that traditional rulers discussing politics would lead to instability. On 1 February 2009, a 'coronation ceremony' in Addis Ababa, Ethiopia, was held to coincide with the 53rd African Union Summit, at which he was elected head of the African Union for the year. Gaddafi told the assembled African leaders: "I shall continue to insist that our sovereign countries work to achieve the United States of Africa."

In 1986, 2000, and the months prior to the 2011 civil war, Gaddafi announced plans for a unified African gold dinar currency, to challenge the dominance of the US Dollar and Euro currencies. The African dinar would have been measured directly in terms of gold.

Gaddafi and international terrorism

In 1971 Gaddafi warned that if France opposes Libyan military occupation of Chad, he will use all weapons in the war against France including the "revolutionary weapon". On 11 June 1972, Gaddafi announced that any Arab wishing to volunteer for Palestinian terrorist groups "can register his name at any Libyan embassy will be given adequate training for combat". He also promised financial support for attacks. On 7 October 1972, Gaddafi praised the Lod Airport massacre, executed by the communist Japanese Red Army, and demanded Palestinian terrorist groups to carry out similar attacks.

Reportedly, Gaddafi was a major financier of the "Black September Movement" which perpetrated the Munich massacre at the 1972 Summer Olympics. In 1973 the Irish Naval Service intercepted the vessel Claudia in Irish territorial waters, which carried Soviet arms from Libya to the Provisional IRA. In 1976 after a series of terror activities by the Provisional IRA, Gaddafi announced that "the bombs which are convulsing Britain and breaking its spirit are the bombs of Libyan people. We have sent them to the Irish revolutionaries so that the British will pay the price for their past deeds".

In the Philippines, Libya backed the Moro Islamic Liberation Front, which continues to carry out acts of violence in an effort to establish a separatist Islamic state in the southern Philippines. Libya has also supported the New People's Army and Libyan agents were seen meeting with the Communist Party of the Philippines. Islamist terrorist group Abu Sayyaf has also been suspected of receiving Libyan funding.

Gaddafi also became a strong supporter of the Palestine Liberation Organization, which support ultimately harmed Libya's relations with Egypt, when in 1979 Egypt pursued a peace agreement with Israel. As Libya's relations with Egypt worsened, Gaddafi sought closer relations with the Soviet Union. Libya became the first country outside the Soviet bloc to receive the supersonic MiG-25 combat fighters, but Soviet-Libyan relations remained relatively distant. Gaddafi also sought to increase Libyan influence, especially in states with an Islamic population, by calling for the creation of a Saharan Islamic state and supporting anti-government forces in sub-Saharan Africa

In the 1970s and the 1980s, this support was sometimes so freely given that even the most unsympathetic groups could obtain Libyan support; often the groups represented ideologies far removed from Gaddafi's own. Gaddafi's approach often tended to confuse international opinion. 

In October 1981 Egypt's President Anwar Sadat was assassinated. Gaddafi applauded the murder and remarked that it was a "punishment".

In December 1981, the US State Department invalidated US passports for travel to Libya, and in March 1982, the U.S. declared a ban on the import of Libyan oil.

Gaddafi reportedly spent hundreds of millions of the government's money on training and arming Sandinistas in Nicaragua. Daniel Ortega, the President of Nicaragua, was his ally. 

In April 1984, Libyan refugees in London protested against execution of two dissidents. Communications intercepted by MI5 show that Tripoli ordered its diplomats to direct violence against the demonstrators. Libyan diplomats shot at 11 people and killed British policewoman Yvonne Fletcher. The incident led to the breaking off of diplomatic relations between the United Kingdom and Libya for over a decade.

After December 1985 Rome and Vienna airport attacks, which killed 19 and wounded around 140, Gaddafi indicated that he would continue to support the Red Army Faction, the Red Brigades, and the Irish Republican Army as long as European countries support anti-Gaddafi Libyans. The Foreign Minister of Libya also called the massacres "heroic acts".

In 1986, Libyan state television announced that Libya was training suicide squads to attack American and European interests.

On 5 April 1986, Libyan agents were alleged with bombing the "La Belle" nightclub in West Berlin, killing three people and injuring 229 people who were spending evening there. Gaddafi's plan was intercepted by Western intelligence. More-detailed information was retrieved years later when Stasi archives were investigated by the reunited Germany. Libyan agents who had carried out the operation from the Libyan embassy in East Germany were prosecuted by reunited Germany in the 1990s.

In May 1987, Australia broke off relations with Libya because of its role in fueling violence in Oceania.

Under Gaddafi, Libya had a long history of supporting the Irish Republican Army. In late 1987 French authorities stopped a merchant vessel, the MV Eksund, which was delivering a 150-ton Libyan arms shipment to the IRA. In Britain, Gaddafi's best-known political subsidiary is the Workers Revolutionary Party.

Gaddafi fueled a number of Islamist and communist groups in the Philippines, including the New People's Army of the Communist Party of the Philippines and the Moro Islamic Liberation Front.

In Indonesia, the Free Aceh Movement was a Libyan-backed militant group. Vanuatu's ruling party enjoyed Libyan support.

In New Zealand, Libya attempted to radicalize Māoris.

In Australia, there were several cases of attempted radicalisation of Australian Aborigines, with individuals receiving paramilitary training in Libya. Libya put several left-wing unions on the Libyan payroll, such as the Food Preservers Union (FPU) and the Federated Confectioners Association of Australia (FCA). Labour Party politician Bill Hartley, the secretary of Libya-Australia friendship society, was long-term supporter of Gaddafi and Saddam Hussein.

In the 1980s, the Libyan government purchased advertisements in Arabic-language newspapers in Australia asking for Australian Arabs to join the military units of his worldwide struggle against imperialism. In part, because of this, Australia banned recruitment of foreign mercenaries in Australia.

Gaddafi developed a relationship with the Revolutionary Armed Forces of Colombia, becoming acquainted with its leaders in meetings of revolutionary groups regularly hosted in Libya.

Some publications were financed by Gaddafi. The Socialist Labour League's Workers News was one such publication: "in among the routine denunciations of uranium mining and calls for greater trade union militancy would be a couple of pages extolling Gaddafi's fatuous and incoherent green book and the Libyan revolution."

International sanctions after the Lockerbie bombing (1992–2003)

Libya was accused in the 1988 bombing of Pan Am Flight 103 over Lockerbie, Scotland; UN sanctions were imposed in 1992. UN Security Council resolutions (UNSCRs) passed in 1992 and 1993 obliged Libya to fulfill requirements related to the Pan Am 103 bombing before sanctions could be lifted, leading to Libya's political and economic isolation for most of the 1990s. The UN sanctions cut airline connections with the outer world, reduced diplomatic representation and prohibited the sale of military equipment. Oil-related sanctions were assessed by some as equally significant for their exceptions: thus sanctions froze Libya's foreign assets (but excluded revenue from oil and natural gas and agricultural commodities) and banned the sale to Libya of refinery or pipeline equipment (but excluded oil production equipment).

Under the sanctions Libya's refining capacity eroded. Libya's role on the international stage grew less provocative after UN sanctions were imposed. In 1999, Libya fulfilled one of the UNSCR requirements by surrendering two Libyans suspected in connection with the bombing for trial before a Scottish court in the Netherlands. One of these suspects, Abdel Basset al-Megrahi, was found guilty; the other was acquitted. UN sanctions against Libya were subsequently suspended. The full lifting of the sanctions, contingent on Libya's compliance with the remaining UNSCRs, including acceptance of responsibility for the actions of its officials and payment of appropriate compensation, was passed 12 September 2003, explicitly linked to the release of up to $2.7 billion in Libyan funds to the families of the 1988 attack's 270 victims.

In 2002, Gaddafi paid a ransom reportedly worth tens of millions of dollars to Abu Sayyaf, a Filipino Islamist militancy, to release a number of kidnapped tourists. He presented it as an act of goodwill to Western countries; nevertheless the money helped the group to expand its operation.

Normalization of international relations (2003–2010)

In December 2003, Libya announced that it had agreed to reveal and end its programs to develop weapons of mass destruction and to renounce terrorism, and Gaddafi made significant strides in normalizing relations with western nations. He received various Western European leaders as well as many working-level and commercial delegations, and made his first trip to Western Europe in 15 years when he traveled to Brussels in April 2004. Libya responded in good faith to legal cases brought against it in U.S. courts for terrorist acts that predate its renunciation of violence. Claims for compensation in the Lockerbie bombing, LaBelle disco bombing, and UTA 772 bombing cases are ongoing. The U.S. rescinded Libya's designation as a state sponsor of terrorism in June 2006. In late 2007, Libya was elected by the General Assembly to a nonpermanent seat on the United Nations Security Council for the 2008–2009 term. Currently, Operation Enduring Freedom - Trans Sahara is being fought in Libya's portion of the Sahara Desert.

Purification laws

In 1994, the General People's Congress approved the introduction of "purification laws" to be put into effect, punishing theft by the amputation of limbs, and fornication and adultery by flogging. Under the Libyan constitution, homosexual relations are punishable by up to five years in jail.

Opposition, coups and revolts

Throughout his long rule, Gaddafi had to defend his position against opposition and coup attempts, emerging both from the military and from the general population. He reacted to these threats on one hand by maintaining a careful balance of power between the forces in the country, and by brutal repression on the other. Gaddafi successfully balanced the various tribes of Libya one against the other by distributing his favours. To forestall a military coup, he deliberately weakened the Libyan Armed Forces by regularly rotating officers, relying instead on loyal elite troops such as his Revolutionary Guard Corps, the special-forces Khamis Brigade and his personal Amazonian Guard, even though emphasis on political loyalty tended, over the long run, to weaken the professionalism of his personal forces. This trend made the country vulnerable to dissension at a time of crisis, as happened during early 2011.

Political repression and "Green Terror"

The term "Green Terror" is used to describe campaigns of violence and intimidation against opponents of Gaddafi, particularly in reference to wave of oppression during Libya's cultural revolution, or to the wave of highly publicized hangings of regime opponents that began with the Execution of Al-Sadek Hamed Al-Shuwehdy. Dissent was illegal under Law 75 of 1973. Reportedly 10 to 20 percent of Libyans worked in surveillance for Gaddafi's Revolutionary Committees, a proportion of informants on par with Saddam Hussein's Iraq or Kim Jong Il's North Korea. The surveillance took place in government, in factories, and in the education sector.

Following an abortive attempt to replace English foreign language education with Russian, in recent years English has been taught in Libyan schools from a primary level, and students have access to English-language media. However, one protester in 2011 described the situation as: "None of us can speak English or French. He kept us ignorant and blindfolded".

According to the 2009 Freedom of the Press Index, Libya is the most censored country in the Middle East and North Africa. Prisons are run with little or no documentation of the inmate population or of such basic data as prisoner's crime and sentence.

Opposition to the Jamahiriya reforms

During the late 1970s, some exiled Libyans formed active opposition groups. In early 1979, Gaddafi warned opposition leaders to return home immediately or face "liquidation." When caught, they could face being sentenced and hanged in public.
It is the Libyan people's responsibility to liquidate such scums who are distorting Libya's image abroad.
— Gaddafi talking about exiles in 1982.
Gaddafi employed his network of diplomats and recruits to assassinate dozens of his critics around the world. Amnesty International listed at least twenty-five assassinations between 1980 and 1987.

Gaddafi's agents were active in the U.K., where many Libyans had sought asylum. After Libyan diplomats shot at 15 anti-Gaddafi protesters from inside the Libyan embassy's first floor and killed a British policewoman, the U.K. broke off relations with Gaddafi's government as a result of the incident.

Even the U.S. could not protect dissidents from Libya. In 1980, a Libyan agent attempted to assassinate dissident Faisal Zagallai, a doctoral student at the University of Colorado at Boulder. The bullets left Zagallai partially blinded. A defector was kidnapped and executed in 1990 just before he was about to receive U.S. citizenship.

Gaddafi asserted in June 1984 that killings could be carried out even when the dissidents were on pilgrimage in the holy city of Mecca. In August 1984, one Libyan plot was thwarted in Mecca.[57]
As of 2004, Libya still provided bounties for heads of critics, including 1 million dollars for Ashur Shamis, a Libyan-British journalist.

There is indication that between the years of 2002 and 2007, Libya's Gaddafi-era intelligence service had a partnership with western spy organizations including MI6 and the CIA, who voluntarily provided information on Libyan dissidents in the United States and Canada in exchange for using Libya as a base for extraordinary renditions. This was done despite Libya's history of murdering dissidents abroad, and with full knowledge of Libya's brutal mistreatment of detainees.

Political unrest during the 1990s

In the 1990s, Gaddafi's rule was threatened by militant Islamism. In October 1993, there was an unsuccessful assassination attempt on Gaddafi by elements of the Libyan army. In response, Gaddafi used repressive measures, using his personal Revolutionary Guard Corps to crush riots and Islamist activism during the 1990s. Nevertheless, Cyrenaica between 1995 and 1998 was politically unstable, due to the tribal allegiances of the local troops.

2011 civil war and collapse of Gaddafi's government

A global map of the world showing countries that recognised or had informal relations with the Libyan Republic during the civil war of 2011.
  Libya
  Countries that recognised the National Transitional Council (NTC) as the sole legitimate representative of Libya
  Countries that had permanent informal relations with the NTC, or which voted in favor of recognition at the UN, but had not granted official recognition
  Countries which opposed recognition of the NTC at the UN, but had not made a formal statement
  Countries that said they would not recognise the NTC

A renewed serious threat to the Libyan Arab Jamahiriya came in February 2011, with the Libyan Civil War. The novelist Idris Al-Mesmari was arrested hours after giving an interview with Al Jazeera about the police reaction to protests in Benghazi on 15 February. 

Inspiration for the unrest is attributed to the uprisings in Tunisia and Egypt, connecting it with the wider Arab Spring. On 22 February, The Economist described the events as an "uprising that is trying to reclaim Libya from the world's longest-ruling autocrat." Gaddafi had referred to the opposition variously as "rats", "cockroaches", and "drugged kids" and accused them of being part of al-Qaeda. In the east, the National Transitional Council was established in Benghazi.

Gaddafi's son, Khamis, controlled the well-armed Khamis Brigade and alleged to possess large number of mercenaries. Some Libyan officials had sided with the protesters and requested help from the international community to bring an end to the massacres of civilians. The government in Tripoli had lost control of half of Libya by the end of February, but as of mid-September Gaddafi remained in control of several parts of Fezzan. On 21 September, the forces of NTC captured Sabha, the largest city of Fezzan, reducing the control of Gaddafi to limited and isolated areas. 

Many nations condemned Gaddafi's government over its use of force against civilians. Several other nations allied with Gaddafi called the uprising and intervention a "plot" by Western powers to loot Libya's resources. The United Nations Security Council passed a resolution to enforce a no-fly zone over Libyan airspace on 17 March 2011.

The UN resolution authorised air-strikes against Libyan ground troops and warships that appeared to threaten civilians. On 19 March, the no-fly zone enforcement began, with French aircraft undertaking sorties across Libya and a naval blockade by the British Royal Navy. Eventually, the aircraft carriers USS Enterprise and Charles de Gaulle arrived off the coast and provided the enforcers with a rapid-response capability. U.S. forces named their part of the enforcement action Operation Odyssey Dawn, meant to "deny the Libyan regime from using force against its own people". said U.S. Vice Admiral William E. Gortney. More than 110 "Tomahawk" cruise missiles were fired in an initial assault by U.S. warships and a British submarine against Libyan air defences.

The last government holdouts in Sirte finally fell to anti-Gaddafi fighters on 20 October 2011, and, following the controversial death of Muammar Gaddafi, Libya was officially declared "liberated" on 23 October 2011, ending 42 years of Gaddafi's leadership in Libya.

Political scientist Riadh Sidaoui suggested in October 2011 that Gaddafi "has created a great void for his exercise of power: there is no institution, no army, no electoral tradition in the country", and as a result, the period of transition would be difficult in Libya.

Molecularly imprinted polymer

From Wikipedia, the free encyclopedia
 
A molecularly imprinted polymer (MIP) is a polymer that has been processed using the molecular imprinting technique which leaves cavities in the polymer matrix with an affinity for a chosen "template" molecule. The process usually involves initiating the polymerization of monomers in the presence of a template molecule that is extracted afterwards, leaving behind complementary cavities. These polymers have affinity for the original molecule and have been used in applications such as chemical separations, catalysis, or molecular sensors. Published works on the topic date to the 1930s.

Molecular imprinting techniques (state of the art and perspectives)

Molecular imprinting is the process of generating an impression within a solid or a gel, the size, shape and charge distribution of which corresponds to a template molecule (typically present during polymerisation). The result is a synthetic receptor capable of binding to a target molecule, which fits into the binding site with high affinity and specificity. The interactions between the polymer and the template are similar to those between antibodies and antigens, consisting of electrostatic interactions, hydrogen bonds, Van der Waals forces, and hydrophobic interactions

One of the greatest advantages of artificial receptors over naturally occurring receptors is freedom of molecular design. Their frameworks are not restricted to proteins, and a variety of skeletons (e.g., carbon chains and fused aromatic rings) can be used. Thus, the stability, flexibility, and other properties are freely modulated according to need. Even functional groups that are not found in nature can be employed in these synthetic compounds. Furthermore, when necessary, the activity in response towards outer stimuli (photo-irradiation, pH change, electric or magnetic field, and others) can be provided by using appropriate functional groups.

In a molecular imprinting processes, one needs a 1) template, 2) functional monomer(s) 3) cross-linker, 4) radical or other polymerization initiator, 5) porogenic solvent and 6) extraction solvent. According to polymerization method and final polymer format one or some of the reagent can be avoided.

Preparation of molecularly imprinted material

There are two main methods for creating these specialized polymers. The first is known as self-assembly, which involves the formation of polymer by combining all elements of the MIP and allowing the molecular interactions to form the cross-linked polymer with the template molecule bound. The second method of formation of MIPs involves covalently linking the imprint molecule to the monomer. After polymerization, the monomer is cleaved from the template molecule. The selectivity is greatly influenced by the kind and amount of cross-linking agent used in the synthesis of the imprinted polymer. The selectivity is also determined by the covalent and non-covalent interactions between the target molecule and monomer functional groups. The careful choice of functional monomer is another important choice to provide complementary interactions with the template and substrates. In an imprinted polymer, the cross-linker fulfills three major functions: First of all, the cross-linker is important in controlling the morphology of the polymer matrix, whether it is gel-type, macroporous or a microgel powder. Secondly, it serves to stabilize the imprinted binding site. Finally, it imparts mechanical stability to the polymer matrix. From a polymerization point of view, high cross-link ratios are generally preferred in order to access permanently porous materials and in order to be able to generate materials with adequate mechanical stability. 

The self-assembly method has advantages in the fact that it forms a more natural binding site, and also offers additional flexibility in the types of monomers that can be polymerized. The covalent method has its advantages in generally offering a high yield of homogeneous binding sites, but first requires the synthesis of a derivatized imprint molecule and may not imitate the "natural" conditions that could be present elsewhere. Over the recent years, interest in the technique of molecular imprinting has increased rapidly, both in the academic community and in the industry. Consequently, significant progress has been made in developing polymerization methods that produce adequate MIP formats with rather good binding properties expecting an enhancement in the performance or in order to suit the desirable final application, such as beads, films or nanoparticles. One of the key issues that have limited the performance of MIPs in practical applications so far is the lack of simple and robust methods to synthesize MIPs in the optimum formats required by the application. Chronologically, the first polymerization method encountered for MIP was based on "bulk" or solution polymerization. This method is the most common technique used by groups working on imprinting especially due to its simplicity and versatility. It is used exclusively with organic solvents mainly with low dielectric constant and consists basically of mixing all the components (template, monomer, solvent and initiator) and subsequently polymerizing them. The resultant polymeric block is then pulverized, freed from the template, crushed and sieved to obtain particles of irregular shape and size between 20 and 50 µm. Depending on the target (template) type and the final application of the MIP, MIPs are appeared in different formats such as nano/micro spherical particles, nanowires and thin film or membranes. They are produced with different polymerization techniques like bulk, precipitation, emulsion, suspension, dispersion, gelation, and multi-step swelling polymerization. Most of investigators in the field of MIP are making MIP with heuristic techniques such as hierarchical imprinting method. The technique for the first time was used for making MIP by Sellergren et al. for imprinting small target molecules. With the same concept, Nematollahzadeh et al. developed a general technique, so-called polymerization packed bed, to obtain hierarchically-structured, high capacity protein imprinted porous polymer beads by using silica porous particles for protein recognition and capture.

Solid-phase synthesis

Solid-phase molecular imprinting has been recently developed as an alternative to traditional bulk imprinting, generating water-soluble nanoparticles. As the name implies, this technique requires the immobilisation of the target molecule on a solid support prior to performing polymerisation. This is analogous to solid-phase synthesis of peptides. The solid phase doubles as an affinity separation matrix, allowing the removal of low-affinity MIPs and overcoming many of the previously described limitations of MIPs:
  • Separation of MIPs from the immobilised template molecule is greatly simplified.
  • Binding sites are more uniform, and template molecules cannot become trapped within the polymer matrix.
  • MIPs can be functionalised post-synthesis (whilst attached to the solid phase) without significantly influencing binding sites.
  • The immobilised template can be reused, reducing the cost of MIP synthesis.
MIP nanoparticles synthesised via this approach have found applications in various diagnostic assay and sensors.

Molecular modelling

Molecular modelling has become a convenient choice in MIP design and analysis, allowing rapid selection of monomers and optimisation of polymer composition, with a range of different techniques being applied. The application of molecular modelling in this capacity is commonly attributed to Sergey A. Piletsky, who developed a method of automated screening of a large database of monomers against a given target or template with a molecular mechanics approach. In recent years technological advances have permitted more efficient analysis of monomer-template interactions by quantum mechanical molecular modelling, providing more precise calculations of binding energies. Molecular dynamics has also been applied for more detailed analysis of systems before polymerisation, and of the resulting polymer, which by including more system components (cross-linkers, solvents) provide greater accuracy in predicting successful MIP synthesis than monomer-template interactions alone. Molecular modelling, particular molecular dynamics and the less common coarse-grained techniques, can often also be integrated into greater theoretical models permitting thermodynamic analysis and kinetic data for mesoscopic analysis of imprinted polymer bulk monoliths and MIP nanoparticles.

Applications

Niche areas for application of MIPs are in sensors and separation. Despite the current good health of molecular imprinting in general, one difficulty which appears to remain to this day is the commercialization of molecularly imprinted polymers. Despite this, many patents (1035 patents, up to October 2018, according to the Scifinder data base) on molecular imprinting were held by different groups. Commercial interest is also confirmed by the fact that MIP Technologies, offers a range of commercially available MIP products and Sigma-Aldrich produces SupelMIP for beta-agonists, beta-blockers, pesticides and some drugs of abuse such as amphetamine. Additionally, POLYINTELL designs, manufactures and markets AFFINIMIPSPE products for instance for mycotoxins such as patulin, zearalenone, fumonisins, ochratoxin A, for endocrine disruptors (bisphenol A, estrogen derivatives etc...) or for the purification of radiotracers before their use in positron emission tomography (PET).

Fast and cost-effective molecularly imprinted polymer technique has applications in many fields of chemistry, biology and engineering, particularly as an affinity material for sensors, detection of chemical, antimicrobial, and dye, residues in food, adsorbents for solid phase extraction, binding assays, artificial antibodies, chromatographic stationary phase, catalysis, drug development and screening, and byproduct removal in chemical reaction. Molecular imprinted polymers pose this wide range of capabilities in extraction through highly specific micro-cavity binding sites. Due to the specific binding site created in a MIP this technique is showing promise in analytical chemistry as a useful method for solid phase extraction. The capability for MIPs to be a cheaper easier production of antibody/enzyme like binding sites doubles the use of this technique as a valuable breakthrough in medical research and application. Such possible medical applications include "controlled release drugs, drug monitoring devices, and biological receptor mimetics". Beyond this MIPs show a promising future in the developing knowledge and application in food sciences.

"Plastic antibodies" The binding activity of MIPs can be two magnitudes of activity lower than that of specific antibodies. These binding sites, though not as strong as antibodies, are still highly specific that can be made easily and relatively cheaply. This yields a wide variety of applications for MIPs from efficient extraction to pharmaceutical/medical uses. MIPs offer many advantages over protein binding sites. Proteins are difficult and expensive to purify, denature (pH, heat, proteolysis), and are difficult to immobilize for reuse. Synthetic polymers are cheap, easy to synthesize, and allow for elaborate, synthetic side chains to be incorporated. Unique side chains allow for higher affinity, selectivity, and specificity. 

Molecularly imprinted assays Molecularly imprinted polymers arguably demonstrate their greatest potential as alternative affinity reagents for use in diagnostic applications, due to their comparable (and in some regards superior) performance to antibodies. Many studies have therefore focused on the development of molecularly imprinted assays (MIAs) since the seminal work by Vlatakis et al. in 1993, where the term “molecularly imprinted [sorbet] assay” was first introduced. Initial work on ligand binding assays utilising MIPs in place of antibodies consisted of radio-labelled MIAs, however the field has now evolved to include numerous assay formats such as fluorescence MIAs, enzyme-linked MIAs, and molecularly imprinted nanoparticle assay (MINA).

Molecularly imprinted polymers have also been used to enrich low abundant phosphopeptides from a cell lysate, outperforming titanium dioxide (TiO2) enrichment- a gold standard to enrich phosphopeptides.

History

In a paper published in 1931, Polyakov reported the effects of presence of different solvents (benzene, toluene and xylene) on the silica pore structure during drying a newly prepared silica. When H2SO4 was used as the polymerization initiator (acidifying agent), a positive correlation was found between surface areas, e.g. load capacities, and the molecular weights of the respective solvents. Later on, in 1949 Dickey reported the polymerization of sodium silicate in the presence of four different dyes (namely methyl, ethyl, n-propyl and n-butyl orange). The dyes were subsequently removed, and in rebinding experiments it was found that silica prepared in the presence of any of these "pattern molecules" would bind the pattern molecule in preference to the other three dyes. Shortly after this work had appeared, several research groups pursued the preparation of specific adsorbents using Dickey's method. Some commercial interest was also shown by the fact that Merck patented a nicotine filter, consisting of nicotine imprinted silica, able to adsorb 10.7% more nicotine than non-imprinted silica. The material was intended for use in cigarettes, cigars and pipes filters. Shortly after this work had appeared, molecular imprinting attracted wide interest from the scientific community as reflected in the 4000 original papers published in the field during for the period 1931–2009 (from Scifinder). However, although interest in the technique is new, commonly the molecularly imprinted technique has been shown to be effective when targeting small molecules of molecular weight less than 1000. Therefore, in following subsection molecularly imprinted polymers are reviewed into two categories, for small and big templates.

Production limitations

Production of novel MIPs has implicit challenges unique to this field. These challenges arise chiefly from the fact that all substrates are different and thus require different monomer and cross-linker combinations to adequately form imprinted polymers for that substrate. The first, and lesser, challenge arises from choosing those monomers which will yield adequate binding sites complementary to the functional groups of the substrate molecule. For example, it would be unwise to choose completely hydrophobic monomers to be imprinted with a highly hydrophilic substrate. These considerations need to be taken into account before any new MIP is created. Molecular modelling can be used to predict favourable interactions between templates and monomers, allowing intelligent monomer selection.

Secondly, and more troublesome, the yield of properly created MIPs is limited by the capacity to effectively wash the substrate from the MIP once the polymer has been formed around it. In creating new MIPs, a compromise must be created between full removal of the original template and damaging of the substrate binding cavity. Such damage is generally caused by strong removal methods and includes collapsing of the cavity, distorting the binding points, incomplete removal of the template and rupture of the cavity. 

Challenges of Template Removal for Molecular Imprinted Polymers

Template removal

Most of the developments in MIP production during the last decade have come in the form of new polymerization techniques in an attempt to control the arrangement of monomers and therefore the polymers structure. However, there have been very few advances in the efficient removal of the template from the MIP once it has been polymerized. Due to this neglect, the process of template removal is now the least cost efficient and most time consuming process in MIP production. Furthermore, in order of MIPs to reach their full potential in analytical and biotechnological applications, an efficient removal process must be demonstrated. 

There are several different methods of extraction which are currently being used for template removal. These have been grouped into 3 main categories: Solvent extraction, physically assisted extraction, and subcritical or supercritical solvent extraction.

Solvent extraction

  • Soxhlet extraction This has been a standard extraction method with organic solvents since its creation over a century ago. This technique consists of placing the MIP particles into a cartridge inside the extraction chamber, and the extraction solvent in poured into a flask connected to the extractor chamber. The solvent is then heated and condenses inside the cartridge thereby contacting the MIP particles and extracting the template. The main advantages to this technique are the repeated washing of MIP particles with fresh extracting solvent, favors solubilization because it uses hot solvent, no filtration is required upon completion to collect the MIP particles, the equipment is affordable, and it is very versatile and can be applied to nearly any polymer matrix. The main disadvantages are the long extraction time, the large amount of organic solvent used, the possibility or degradation for temperature sensitive polymers, the static nature of the technique does not facilitate solvent flow through MIP, and the automation is difficult.
  • Incubation This involves the immersion of the MIPs into solvents that can induce swelling of the polymer network and simultaneously favor the dissociation of the template from the polymer. Generally this method is carried out under mild conditions and the stability of the polymer is not affected. However, much like the Soxhlet extraction technique, this method also is very time consuming.
  • Solid-phase template As described above, one benefit of immobilising the template molecule on a solid support such as glass beads is the easy removal of the MIPs from the template. Following a cold wash to remove unreacted monomers and low-affinity polymers, hot solvent can be added to disrupt binding and allow the collection of high affinity MIPs.

Physically-assisted extraction

  • Ultrasound-assisted extraction (UAE) This method uses Ultrasound which is a cyclic sound pressure with a frequency greater than 20 kHz. This method works through the process known as cavitation which forms small bubbles in liquids and the mechanical erosion of solid particles. This causes a local increase in temperature and pressure which favor solubility, diffusivity, penetration and transport of solvent and template molecules.
  • Microwave-assisted extraction (MAE) This method uses microwaves which directly interact with the molecules causing Ionic conduction and dipole rotation. The use of microwaves for extraction make the extraction of the template occur rapidly, however, one must be careful to avoid excessively high temperatures if the polymers are heat sensitive. This has the best results when the technique is used in concert with strong organic acids, however, this poses another problem because it may cause partial MIP degradation as well. This method does have some benefits in that it significantly reduces the time required to extract the template, decreases the solvent costs, and is considered to be a clean technique.
  • Mechanical method A study has shown that the microcontact molecular imprinting method allows mechanical removal of the target (large biomolecules, proteins etc.) from the template. This technology combined with biosensor applications is promising for biotechnological, environmental and medical applications.

Subcritical or supercritical solvent extraction

  • Subcritical water (PHWE) This method employs the use of water, which is the cheapest and greenest solvent, under high temperatures (100–374 C) and pressures ( 10–60 bar). This method is based upon the high reduction in polarity that liquid water undergoes when heated to high temperatures. This allows water to solubilize a wide variety of polar, ionic and non-polar compounds. The decreased surface tension and viscosity under these conditions also favor diffusivity. Furthermore, the high thermal energy helps break intermolecular forces such as dipole-dipole interactions, vander Waals forces, and hydrogen bonding between the template and the matrix.
  • Supercritical CO2 (SFE)

Artificial enzyme

From Wikipedia, the free encyclopedia

Schematic drawing of artificial phosphorylase
 
An artificial enzyme is a synthetic, organic molecule or ion that recreate some function of an enzyme. The area promises to deliver catalysis at rates and selectivity observed in many enzymes.

History

Enzyme catalysis of chemical reactions occur with high selectivity and rate. The substrate is activated in a small part of the enzyme's macromolecule called the active site. There, the binding of a substrate close to functional groups in the enzyme causes catalysis by so-called proximity effects. It is possible to create similar catalysts from small molecule by combining substrate-binding with catalytic functional groups. Classically artificial enzymes bind substrates using receptors such as cyclodextrin, crown ethers, and calixarene.

Artificial enzymes based on amino acids or peptides as characteristic molecular moieties have expanded the field of artificial enzymes or enzyme mimics. For instance, scaffolded histidine residues mimics certain metalloproteins and -enzymes such as hemocyanin, tyrosinase, and catechol oxidase).

Artificial enzymes have been designed from scratch via a computational strategy using Rosetta. In December 2014, it was announced that active enzymes had been produced that were made from artificial molecules which do not occur anywhere in nature. In 2017, a book chapter entitled "Artificial Enzymes: The Next Wave" was published.

Nanozymes

Nanozymes are nanomaterials with enzyme-like characteristics. They have been widely explored for various applications, such as biosensing, bioimaging, tumor diagnosis and therapy, antibiofouling.

1990s

In 1996 and 1997, Dugan et al. discovered the superoxide dismutase (SOD) mimicking activities of fullerene derivatives.

2000s

In 2004, the term "nanozymes" was coined by Flavio Manea, Florence Bodar Houillon, Lucia Pasquato, and Paolo Scrimin. In 2006, nanoceria (i.e., CeO2 nanoparticles) was used for preventing retinal degeneration induced by intracellular peroxides. In 2007, Xiyun Yan and coworkers reported that ferromagnetic nanoparticles possessed intrinsic peroxidase-like activity. In 2008, Hui Wei and Erkang Wang developed an iron oxide nanozyme based sensing platform for bioactive molecules (such as hydrogen peroxide and glucose).

2010s

In 2010 and 2011, graphene oxide with peroxidase-like activity was reported. In 2012, recombinant human heavy-chain ferritin coated iron oxide nanoparticle with peroxidase-like activity was prepared and used for targeting and visualizing tumour tissues. In 2012, vanadium pentoxide nanoparticles with vanadium haloperoxidase mimicking activities were used for preventing marine biofouling. In 2014, it was demonstrated that carboxyfullerene could be used to treat neuroprotection postinjury in Parkinsonian nonhuman primates. Peroxidase-like polyoxometalate derivatives were developed as functional anti-amyloid agents for Alzheimer’s disease. V2O5 nanozymes with cytoprotective function was reported. In 2015, a supramolecular regulation strategy was proposed to modulate the activity of gold-based nanozymes for imaging and therapeutic applications. A nanozyme-strip for rapid local diagnosis of Ebola was developed. Nanoceria nanozymes were used for DNA sensing. An integrated nanozyme has been developed for real time monitoring the dynamic changes of cerebral glucose in living brains. Cu(OH)2 nanozymes with peroxidase-like activities were reported. Ionic FePt, Fe3O4, Pd, and CdSe NPs with peroxidase-like activities were reported. A book entitled "Nanozymes: Next Wave of Artificial Enzymes" was published. A book chapter entitled "Nanozymes" in the book of "Enzyme Engineering" was published (in Chinese). Oxidase-like nanoceria has been used for developing self-regulated bioassays. Multi-enzyme mimicking Prussian blue was developed for therapeutics. Histidine was used to modulate iron oxide nanoparticles' peroxidase mimicking activities. Gold nanoparticles' peroxidase mimicking activities were modulated via a supramolecular strategy for cascade reactions. A molecular imprinting strategy was developed to improve the selectivity of Fe3O4 nanozymes with peroxidase-like activity. A new strategy was developed to enhance the peroxidase mimicking activity of gold nanoparticles by using hot electrons. Researchers have designed gold nanoparticles (AuNPs) based integrative nanozymes with both SERS and peroxidase mimicking activities for measuring glucose and lactate in living tissues. Cytochrome c oxidase mimicking activity of Cu2O nanoparticles was modulated by receiving electrons from cytochrome c. Fe3O4 NPs were combined with glucose oxidase for tumor therapeutics. Manganese dioxide nanozymes have been used as cytoprotective shells. Mn3O4 Nanozyme for Parkinson's Disease (cellular model) was reported. Heparin elimination in live rats has been monitored with 2D MOF based peroxidase mimics and AG73 peptide. Glucose oxidase and iron oxide nanozymes were encapsulated within multi-compartmental hydrogels for incompatible tandem reactions. A cascade nanozyme biosensor was developed for detection of viable Enterobacter sakazakii. An integrated nanozyme of GOx@ZIF-8(NiPd) was developed for tandem catalysis. Charge-switchable nanozymes were developed. Site-selective RNA splicing nanozyme was developed. A nanozymes special issue in Progress in Biochemistry and Biophysics was published. Mn3O4 nanozymes with ROS scavenging activities have been developed for in vivo anti-inflammation. A concept entitled "A Step into the Future – Applications of Nanoparticle Enzyme Mimics" was proposed. Facet-dependent oxidase and peroxidase-like activities of Pd nanoparticles were reported. Au@Pt multibranched nanostructures as bifunctional nanozymes were developed. Ferritin coated carbon nanozymes were developed for tumor catalytic therapy. CuO nanozymes were developed to kill bacteria via a light-controlled manner. Enzymatic activity of oxygenated CNT was studied. Nanozymes were used to catalyze the oxidation of l-Tyrosine and l-Phenylalanine to dopachrome. Nanozyme as an emerging alternative to natural enzyme for biosensing and immunoassay was summarized. Standardized assay was proposed for peroxidase-like nanozymes. Semiconductor QDs as nucleases for site-selective photoinduced cleavage of DNA. 2D-MOF nanozyme-based sensor arrays was constructed for detecting phosphates and probing their enzymatic hydrolysis. N-doped carbon nanomaterials as specific peroxidase mimics were reported. Nanozyme sensor arrays were developed to detect analytes from small Molecules to proteins and cells. Copper oxide nanozyme for Parkinson’s Disease was reported. Exosome-like nanozyme vesicles for tumor Imaging was developed. A comprehensive review on nanozymes was published by Chemical Society Reviews. A progress report on nanozymes was published. eg occupancy as an effective descriptor was developed for the catalytic activity of perovskite oxide-based peroxidase mimics. A Chemical Reviews on nanozymes was published. A single-atom strategy was used for developing nanozymes. Nanozyme for metal-free bioinspired cascade photocatalysis was reported.

Conferences

Several conferences have focused on nanozymes. In 2015, a nanozyme workshop for was held at the 9th Asian Biophysics Associatation (ABA) Symposium. In Pittcon 2016, a Networking entitled "Nanozymes in Analytical Chemistry and Beyond" was devoted to nanozymes. An Xiangshan Science Conference was devoted to nanozyme research. A scientific session was devoted to "Biomimetic Nanocatalysis" in 15th Chinese Biophysics Congress. The "Nanozymes for Bioanalysis (Oral)" section was included in the 256th ACS National Meeting (2018 Fall, Boston).

Nanoparticle–biomolecule conjugate

From Wikipedia, the free encyclopedia

Attachments on nanoparticles make them more biocompatible.
 
A nanoparticle–biomolecule conjugate is a nanoparticle with biomolecules attached to its surface. Nanoparticles are minuscule particles, typically measured in nanometers (nm), that are used in nanobiotechnology to explore the functions of biomolecules. Properties of the ultrafine particles are characterized by the components on their surfaces more so than larger structures, such as cells, due to large surface area-to-volume ratios. Large surface area-to-volume-ratios of nanoparticles optimize the potential for interactions with biomolecules.

Characterization

Major characteristics of nanoparticles include volume, structure, and visual properties that make them valuable in nanobiotechnology. Depending on specific properties of size, structure, and luminescence, nanoparticles can be used for different applications. Imaging techniques are used to identify such properties and give more information about the tested sample. Techniques used to characterize nanoparticles are also useful in studying how nanoparticles interact with biomolecules, such as amino acids or DNA, and include magnetic resonance imaging (MRI), denoted by the solubility of the nanoparticles in water and fluorescent. MRI can be applied in the medical field to visualize structures; atomic force microscopy (AFM) that gives a topographic view of the sample on a substrate; transmission electron microscopy (TEM) that gives a top view, but with a different technique then that of atomic force microscopy; Raman spectroscopy or surface enhanced Raman spectroscopy (SERS) gives information about wavelengths and energy in the sample. ultraviolet-visible spectroscopy (UV-Vis) measures the wavelengths where light is absorbed; X-ray diffraction (XRD) generally gives an idea of the chemical composition of the sample.

Chemistry

Physical

Nanomolecules can be created from virtually any element, but the majority produced in today's industry use carbon as the basis upon which the molecules are built around. Carbon can bond with nearly any element, allowing many possibilities when it comes to creating a specific molecule. Scientists can create thousands upon thousands of individual nanomolecules from a simple carbon basis. Some of the most famous nanomolecules currently in existence are solely carbon; these include carbon nanotubes and buckminsterfullerenes. In contrast with nanomolecules, the chemical components of nanoparticles usually consist of metals, such as iron, gold, silver, and platinum.

Interactions between nanoparticles and molecules change depending on the nanoparticle's core. Nanoparticle properties depend not only on the composition of the core material, but also on varying thicknesses of material used. Magnetic properties are particularly useful in molecule manipulation, and thus metals are often used as core material. Metals contain inherent magnetic properties that allow for manipulation of molecular assembly. As nanoparticles interact with molecules via ligand properties, molecular assembly can be controlled by external magnetic fields interacting with magnetic properties in the nanoparticles. Significant problems with producing nanoparticles initially arise once these nanoparticles are generated in solution. Without the use of a stabilizing agent, nanoparticles tend to stick together once the stirring is stopped. In order to counteract this, a certain collidial stabilizer is generally added. These stabilizers bind to the nanoparticles in a way that prevents other particles from bonding with them. Some effective stabilizers found so far include citrate, cellulose, and sodium borohydride.

Application chemistry

Nanoparticles are desirable in today's industry for their high surface area-to-volume ratio in comparison with larger particles of the same elements. Because chemical reactions occur at a rate directly proportional to the available surface area of reactant compounds, nanoparticles can generate reactions at a much faster rate than larger particles of equal mass. Nanoparticles therefore are among the most efficient means of producing reactions and are inherently valuable in the chemical industry. The same property makes them valuable in interactions with molecules.

Applications with biomolecules and biological processes

Nanoparticles have the potential to greatly influence biological processes. The potency of a nanoparticle increases as its surface area-to-volume-ratio does. Attachments of ligands to the surface of nanoparticles allow them to interact with biomolecules.

Identification of biomolecules

Nanoparticles are valuable tools in identification of biomolecules, through the use of bio-tagging or labeling. Attachments of ligands or molecular coatings to the surface of a nanoparticle facilitate nanoparticle-molecule interaction, and make them biocompatible. Conjugation can be achieved through intermolecular attractions between the nanoparticle and biomolecule such as covalent bonding, chemisorption, and noncovalent interactions.

To enhance visualization, nanoparticles can also be made to fluoresce by controlling the size and shape of a nanoparticle probe. Fluorescence increases luminescence by increasing the range of wavelengths the emitted light can reach, allowing for biomarkers with a variety of colors. This technique is used to track the efficacy of protein transfer both in vivo and in vitro in terms of genetic alternations.

Biological process control

Biological processes can be controlled through transcription regulation, gene regulation, and enzyme inhibition processes that can be regulated using nanoparticles. Nanoparticles can play a part in gene regulation through ionic bonding between positively charged cationic ligands on the surfaces of nanoparticles and negatively charged anionic nucleic acids present in DNA. In an experiment, a nanoparticle-DNA complex inhibited transcription by T7 RNA polymerase, signifying strong bonding in the complex. A high affinity of the nanoparticle-DNA complex indicates strong bonding and a favorable use of nanoparticles. Attaching ionic ligands to nanoparticles allows control over enzyme activity. An example of enzyme inhibition is given by binding of a-chymotrypsin (ChT), an enzyme with a largely cationic active site. When a-chymotrypsin is incubated with anionic (negatively charged) nanoparticles, ChT activity is inhibited as anionic nanoparticles bind to the active site. Enzyme activity can be restored by the addition of cationic surfactants. Alkyl surfactants form a bilayer around ChT, whereas thiol and alcohol surfactants alter the surface of ChT such that interactions with nanoparticles are interrupted. Though formation of a protein-nanoparticle complex can inhibit enzyme activity, studies show that it can also stabilize protein structure, and significantly protect the protein from denaturization. Attachments of ligands to segments of nanoparticles selected for functionalization of metallic properties can be used to generate a magnetic nanowire, which generates a magnetic field that allows for the manipulation of cellular assemblies.

Genetic alteration

Nanoparticles can also be used in conjunction with DNA to perform genetic alterations. These are frequently monitored through the use of fluorescent materials, allowing scientists to judge if these tagged proteins have successfully been transmitted—for example green fluorescent protein, or GFP. Nanoparticles are significantly less cytotoxic than currently used organic methods, providing a more efficient method of monitoring genetic alternations. They also do not degrade or bleach with time, as organic dyes do. Suspensions of nanoparticles with the same size and shapes (mono-dispersed) with functional groups attached to their surfaces can also electrostatically bind to DNA, protecting them from several types of degradation. Because the fluorescence of these nanoparticles does not degrade, cellular localization can be tracked without the use of additional tagging, with GFPs or other methods. The 'unpacking' of the DNA can be detected in live cells using luminescence resonance energy transfer (LRET) technology.

Medical implications

Small molecules in vivo have a short retention time, but the use of larger nanoparticles does not. These nanoparticles can be used to avoid immune response, which aids in the treatment of chronic diseases. It has been investigated as a potential cancer therapy and also has the potential to affect the understanding of genetic disorders. Nanoparticles also have the potential to aid in site-specific drug delivery by improving the amount of unmodified drug that is circulated within the system, which also decreases the necessary dosage frequency. The targeted nature of nanoparticles also means that non-targeted organs are much less likely to experience side effects of drugs intended for other areas.

Studying cell interactions

Cellular interactions occur at a microscopic level and cannot be easily observed even with the advanced microscopes available today. Due to difficulties observing reactions at the molecular level, indirect methods are used which greatly limits the scope of the understanding that can be gained by studying these processes essential to life. Advances in the material industry has evolved a new field known as nanobiotechnology, that uses nanoparticles to study interactions at the biomolecular level.

One area of research featuring nanobiotechnology is the extracellular matrices of cells (ECM). The ECM is primarily composed of interwoven fibers of collagen and elastin that have diameters ranging from 10–300 nm. In addition to holding the cell in place, the ECM has a variety of other functions including providing a point of attachment for the ECM of other cells and transmembrane receptors that are essential for life. Until recently it has been nearly impossible to study the physical forces that help cells maintain their functionality, but nanobiotechnology has given us the ability to learn more about these interactions. Using the unique properties of nanoparticles, it is possible to control how the nanoparticles adhere to certain patterns present in the ECM, and as a result can understand how changes in the ECM's shape can affect cell functionality.

Using nanobiotechnology to study the ECM allows scientists to investigate the binding interactions that occur between the ECM and its supporting environment. Investigators were able to study these interactions by utilizing tools such as optical tweezers, which have the ability to trap nano-scale objects with focused light. The tweezers can affect the binding of a substrate to the ECM by attempting to draw the substrate away from it. The light emitted from the tweezers was used to restrain ECM-coated microbeads, and the changes in the force exerted by the ECM onto the substrate were studied by modulating the effect of the optical tweezers. Experiments showed that the force exerted by the ECM on the substrate positively correlated with the force of the tweezers, which led to the subsequent discovery that the ECM and the transmembrane proteins are able to sense external forces, and can adapt to overcome these forces.

Nanotechnology crossing the blood-brain barrier

The blood-brain barrier (BBB) is composed of a system of capillaries that has an especially dense lining of endothelial cells which protects the central nervous system (CNS) against the diffusion of foreign substances into the cerebrospinal fluid. These harmful objects include microscopic bacteria, large hydrophobic molecules, certain hormones and neurotransmitters, and low-lipid-soluble molecules. The BBB prevents these harmful particles from entering the brain via tight junctions between endothelial cells and metabolic barriers. The thoroughness with which the BBB does its job makes it difficult to treat diseases of the brain such as cancer, Alzheimer's, and autism, because it is very difficult to transport drugs across the BBB. Currently, in order to deliver therapeutic molecules into the brain, doctors must use highly invasive techniques such as drilling directly into the brain, or sabotaging the integrity of the BBB through biochemical means. Due to their small size and large surface area, nanoparticles offer a promising solution for neurotherapeutics. 

Nanotechnology is helpful in delivering drugs and other molecules across the blood-brain barrier (BBB). Nanoparticles allow drugs, or other foreign molecules, to efficiently cross the BBB by camouflaging themselves and tricking the brain into providing them with the ability to cross the BBB in a process called the Trojan Horse Method. Using nanotechnology is advantageous because only the engineered complex is necessary whereas in ordinary applications the active compound must carry out the reaction. This allows for maximum efficacy of the active drug. Also, the use of nanoparticles results in the attraction of proteins to the surfaces of cells, giving cell membranes a biological identity. They also use endogenous active transport where transferrin, an iron binding protein, is linked to rod-shaped semiconductor nanocrystals, in order to move across the BBB into the brain. This discovery is a promising development towards designing an efficient nanoparticle-based drug delivery system.

Introduction to entropy

From Wikipedia, the free encyclopedia https://en.wikipedia.org/wiki/Introduct...