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When using the term 'the speed of light' it is sometimes necessary to make the distinction between its one-way speed and its two-way speed. The "one-way" speed of light, from a source to a detector, cannot be measured independently of a convention as to how to synchronize the clocks at the source and the detector. What can however be experimentally measured is the round-trip speed (or "two-way" speed of light) from the source to the detector and back again. Albert Einstein chose a synchronization convention (see Einstein synchronization) that made the one-way speed equal to the two-way speed. The constancy of the one-way speed in any given inertial frame is the basis of his special theory of relativity, although all experimentally verifiable predictions of this theory do not depend on that convention.

Experiments that attempted to directly probe the one-way speed of light independent of synchronization have been proposed, but none has succeeded in doing so. Those experiments directly establish that synchronization with slow clock-transport is equivalent to Einstein synchronization, which is an important feature of special relativity. Though those experiments don't directly establish the isotropy of the one-way speed of light, because it was shown that slow clock-transport, the laws of motion, and the way inertial reference frames are defined, already involve the assumption of isotropic one-way speeds and thus are conventional as well. In general, it was shown that these experiments are consistent with anisotropic one-way light speed as long as the two-way light speed is isotropic.

The 'speed of light' in this article refers to the speed of all electromagnetic radiation in vacuum.

The two-way speed